Testicular interstitial cells as targets for peripheral benzodiazepines
We evaluated the 'in vitro' effect of a selective peripheral benodiazepine (BZD) receptor agonist, Ro 5-4864, on basal and hCG-stimulated androgen production by testicular interstitial cell suspensions. Ro 5-4864 (10-9-10-5 M) induced a significant increment of basal testosterone release i...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00283835_v49_n3_p262_Ritta |
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todo:paper_00283835_v49_n3_p262_Ritta2023-10-03T14:38:52Z Testicular interstitial cells as targets for peripheral benzodiazepines Ritta, M.N. Calandra, R.S. 4' chlorodiazepam androgen benzodiazepine chorionic gonadotropin clonazepam diazepam n sec butyl 1 (2 chlorophenyl) n methyl 3 isoquinolinecarboxamide testosterone animal cell Leydig cell male nonhuman priority journal rat steroidogenesis testis topical drug administration We evaluated the 'in vitro' effect of a selective peripheral benodiazepine (BZD) receptor agonist, Ro 5-4864, on basal and hCG-stimulated androgen production by testicular interstitial cell suspensions. Ro 5-4864 (10-9-10-5 M) induced a significant increment of basal testosterone release into the medium. In addition, under conditions of hCG stimulation, Ro 5-4864 (10-7 M) induced a potentiated response to the gonadotropin in a dose-dependent manner. The selective peripheral BZD antagonist PK 11195 fully prevented the stimulatory effect of Ro 5-4864. On the other hand, clonazepam, a central BZD agonist, failed to affect androgen production significantly, whereas diazepam (10-5-10-4 M), which binds to both central and peripheral BZD receptors, was able to induce a significant increment of basal and hCG-stimulated testosterone production. These results suggest that under our experimental conditions Ro 5-4864 exerts an effect on testicular steroidogenesis, presumably through binding to the previously described peripheral-type BZD receptor. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00283835_v49_n3_p262_Ritta |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
4' chlorodiazepam androgen benzodiazepine chorionic gonadotropin clonazepam diazepam n sec butyl 1 (2 chlorophenyl) n methyl 3 isoquinolinecarboxamide testosterone animal cell Leydig cell male nonhuman priority journal rat steroidogenesis testis topical drug administration |
| spellingShingle |
4' chlorodiazepam androgen benzodiazepine chorionic gonadotropin clonazepam diazepam n sec butyl 1 (2 chlorophenyl) n methyl 3 isoquinolinecarboxamide testosterone animal cell Leydig cell male nonhuman priority journal rat steroidogenesis testis topical drug administration Ritta, M.N. Calandra, R.S. Testicular interstitial cells as targets for peripheral benzodiazepines |
| topic_facet |
4' chlorodiazepam androgen benzodiazepine chorionic gonadotropin clonazepam diazepam n sec butyl 1 (2 chlorophenyl) n methyl 3 isoquinolinecarboxamide testosterone animal cell Leydig cell male nonhuman priority journal rat steroidogenesis testis topical drug administration |
| description |
We evaluated the 'in vitro' effect of a selective peripheral benodiazepine (BZD) receptor agonist, Ro 5-4864, on basal and hCG-stimulated androgen production by testicular interstitial cell suspensions. Ro 5-4864 (10-9-10-5 M) induced a significant increment of basal testosterone release into the medium. In addition, under conditions of hCG stimulation, Ro 5-4864 (10-7 M) induced a potentiated response to the gonadotropin in a dose-dependent manner. The selective peripheral BZD antagonist PK 11195 fully prevented the stimulatory effect of Ro 5-4864. On the other hand, clonazepam, a central BZD agonist, failed to affect androgen production significantly, whereas diazepam (10-5-10-4 M), which binds to both central and peripheral BZD receptors, was able to induce a significant increment of basal and hCG-stimulated testosterone production. These results suggest that under our experimental conditions Ro 5-4864 exerts an effect on testicular steroidogenesis, presumably through binding to the previously described peripheral-type BZD receptor. |
| format |
JOUR |
| author |
Ritta, M.N. Calandra, R.S. |
| author_facet |
Ritta, M.N. Calandra, R.S. |
| author_sort |
Ritta, M.N. |
| title |
Testicular interstitial cells as targets for peripheral benzodiazepines |
| title_short |
Testicular interstitial cells as targets for peripheral benzodiazepines |
| title_full |
Testicular interstitial cells as targets for peripheral benzodiazepines |
| title_fullStr |
Testicular interstitial cells as targets for peripheral benzodiazepines |
| title_full_unstemmed |
Testicular interstitial cells as targets for peripheral benzodiazepines |
| title_sort |
testicular interstitial cells as targets for peripheral benzodiazepines |
| url |
http://hdl.handle.net/20.500.12110/paper_00283835_v49_n3_p262_Ritta |
| work_keys_str_mv |
AT rittamn testicularinterstitialcellsastargetsforperipheralbenzodiazepines AT calandrars testicularinterstitialcellsastargetsforperipheralbenzodiazepines |
| _version_ |
1807319161628524544 |