Activation of GABA B receptors in the anterior pituitary inhibits prolactin and luteinizing hormone secretion

Previous work from our laboratory showed that baclofen could lower serum prolactin (PRL) levels acting at the central nervous system. The present experiments were designed to evaluate whether the γ-aminobutyric acid B agonist was also effective in inhibiting hormone release at the pituitary level. I...

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Autores principales:	Lux-Lantos, V., Rey, E., Libertum, C.
Formato:	JOUR
Materias:	Adenohypophyseal cell cultures Baclofen Luteinizing hormone Prolactin 3 aminopropanephosphonic acid 4 aminobutanephosphonic acid 4 aminobutyric acid B receptor baclofen luteinizing hormone phaclofen prolactin protirelin adenohypophysis animal cell animal experiment article controlled study female gonadotropin release hypophysis cell luteinizing hormone release monolayer culture nonhuman priority journal prolactin release rat
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00283835_v56_n5_p687_LuxLantos
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00283835_v56_n5_p687_LuxLantos
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spelling	todo:paper_00283835_v56_n5_p687_LuxLantos2023-10-03T14:38:53Z Activation of GABA B receptors in the anterior pituitary inhibits prolactin and luteinizing hormone secretion Lux-Lantos, V. Rey, E. Libertum, C. Adenohypophyseal cell cultures Baclofen Luteinizing hormone Prolactin 3 aminopropanephosphonic acid 4 aminobutanephosphonic acid 4 aminobutyric acid B receptor baclofen luteinizing hormone phaclofen prolactin protirelin adenohypophysis animal cell animal experiment article controlled study female gonadotropin release hypophysis cell luteinizing hormone release monolayer culture nonhuman priority journal prolactin release rat Previous work from our laboratory showed that baclofen could lower serum prolactin (PRL) levels acting at the central nervous system. The present experiments were designed to evaluate whether the γ-aminobutyric acid B agonist was also effective in inhibiting hormone release at the pituitary level. In monolayer cultures of adenohypophyseal dispersed cells, baclofen inhibited basal PRL secetion after 1 or 2 h of incubation. This inhibition was significantly abolished by three antagonists: phaclofen, 3-aminopropyl-phosphonic acid and 4-aminobutylphosphonic acid. Furthermore, baclofen inhibited the thyrotropin-releasing hormone-induced PRL release in a concentration-dependent manner. With regard to gonadotropin secretion, baclofen was unable to modify basal luteinizing hormone (LH) secretion, but significantly inhibited the LH-releasing hormone-induced LH release. These results show that baclofen, in addition to its central neuroendocrine effects, inhibits pituitary hormone secretion, under basal and/or stimulated conditions, by direct action at the pituitary level. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00283835_v56_n5_p687_LuxLantos
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Adenohypophyseal cell cultures Baclofen Luteinizing hormone Prolactin 3 aminopropanephosphonic acid 4 aminobutanephosphonic acid 4 aminobutyric acid B receptor baclofen luteinizing hormone phaclofen prolactin protirelin adenohypophysis animal cell animal experiment article controlled study female gonadotropin release hypophysis cell luteinizing hormone release monolayer culture nonhuman priority journal prolactin release rat
spellingShingle	Adenohypophyseal cell cultures Baclofen Luteinizing hormone Prolactin 3 aminopropanephosphonic acid 4 aminobutanephosphonic acid 4 aminobutyric acid B receptor baclofen luteinizing hormone phaclofen prolactin protirelin adenohypophysis animal cell animal experiment article controlled study female gonadotropin release hypophysis cell luteinizing hormone release monolayer culture nonhuman priority journal prolactin release rat Lux-Lantos, V. Rey, E. Libertum, C. Activation of GABA B receptors in the anterior pituitary inhibits prolactin and luteinizing hormone secretion
topic_facet	Adenohypophyseal cell cultures Baclofen Luteinizing hormone Prolactin 3 aminopropanephosphonic acid 4 aminobutanephosphonic acid 4 aminobutyric acid B receptor baclofen luteinizing hormone phaclofen prolactin protirelin adenohypophysis animal cell animal experiment article controlled study female gonadotropin release hypophysis cell luteinizing hormone release monolayer culture nonhuman priority journal prolactin release rat
description	Previous work from our laboratory showed that baclofen could lower serum prolactin (PRL) levels acting at the central nervous system. The present experiments were designed to evaluate whether the γ-aminobutyric acid B agonist was also effective in inhibiting hormone release at the pituitary level. In monolayer cultures of adenohypophyseal dispersed cells, baclofen inhibited basal PRL secetion after 1 or 2 h of incubation. This inhibition was significantly abolished by three antagonists: phaclofen, 3-aminopropyl-phosphonic acid and 4-aminobutylphosphonic acid. Furthermore, baclofen inhibited the thyrotropin-releasing hormone-induced PRL release in a concentration-dependent manner. With regard to gonadotropin secretion, baclofen was unable to modify basal luteinizing hormone (LH) secretion, but significantly inhibited the LH-releasing hormone-induced LH release. These results show that baclofen, in addition to its central neuroendocrine effects, inhibits pituitary hormone secretion, under basal and/or stimulated conditions, by direct action at the pituitary level.
format	JOUR
author	Lux-Lantos, V. Rey, E. Libertum, C.
author_facet	Lux-Lantos, V. Rey, E. Libertum, C.
author_sort	Lux-Lantos, V.
title	Activation of GABA B receptors in the anterior pituitary inhibits prolactin and luteinizing hormone secretion
title_short	Activation of GABA B receptors in the anterior pituitary inhibits prolactin and luteinizing hormone secretion
title_full	Activation of GABA B receptors in the anterior pituitary inhibits prolactin and luteinizing hormone secretion
title_fullStr	Activation of GABA B receptors in the anterior pituitary inhibits prolactin and luteinizing hormone secretion
title_full_unstemmed	Activation of GABA B receptors in the anterior pituitary inhibits prolactin and luteinizing hormone secretion
title_sort	activation of gaba b receptors in the anterior pituitary inhibits prolactin and luteinizing hormone secretion
url	http://hdl.handle.net/20.500.12110/paper_00283835_v56_n5_p687_LuxLantos
work_keys_str_mv	AT luxlantosv activationofgababreceptorsintheanteriorpituitaryinhibitsprolactinandluteinizinghormonesecretion AT reye activationofgababreceptorsintheanteriorpituitaryinhibitsprolactinandluteinizinghormonesecretion AT libertumc activationofgababreceptorsintheanteriorpituitaryinhibitsprolactinandluteinizinghormonesecretion
_version_	1807323879851425792

Activation of GABA B receptors in the anterior pituitary inhibits prolactin and luteinizing hormone secretion

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