Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3
The role of electrical activity in axon guidance has been extensively studied in vitro. To better understand its role in the intact nervous system, we imaged intracellular Ca2+ in zebrafish primary motor neurons (PMN) during axon pathfinding in vivo. We found that PMN generate specific patterns of C...
Guardado en:
| Autores principales: | , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00278424_v110_n4_p1524_Plazas |
| Aporte de: |
| id |
todo:paper_00278424_v110_n4_p1524_Plazas |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_00278424_v110_n4_p1524_Plazas2023-10-03T14:38:10Z Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3 Plazas, P.V. Nicol, X. Spitzer, N.C. inwardly rectifying potassium channel subunit Kir2.1 plexin plexin A3 unclassified drug animal cell animal tissue article calcium cell level developmental stage electric activity embryo gene silencing in vivo study motoneuron nerve fiber nerve fiber growth nonhuman perikaryon primary motor cortex priority journal protein expression regulatory mechanism signal transduction spike stereotypy synaptic transmission zebra fish Animals Animals, Genetically Modified Axons Calcium Signaling Gene Knockdown Techniques Humans Motor Neurons Neural Pathways Potassium Channels, Inwardly Rectifying Receptors, Cell Surface Recombinant Proteins Synaptic Transmission Zebrafish Zebrafish Proteins Danio rerio The role of electrical activity in axon guidance has been extensively studied in vitro. To better understand its role in the intact nervous system, we imaged intracellular Ca2+ in zebrafish primary motor neurons (PMN) during axon pathfinding in vivo. We found that PMN generate specific patterns of Ca2+ spikes at different developmental stages. Spikes arose in the distal axon of PMN and were propagated to the cell body. Suppression of Ca 2+ spiking activity in single PMN led to stereotyped errors, but silencing all electrical activity had no effect on axon guidance, indicating that an activitybased competition rule regulates this process. This competition was not mediated by synaptic transmission. Combination of PlexinA3 knockdown with suppression of Ca2+ activity in single PMN produced a synergistic increase in the incidence of pathfinding errors. However, expression of PlexinA3 transcripts was not regulated by activity. Our results provide an in vivo demonstration of the intersection of spontaneous electrical activity with the PlexinA3 guidance molecule receptor in regulation of axon pathfinding. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00278424_v110_n4_p1524_Plazas |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
inwardly rectifying potassium channel subunit Kir2.1 plexin plexin A3 unclassified drug animal cell animal tissue article calcium cell level developmental stage electric activity embryo gene silencing in vivo study motoneuron nerve fiber nerve fiber growth nonhuman perikaryon primary motor cortex priority journal protein expression regulatory mechanism signal transduction spike stereotypy synaptic transmission zebra fish Animals Animals, Genetically Modified Axons Calcium Signaling Gene Knockdown Techniques Humans Motor Neurons Neural Pathways Potassium Channels, Inwardly Rectifying Receptors, Cell Surface Recombinant Proteins Synaptic Transmission Zebrafish Zebrafish Proteins Danio rerio |
| spellingShingle |
inwardly rectifying potassium channel subunit Kir2.1 plexin plexin A3 unclassified drug animal cell animal tissue article calcium cell level developmental stage electric activity embryo gene silencing in vivo study motoneuron nerve fiber nerve fiber growth nonhuman perikaryon primary motor cortex priority journal protein expression regulatory mechanism signal transduction spike stereotypy synaptic transmission zebra fish Animals Animals, Genetically Modified Axons Calcium Signaling Gene Knockdown Techniques Humans Motor Neurons Neural Pathways Potassium Channels, Inwardly Rectifying Receptors, Cell Surface Recombinant Proteins Synaptic Transmission Zebrafish Zebrafish Proteins Danio rerio Plazas, P.V. Nicol, X. Spitzer, N.C. Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3 |
| topic_facet |
inwardly rectifying potassium channel subunit Kir2.1 plexin plexin A3 unclassified drug animal cell animal tissue article calcium cell level developmental stage electric activity embryo gene silencing in vivo study motoneuron nerve fiber nerve fiber growth nonhuman perikaryon primary motor cortex priority journal protein expression regulatory mechanism signal transduction spike stereotypy synaptic transmission zebra fish Animals Animals, Genetically Modified Axons Calcium Signaling Gene Knockdown Techniques Humans Motor Neurons Neural Pathways Potassium Channels, Inwardly Rectifying Receptors, Cell Surface Recombinant Proteins Synaptic Transmission Zebrafish Zebrafish Proteins Danio rerio |
| description |
The role of electrical activity in axon guidance has been extensively studied in vitro. To better understand its role in the intact nervous system, we imaged intracellular Ca2+ in zebrafish primary motor neurons (PMN) during axon pathfinding in vivo. We found that PMN generate specific patterns of Ca2+ spikes at different developmental stages. Spikes arose in the distal axon of PMN and were propagated to the cell body. Suppression of Ca 2+ spiking activity in single PMN led to stereotyped errors, but silencing all electrical activity had no effect on axon guidance, indicating that an activitybased competition rule regulates this process. This competition was not mediated by synaptic transmission. Combination of PlexinA3 knockdown with suppression of Ca2+ activity in single PMN produced a synergistic increase in the incidence of pathfinding errors. However, expression of PlexinA3 transcripts was not regulated by activity. Our results provide an in vivo demonstration of the intersection of spontaneous electrical activity with the PlexinA3 guidance molecule receptor in regulation of axon pathfinding. |
| format |
JOUR |
| author |
Plazas, P.V. Nicol, X. Spitzer, N.C. |
| author_facet |
Plazas, P.V. Nicol, X. Spitzer, N.C. |
| author_sort |
Plazas, P.V. |
| title |
Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3 |
| title_short |
Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3 |
| title_full |
Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3 |
| title_fullStr |
Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3 |
| title_full_unstemmed |
Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3 |
| title_sort |
activity-dependent competition regulates motor neuron axon pathfinding via plexina3 |
| url |
http://hdl.handle.net/20.500.12110/paper_00278424_v110_n4_p1524_Plazas |
| work_keys_str_mv |
AT plazaspv activitydependentcompetitionregulatesmotorneuronaxonpathfindingviaplexina3 AT nicolx activitydependentcompetitionregulatesmotorneuronaxonpathfindingviaplexina3 AT spitzernc activitydependentcompetitionregulatesmotorneuronaxonpathfindingviaplexina3 |
| _version_ |
1807324232190787584 |