Melatonin inhibits β-adrenoceptor-stimulated cyclic AMP accumulation in rat astroglial cell cultures

We investigated whether astroglial cells are a site of action for the effect of melatonin on brain cyclic AMP content. Rat astroglial cell subcultures, identified according to morphological and immunochemical criteria, were used. Addition of melatonin to the cultures did not result in changes of cyc...

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Autores principales:	Vacas, M.I., Berria, M.I., Cardinali, D.P., Lascano, E.F.
Formato:	JOUR
Materias:	5 methoxytryptophol 6 chloromelatonin 6 hydroxymelatonin beta adrenergic receptor cyclic AMP isoprenaline melatonin noradrenalin serotonin animal cell astrocyte drug efficacy nervous system nonhuman rat
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00283835_v38_n3_p176_Vacas
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00283835_v38_n3_p176_Vacas
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spelling	todo:paper_00283835_v38_n3_p176_Vacas2023-10-03T14:38:49Z Melatonin inhibits β-adrenoceptor-stimulated cyclic AMP accumulation in rat astroglial cell cultures Vacas, M.I. Berria, M.I. Cardinali, D.P. Lascano, E.F. 5 methoxytryptophol 6 chloromelatonin 6 hydroxymelatonin beta adrenergic receptor cyclic AMP isoprenaline melatonin noradrenalin serotonin animal cell astrocyte drug efficacy nervous system nonhuman rat We investigated whether astroglial cells are a site of action for the effect of melatonin on brain cyclic AMP content. Rat astroglial cell subcultures, identified according to morphological and immunochemical criteria, were used. Addition of melatonin to the cultures did not result in changes of cyclic AMP content. However, melatonin at 0.1-1 μM concentrations was able to impair the cyclic AMP increase elicited by 1 μM norepinephrine or isoproterenol in astroglial cultures. This melatonin effect was also shared by its biologically active analogues 5-methoxytryptophol and 6-chloromelatonin. Serotonin was only effective at a 100-fold greater concentration, while the biologically inactive melatonin metabolite 6-hydroxymelatonin was devoid of activity at any concentration used. These results suggest that methoxyindoles modulate negatively β-adrenoceptor-induced cyclic AMP accumulation in cultured rat astroglial cells. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00283835_v38_n3_p176_Vacas
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	5 methoxytryptophol 6 chloromelatonin 6 hydroxymelatonin beta adrenergic receptor cyclic AMP isoprenaline melatonin noradrenalin serotonin animal cell astrocyte drug efficacy nervous system nonhuman rat
spellingShingle	5 methoxytryptophol 6 chloromelatonin 6 hydroxymelatonin beta adrenergic receptor cyclic AMP isoprenaline melatonin noradrenalin serotonin animal cell astrocyte drug efficacy nervous system nonhuman rat Vacas, M.I. Berria, M.I. Cardinali, D.P. Lascano, E.F. Melatonin inhibits β-adrenoceptor-stimulated cyclic AMP accumulation in rat astroglial cell cultures
topic_facet	5 methoxytryptophol 6 chloromelatonin 6 hydroxymelatonin beta adrenergic receptor cyclic AMP isoprenaline melatonin noradrenalin serotonin animal cell astrocyte drug efficacy nervous system nonhuman rat
description	We investigated whether astroglial cells are a site of action for the effect of melatonin on brain cyclic AMP content. Rat astroglial cell subcultures, identified according to morphological and immunochemical criteria, were used. Addition of melatonin to the cultures did not result in changes of cyclic AMP content. However, melatonin at 0.1-1 μM concentrations was able to impair the cyclic AMP increase elicited by 1 μM norepinephrine or isoproterenol in astroglial cultures. This melatonin effect was also shared by its biologically active analogues 5-methoxytryptophol and 6-chloromelatonin. Serotonin was only effective at a 100-fold greater concentration, while the biologically inactive melatonin metabolite 6-hydroxymelatonin was devoid of activity at any concentration used. These results suggest that methoxyindoles modulate negatively β-adrenoceptor-induced cyclic AMP accumulation in cultured rat astroglial cells.
format	JOUR
author	Vacas, M.I. Berria, M.I. Cardinali, D.P. Lascano, E.F.
author_facet	Vacas, M.I. Berria, M.I. Cardinali, D.P. Lascano, E.F.
author_sort	Vacas, M.I.
title	Melatonin inhibits β-adrenoceptor-stimulated cyclic AMP accumulation in rat astroglial cell cultures
title_short	Melatonin inhibits β-adrenoceptor-stimulated cyclic AMP accumulation in rat astroglial cell cultures
title_full	Melatonin inhibits β-adrenoceptor-stimulated cyclic AMP accumulation in rat astroglial cell cultures
title_fullStr	Melatonin inhibits β-adrenoceptor-stimulated cyclic AMP accumulation in rat astroglial cell cultures
title_full_unstemmed	Melatonin inhibits β-adrenoceptor-stimulated cyclic AMP accumulation in rat astroglial cell cultures
title_sort	melatonin inhibits β-adrenoceptor-stimulated cyclic amp accumulation in rat astroglial cell cultures
url	http://hdl.handle.net/20.500.12110/paper_00283835_v38_n3_p176_Vacas
work_keys_str_mv	AT vacasmi melatonininhibitsbadrenoceptorstimulatedcyclicampaccumulationinratastroglialcellcultures AT berriami melatonininhibitsbadrenoceptorstimulatedcyclicampaccumulationinratastroglialcellcultures AT cardinalidp melatonininhibitsbadrenoceptorstimulatedcyclicampaccumulationinratastroglialcellcultures AT lascanoef melatonininhibitsbadrenoceptorstimulatedcyclicampaccumulationinratastroglialcellcultures
_version_	1807318041034227712

Melatonin inhibits β-adrenoceptor-stimulated cyclic AMP accumulation in rat astroglial cell cultures

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