Presence of β-follicle-stimulating hormone and β-luteinizing hormone transcripts in the brain of Cichlasoma dimerus (Perciformes: Cichlidae): Effect of brain-derived gonadotropins on pituitary hormone release
Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play key roles in vertebrate gametogenesis and steroidogenesis. They are mainly synthesized in the pituitary gland. While investigating the ontogeny of FSH and LH cells in the cichlid fish Cichlasoma dimerus by immunohistochemistry (IHC...
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todo:paper_00283835_v89_n1_p27_Pandolfi2023-10-03T14:38:58Z Presence of β-follicle-stimulating hormone and β-luteinizing hormone transcripts in the brain of Cichlasoma dimerus (Perciformes: Cichlidae): Effect of brain-derived gonadotropins on pituitary hormone release Pandolfi, M. Pozzi, A.G. Cánepa, M. Vissio, P.G. Shimizu, A. Maggese, M.C. Lobo, G. Cichlids Immunohistochemistry Pituitary culture Reproduction follitropin gonadotropin growth hormone hypophysis hormone luteinizing hormone animal experiment animal tissue article controlled study female hormone release hormone transport male nonhuman priority journal protein expression real time polymerase chain reaction sequence analysis Western blotting Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play key roles in vertebrate gametogenesis and steroidogenesis. They are mainly synthesized in the pituitary gland. While investigating the ontogeny of FSH and LH cells in the cichlid fish Cichlasoma dimerus by immunohistochemistry (IHC), we unexpectedly found immunoreactive neurons in the preoptic area, sending their projections through different brain areas and neurohypophysis. Our previous work using Western blot and IHC techniques applied to the adult brain confirmed these findings. To further demonstrate the extrapituitary expression of these hormones, we performed RT-PCR detecting sequences coding for β-FSH and β-LH subunits in the C. dimerus pituitary and brain (preoptic-hypothalamic area). The expression of these transcripts in both organs was consistent with their peptide expression showing a high sequence homology when compared with other phylogenetically related fish. An individual pituitary in vitro culture system was utilized to study the possible modulatory effect of brain-derived gonadotropins on pituitary hormone secretion. Pituitary explants were cultured with different concentrations of LH or FSH, and the culture media were analyzed by Western blot. Exogenous LH produced a dose-dependent increase in pituitary β-LH, β-FSH and somatolactin (SL) releases. No effect was observed on growth hormone (GH). The effect on prolactin (PRL) was not consistent among treatments. Exogenous FSH produced an inhibition in β-LH release, dose-dependent increases in β-FSH and SL releases, and no effect on PRL and GH releases. These findings support the concept of regulation of pituitary trophic hormones by brain-derived gonadotropins. Copyright © 2008 S. Karger AG. Fil:Pandolfi, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pozzi, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cánepa, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vissio, P.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maggese, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lobo, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00283835_v89_n1_p27_Pandolfi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cichlids Immunohistochemistry Pituitary culture Reproduction follitropin gonadotropin growth hormone hypophysis hormone luteinizing hormone animal experiment animal tissue article controlled study female hormone release hormone transport male nonhuman priority journal protein expression real time polymerase chain reaction sequence analysis Western blotting |
| spellingShingle |
Cichlids Immunohistochemistry Pituitary culture Reproduction follitropin gonadotropin growth hormone hypophysis hormone luteinizing hormone animal experiment animal tissue article controlled study female hormone release hormone transport male nonhuman priority journal protein expression real time polymerase chain reaction sequence analysis Western blotting Pandolfi, M. Pozzi, A.G. Cánepa, M. Vissio, P.G. Shimizu, A. Maggese, M.C. Lobo, G. Presence of β-follicle-stimulating hormone and β-luteinizing hormone transcripts in the brain of Cichlasoma dimerus (Perciformes: Cichlidae): Effect of brain-derived gonadotropins on pituitary hormone release |
| topic_facet |
Cichlids Immunohistochemistry Pituitary culture Reproduction follitropin gonadotropin growth hormone hypophysis hormone luteinizing hormone animal experiment animal tissue article controlled study female hormone release hormone transport male nonhuman priority journal protein expression real time polymerase chain reaction sequence analysis Western blotting |
| description |
Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play key roles in vertebrate gametogenesis and steroidogenesis. They are mainly synthesized in the pituitary gland. While investigating the ontogeny of FSH and LH cells in the cichlid fish Cichlasoma dimerus by immunohistochemistry (IHC), we unexpectedly found immunoreactive neurons in the preoptic area, sending their projections through different brain areas and neurohypophysis. Our previous work using Western blot and IHC techniques applied to the adult brain confirmed these findings. To further demonstrate the extrapituitary expression of these hormones, we performed RT-PCR detecting sequences coding for β-FSH and β-LH subunits in the C. dimerus pituitary and brain (preoptic-hypothalamic area). The expression of these transcripts in both organs was consistent with their peptide expression showing a high sequence homology when compared with other phylogenetically related fish. An individual pituitary in vitro culture system was utilized to study the possible modulatory effect of brain-derived gonadotropins on pituitary hormone secretion. Pituitary explants were cultured with different concentrations of LH or FSH, and the culture media were analyzed by Western blot. Exogenous LH produced a dose-dependent increase in pituitary β-LH, β-FSH and somatolactin (SL) releases. No effect was observed on growth hormone (GH). The effect on prolactin (PRL) was not consistent among treatments. Exogenous FSH produced an inhibition in β-LH release, dose-dependent increases in β-FSH and SL releases, and no effect on PRL and GH releases. These findings support the concept of regulation of pituitary trophic hormones by brain-derived gonadotropins. Copyright © 2008 S. Karger AG. |
| format |
JOUR |
| author |
Pandolfi, M. Pozzi, A.G. Cánepa, M. Vissio, P.G. Shimizu, A. Maggese, M.C. Lobo, G. |
| author_facet |
Pandolfi, M. Pozzi, A.G. Cánepa, M. Vissio, P.G. Shimizu, A. Maggese, M.C. Lobo, G. |
| author_sort |
Pandolfi, M. |
| title |
Presence of β-follicle-stimulating hormone and β-luteinizing hormone transcripts in the brain of Cichlasoma dimerus (Perciformes: Cichlidae): Effect of brain-derived gonadotropins on pituitary hormone release |
| title_short |
Presence of β-follicle-stimulating hormone and β-luteinizing hormone transcripts in the brain of Cichlasoma dimerus (Perciformes: Cichlidae): Effect of brain-derived gonadotropins on pituitary hormone release |
| title_full |
Presence of β-follicle-stimulating hormone and β-luteinizing hormone transcripts in the brain of Cichlasoma dimerus (Perciformes: Cichlidae): Effect of brain-derived gonadotropins on pituitary hormone release |
| title_fullStr |
Presence of β-follicle-stimulating hormone and β-luteinizing hormone transcripts in the brain of Cichlasoma dimerus (Perciformes: Cichlidae): Effect of brain-derived gonadotropins on pituitary hormone release |
| title_full_unstemmed |
Presence of β-follicle-stimulating hormone and β-luteinizing hormone transcripts in the brain of Cichlasoma dimerus (Perciformes: Cichlidae): Effect of brain-derived gonadotropins on pituitary hormone release |
| title_sort |
presence of β-follicle-stimulating hormone and β-luteinizing hormone transcripts in the brain of cichlasoma dimerus (perciformes: cichlidae): effect of brain-derived gonadotropins on pituitary hormone release |
| url |
http://hdl.handle.net/20.500.12110/paper_00283835_v89_n1_p27_Pandolfi |
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