Rewiring of auxin signaling under persistent shade

Light cues from neighboring vegetation rapidly initiate plant shade-avoidance responses. Despite our detailed knowledge of the early steps of this response, the molecular events under prolonged shade are largely unclear. Here we show that persistent neighbor cues reinforce growth responses in additi...

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Autores principales: Pucciariello, O., Legris, M., Rojas, C.C., Iglesias, M.J., Hernando, C.E., Dezar, C., Vazquez, M., Yanovsky, M.J., Finlayson, S.A., Prat, S., Casal, J.J.
Formato: JOUR
Materias:
Aux/IAA
Auxin receptor
MIR393
Phytochrome B
PIF4
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00278424_v115_n21_p5612_Pucciariello
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00278424_v115_n21_p5612_Pucciariello
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spelling todo:paper_00278424_v115_n21_p5612_Pucciariello2023-10-03T14:38:16Z Rewiring of auxin signaling under persistent shade Pucciariello, O. Legris, M. Rojas, C.C. Iglesias, M.J. Hernando, C.E. Dezar, C. Vazquez, M. Yanovsky, M.J. Finlayson, S.A. Prat, S. Casal, J.J. Aux/IAA Auxin receptor MIR393 Phytochrome B PIF4 Light cues from neighboring vegetation rapidly initiate plant shade-avoidance responses. Despite our detailed knowledge of the early steps of this response, the molecular events under prolonged shade are largely unclear. Here we show that persistent neighbor cues reinforce growth responses in addition to promoting auxin-responsive gene expression in Arabidopsis and soybean. However, while the elevation of auxin levels is well established as an early event, in Arabidopsis, the response to prolonged shade occurs when auxin levels have declined to the pres-timulation values. Remarkably, the sustained low activity of phytochrome B under prolonged shade led to (i) decreased levels of PHYTOCHROME INTERACTING FACTOR 4 (PIF4) in the cotyledons (the organs that supply auxin) along with increased levels in the vascular tissues of the stem, (ii) elevated expression of the PIF4 targets INDOLE-3-ACETIC ACID 19 (IAA19) and IAA29, which in turn reduced the expression of the growth-repressive IAA17 regulator, (iii) reduced abundance of AUXIN RESPONSE FACTOR 6, (iv) reduced expression of MIR393 and increased abundance of its targets, the auxin receptors, and (v) elevated auxin signaling as indicated by molecular markers. Mathematical and genetic analyses support the physiological role of this system-level rearrangement. We propose that prolonged shade rewires the connectivity between light and auxin signaling to sustain shade avoidance without enhanced auxin levels. © 2018 National Academy of Sciences. All Rights Reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00278424_v115_n21_p5612_Pucciariello
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Aux/IAA
Auxin receptor
MIR393
Phytochrome B
PIF4
spellingShingle Aux/IAA
Auxin receptor
MIR393
Phytochrome B
PIF4
Pucciariello, O.
Legris, M.
Rojas, C.C.
Iglesias, M.J.
Hernando, C.E.
Dezar, C.
Vazquez, M.
Yanovsky, M.J.
Finlayson, S.A.
Prat, S.
Casal, J.J.
Rewiring of auxin signaling under persistent shade
topic_facet Aux/IAA
Auxin receptor
MIR393
Phytochrome B
PIF4
description Light cues from neighboring vegetation rapidly initiate plant shade-avoidance responses. Despite our detailed knowledge of the early steps of this response, the molecular events under prolonged shade are largely unclear. Here we show that persistent neighbor cues reinforce growth responses in addition to promoting auxin-responsive gene expression in Arabidopsis and soybean. However, while the elevation of auxin levels is well established as an early event, in Arabidopsis, the response to prolonged shade occurs when auxin levels have declined to the pres-timulation values. Remarkably, the sustained low activity of phytochrome B under prolonged shade led to (i) decreased levels of PHYTOCHROME INTERACTING FACTOR 4 (PIF4) in the cotyledons (the organs that supply auxin) along with increased levels in the vascular tissues of the stem, (ii) elevated expression of the PIF4 targets INDOLE-3-ACETIC ACID 19 (IAA19) and IAA29, which in turn reduced the expression of the growth-repressive IAA17 regulator, (iii) reduced abundance of AUXIN RESPONSE FACTOR 6, (iv) reduced expression of MIR393 and increased abundance of its targets, the auxin receptors, and (v) elevated auxin signaling as indicated by molecular markers. Mathematical and genetic analyses support the physiological role of this system-level rearrangement. We propose that prolonged shade rewires the connectivity between light and auxin signaling to sustain shade avoidance without enhanced auxin levels. © 2018 National Academy of Sciences. All Rights Reserved.
format JOUR
author Pucciariello, O.
Legris, M.
Rojas, C.C.
Iglesias, M.J.
Hernando, C.E.
Dezar, C.
Vazquez, M.
Yanovsky, M.J.
Finlayson, S.A.
Prat, S.
Casal, J.J.
author_facet Pucciariello, O.
Legris, M.
Rojas, C.C.
Iglesias, M.J.
Hernando, C.E.
Dezar, C.
Vazquez, M.
Yanovsky, M.J.
Finlayson, S.A.
Prat, S.
Casal, J.J.
author_sort Pucciariello, O.
title Rewiring of auxin signaling under persistent shade
title_short Rewiring of auxin signaling under persistent shade
title_full Rewiring of auxin signaling under persistent shade
title_fullStr Rewiring of auxin signaling under persistent shade
title_full_unstemmed Rewiring of auxin signaling under persistent shade
title_sort rewiring of auxin signaling under persistent shade
url http://hdl.handle.net/20.500.12110/paper_00278424_v115_n21_p5612_Pucciariello
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