Factors influencing cellulase production Saccobolus saccoboloides

Saccobolus saccoboloides was grown in a liquid synthetic medium with crystalline cellulose as the only carbon source. Culture supernatant was used to measure activities of the enzymes of the cellulolytic complex: β-1,4 endoglucanase, β-1,4 exoglucanase and β-glucosidase. The kinetics of growth and e...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Magnelli, P., Ramos, A.M., Forchiassin, F.
Formato: JOUR
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00275514_v88_n2_p249_Magnelli
Aporte de:
id todo:paper_00275514_v88_n2_p249_Magnelli
record_format dspace
spelling todo:paper_00275514_v88_n2_p249_Magnelli2023-10-03T14:37:40Z Factors influencing cellulase production Saccobolus saccoboloides Magnelli, P. Ramos, A.M. Forchiassin, F. Ascobolaceae Endoglucanase Nutrition β-glucosidase Ascobolaceae Saccobolus Saccobolus saccoboloides Saccobolus saccoboloides was grown in a liquid synthetic medium with crystalline cellulose as the only carbon source. Culture supernatant was used to measure activities of the enzymes of the cellulolytic complex: β-1,4 endoglucanase, β-1,4 exoglucanase and β-glucosidase. The kinetics of growth and enzyme production were studied in static and shaken cultures. The influence of various nitrogen sources, on production of the various cellulases in the culture medium was investigated. Growth and cellulolytic enzymes production were maximal when organic nitrogenous compounds were supplied. Enzymatic production was maximal at an initial pH of the growth medium of 6.5. Enzymatic activities were favored in the range of 50-55 C and pH 4.8-5.4. Fil:Magnelli, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ramos, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Forchiassin, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00275514_v88_n2_p249_Magnelli
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Ascobolaceae
Endoglucanase
Nutrition
β-glucosidase
Ascobolaceae
Saccobolus
Saccobolus saccoboloides
spellingShingle Ascobolaceae
Endoglucanase
Nutrition
β-glucosidase
Ascobolaceae
Saccobolus
Saccobolus saccoboloides
Magnelli, P.
Ramos, A.M.
Forchiassin, F.
Factors influencing cellulase production Saccobolus saccoboloides
topic_facet Ascobolaceae
Endoglucanase
Nutrition
β-glucosidase
Ascobolaceae
Saccobolus
Saccobolus saccoboloides
description Saccobolus saccoboloides was grown in a liquid synthetic medium with crystalline cellulose as the only carbon source. Culture supernatant was used to measure activities of the enzymes of the cellulolytic complex: β-1,4 endoglucanase, β-1,4 exoglucanase and β-glucosidase. The kinetics of growth and enzyme production were studied in static and shaken cultures. The influence of various nitrogen sources, on production of the various cellulases in the culture medium was investigated. Growth and cellulolytic enzymes production were maximal when organic nitrogenous compounds were supplied. Enzymatic production was maximal at an initial pH of the growth medium of 6.5. Enzymatic activities were favored in the range of 50-55 C and pH 4.8-5.4.
format JOUR
author Magnelli, P.
Ramos, A.M.
Forchiassin, F.
author_facet Magnelli, P.
Ramos, A.M.
Forchiassin, F.
author_sort Magnelli, P.
title Factors influencing cellulase production Saccobolus saccoboloides
title_short Factors influencing cellulase production Saccobolus saccoboloides
title_full Factors influencing cellulase production Saccobolus saccoboloides
title_fullStr Factors influencing cellulase production Saccobolus saccoboloides
title_full_unstemmed Factors influencing cellulase production Saccobolus saccoboloides
title_sort factors influencing cellulase production saccobolus saccoboloides
url http://hdl.handle.net/20.500.12110/paper_00275514_v88_n2_p249_Magnelli
work_keys_str_mv AT magnellip factorsinfluencingcellulaseproductionsaccobolussaccoboloides
AT ramosam factorsinfluencingcellulaseproductionsaccobolussaccoboloides
AT forchiassinf factorsinfluencingcellulaseproductionsaccobolussaccoboloides
_version_ 1807319779756736512