Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide
MAP kinases have been linked to guard cell signalling. Arabidopsis thaliana MAP Kinase 3 (MPK3) is known to be activated by abscisic acid (ABA) and hydrogen peroxide (H2O2), which also control stomatal movements. We therefore studied the possible role of MPK3 in guard cell signalling through guard c...
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todo:paper_0028646X_v173_n4_p713_Gudesblat2023-10-03T14:39:08Z Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide Gudesblat, G.E. Iusem, N.D. Morris, P.C. Abscisic acid (ABA) Arabidopsis thaliana Hydrogen peroxide (H 2O2) MAP kinase MPK3 Stomata abscisic acid Arabidopsis protein complementary DNA hydrogen peroxide mitogen activated protein kinase 3 abnormality abscisic acid alkalinization enzyme activity gene expression hydrogen peroxide inhibition RNA signal stomatal conductance transgenic plant Arabidopsis article cytology genetic transformation genetics metabolism physiology plant epidermis plant leaf signal transduction transgenic plant Abscisic Acid Arabidopsis Arabidopsis Proteins DNA, Antisense Hydrogen Peroxide Mitogen-Activated Protein Kinase 3 Plant Epidermis Plant Leaves Plants, Genetically Modified Signal Transduction Transformation, Genetic Arabidopsis Arabidopsis thaliana MAP kinases have been linked to guard cell signalling. Arabidopsis thaliana MAP Kinase 3 (MPK3) is known to be activated by abscisic acid (ABA) and hydrogen peroxide (H2O2), which also control stomatal movements. We therefore studied the possible role of MPK3 in guard cell signalling through guard cell-specific antisense inhibition of MPK3 expression. Such transgenic plants contained reduced levels of MPK3 mRNA in the guard cells and displayed partial insensitivity to ABA in inhibition of stomatal opening, but responded normally to this hormone in stomatal closure. However, ABA-induced stomatal closure was reduced compared with controls when cytoplasmic alkalinization was prevented with sodium butyrate. MPK3 antisense plants were less sensitive to exogenous H2O2, both in inhibition of stomatal opening and in promotion of stomatal closure, thus MPK3 is required for the signalling of this compound. ABA-induced H2O2 synthesis was normal in these plants, indicating that MPK3 probably acts in signalling downstream of H2O2. These results provide clear evidence for the important role of MPK3 in the perception of ABA and H 2O2 in guard cells. © The Authors (2007). Fil:Gudesblat, G.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Iusem, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0028646X_v173_n4_p713_Gudesblat |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Abscisic acid (ABA) Arabidopsis thaliana Hydrogen peroxide (H 2O2) MAP kinase MPK3 Stomata abscisic acid Arabidopsis protein complementary DNA hydrogen peroxide mitogen activated protein kinase 3 abnormality abscisic acid alkalinization enzyme activity gene expression hydrogen peroxide inhibition RNA signal stomatal conductance transgenic plant Arabidopsis article cytology genetic transformation genetics metabolism physiology plant epidermis plant leaf signal transduction transgenic plant Abscisic Acid Arabidopsis Arabidopsis Proteins DNA, Antisense Hydrogen Peroxide Mitogen-Activated Protein Kinase 3 Plant Epidermis Plant Leaves Plants, Genetically Modified Signal Transduction Transformation, Genetic Arabidopsis Arabidopsis thaliana |
| spellingShingle |
Abscisic acid (ABA) Arabidopsis thaliana Hydrogen peroxide (H 2O2) MAP kinase MPK3 Stomata abscisic acid Arabidopsis protein complementary DNA hydrogen peroxide mitogen activated protein kinase 3 abnormality abscisic acid alkalinization enzyme activity gene expression hydrogen peroxide inhibition RNA signal stomatal conductance transgenic plant Arabidopsis article cytology genetic transformation genetics metabolism physiology plant epidermis plant leaf signal transduction transgenic plant Abscisic Acid Arabidopsis Arabidopsis Proteins DNA, Antisense Hydrogen Peroxide Mitogen-Activated Protein Kinase 3 Plant Epidermis Plant Leaves Plants, Genetically Modified Signal Transduction Transformation, Genetic Arabidopsis Arabidopsis thaliana Gudesblat, G.E. Iusem, N.D. Morris, P.C. Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide |
| topic_facet |
Abscisic acid (ABA) Arabidopsis thaliana Hydrogen peroxide (H 2O2) MAP kinase MPK3 Stomata abscisic acid Arabidopsis protein complementary DNA hydrogen peroxide mitogen activated protein kinase 3 abnormality abscisic acid alkalinization enzyme activity gene expression hydrogen peroxide inhibition RNA signal stomatal conductance transgenic plant Arabidopsis article cytology genetic transformation genetics metabolism physiology plant epidermis plant leaf signal transduction transgenic plant Abscisic Acid Arabidopsis Arabidopsis Proteins DNA, Antisense Hydrogen Peroxide Mitogen-Activated Protein Kinase 3 Plant Epidermis Plant Leaves Plants, Genetically Modified Signal Transduction Transformation, Genetic Arabidopsis Arabidopsis thaliana |
| description |
MAP kinases have been linked to guard cell signalling. Arabidopsis thaliana MAP Kinase 3 (MPK3) is known to be activated by abscisic acid (ABA) and hydrogen peroxide (H2O2), which also control stomatal movements. We therefore studied the possible role of MPK3 in guard cell signalling through guard cell-specific antisense inhibition of MPK3 expression. Such transgenic plants contained reduced levels of MPK3 mRNA in the guard cells and displayed partial insensitivity to ABA in inhibition of stomatal opening, but responded normally to this hormone in stomatal closure. However, ABA-induced stomatal closure was reduced compared with controls when cytoplasmic alkalinization was prevented with sodium butyrate. MPK3 antisense plants were less sensitive to exogenous H2O2, both in inhibition of stomatal opening and in promotion of stomatal closure, thus MPK3 is required for the signalling of this compound. ABA-induced H2O2 synthesis was normal in these plants, indicating that MPK3 probably acts in signalling downstream of H2O2. These results provide clear evidence for the important role of MPK3 in the perception of ABA and H 2O2 in guard cells. © The Authors (2007). |
| format |
JOUR |
| author |
Gudesblat, G.E. Iusem, N.D. Morris, P.C. |
| author_facet |
Gudesblat, G.E. Iusem, N.D. Morris, P.C. |
| author_sort |
Gudesblat, G.E. |
| title |
Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide |
| title_short |
Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide |
| title_full |
Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide |
| title_fullStr |
Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide |
| title_full_unstemmed |
Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide |
| title_sort |
guard cell-specific inhibition of arabidopsis mpk3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide |
| url |
http://hdl.handle.net/20.500.12110/paper_0028646X_v173_n4_p713_Gudesblat |
| work_keys_str_mv |
AT gudesblatge guardcellspecificinhibitionofarabidopsismpk3expressioncausesabnormalstomatalresponsestoabscisicacidandhydrogenperoxide AT iusemnd guardcellspecificinhibitionofarabidopsismpk3expressioncausesabnormalstomatalresponsestoabscisicacidandhydrogenperoxide AT morrispc guardcellspecificinhibitionofarabidopsismpk3expressioncausesabnormalstomatalresponsestoabscisicacidandhydrogenperoxide |
| _version_ |
1807315580426911744 |