Phylogenetic divergence, morphological and physiological differences distinguish a new Neotyphodium endophyte species in the grass Bromus auleticus from South America
The fungi of genus Neotyphodium are systemic, constitutive, symbionts of grasses of subfamily Pooideae. In the southern hemisphere most of these asexual endophytes are the result of the hybridization between two sexual species, Epichloe festucae and E. typhina, from the northern hemisphere. However...
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todo:paper_00275514_v101_n3_p340_Iannone2023-10-03T14:37:33Z Phylogenetic divergence, morphological and physiological differences distinguish a new Neotyphodium endophyte species in the grass Bromus auleticus from South America Iannone, L.J. Cabral, D. Schardl, C.L. Rossi, M.S. Bromus auleticus Endophyte diversity Molecular phylogeny Neotyphodium divergence endophyte fungicide fungus genotype grass growth rate hybridization morphology new species ontogeny pesticide resistance phylogenetics physiology species diversity South America Bromus Epichloe festucae Fungi Neotyphodium Poa Poaceae Pooideae elongation factor 1 fungal DNA ribosomal spacer DNA tubulin Argentina article Bromus classification cytology DNA sequence ecosystem fungus spore genetic variability genetics isolation and purification microbiology Neotyphodium phylogeny physiology species difference species differentiation symbiosis Argentina Bromus DNA, Fungal DNA, Ribosomal Spacer Ecosystem Genetic Speciation Genetic Variation Neotyphodium Peptide Elongation Factor 1 Phylogeny Sequence Analysis, DNA Species Specificity Spores, Fungal Symbiosis Tubulin The fungi of genus Neotyphodium are systemic, constitutive, symbionts of grasses of subfamily Pooideae. In the southern hemisphere most of these asexual endophytes are the result of the hybridization between two sexual species, Epichloe festucae and E. typhina, from the northern hemisphere. However the ancestral sexual species have not been detected in this region. Several grasses from Argentina are infected by Neotyphodium species. These endophytes are in general very similar macro-and micromorphologically and phylogenetically conform to species N tembladerae. However the Neotyphodium spp. endophytes of some hosts, Bromus auleticus and Poa spicifomis var. spiciformis, have not been included in this species. In this work we studied the incidence and characterized the diversity of Neotyphodium species in populations of the native grass Bromus auleticus from Argentina. The incidence of endophytes was 100% in all populations investigated. Two groups of endophytes were differentiated by their morphologies, growth rates, conidial ontogenies and by relative resistance to the fungicide benomyl. Phylogenetic trees inferred from tefA and tubB intron sequences indicated that both N tembladerae and the novel morphotype were hybrids of E. festucae and E. typhina, but the ancestral E. typhina genotype distinguished them. Isolates from plants that inhabit coastal dunes, xerophytic forests, savannahs and hills were similar morphologically and phylogenetically to N tembladerae, whereas the endophytes from the humid pampa plains conformed to the novel group. We propose the endophyte of Bromus auleticus from humid pampas as a new species, Neotyphodium pampeanum. © 2009 by The Mycological Society of America. Fil:Iannone, L.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cabral, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rossi, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00275514_v101_n3_p340_Iannone |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bromus auleticus Endophyte diversity Molecular phylogeny Neotyphodium divergence endophyte fungicide fungus genotype grass growth rate hybridization morphology new species ontogeny pesticide resistance phylogenetics physiology species diversity South America Bromus Epichloe festucae Fungi Neotyphodium Poa Poaceae Pooideae elongation factor 1 fungal DNA ribosomal spacer DNA tubulin Argentina article Bromus classification cytology DNA sequence ecosystem fungus spore genetic variability genetics isolation and purification microbiology Neotyphodium phylogeny physiology species difference species differentiation symbiosis Argentina Bromus DNA, Fungal DNA, Ribosomal Spacer Ecosystem Genetic Speciation Genetic Variation Neotyphodium Peptide Elongation Factor 1 Phylogeny Sequence Analysis, DNA Species Specificity Spores, Fungal Symbiosis Tubulin |
| spellingShingle |
Bromus auleticus Endophyte diversity Molecular phylogeny Neotyphodium divergence endophyte fungicide fungus genotype grass growth rate hybridization morphology new species ontogeny pesticide resistance phylogenetics physiology species diversity South America Bromus Epichloe festucae Fungi Neotyphodium Poa Poaceae Pooideae elongation factor 1 fungal DNA ribosomal spacer DNA tubulin Argentina article Bromus classification cytology DNA sequence ecosystem fungus spore genetic variability genetics isolation and purification microbiology Neotyphodium phylogeny physiology species difference species differentiation symbiosis Argentina Bromus DNA, Fungal DNA, Ribosomal Spacer Ecosystem Genetic Speciation Genetic Variation Neotyphodium Peptide Elongation Factor 1 Phylogeny Sequence Analysis, DNA Species Specificity Spores, Fungal Symbiosis Tubulin Iannone, L.J. Cabral, D. Schardl, C.L. Rossi, M.S. Phylogenetic divergence, morphological and physiological differences distinguish a new Neotyphodium endophyte species in the grass Bromus auleticus from South America |
| topic_facet |
Bromus auleticus Endophyte diversity Molecular phylogeny Neotyphodium divergence endophyte fungicide fungus genotype grass growth rate hybridization morphology new species ontogeny pesticide resistance phylogenetics physiology species diversity South America Bromus Epichloe festucae Fungi Neotyphodium Poa Poaceae Pooideae elongation factor 1 fungal DNA ribosomal spacer DNA tubulin Argentina article Bromus classification cytology DNA sequence ecosystem fungus spore genetic variability genetics isolation and purification microbiology Neotyphodium phylogeny physiology species difference species differentiation symbiosis Argentina Bromus DNA, Fungal DNA, Ribosomal Spacer Ecosystem Genetic Speciation Genetic Variation Neotyphodium Peptide Elongation Factor 1 Phylogeny Sequence Analysis, DNA Species Specificity Spores, Fungal Symbiosis Tubulin |
| description |
The fungi of genus Neotyphodium are systemic, constitutive, symbionts of grasses of subfamily Pooideae. In the southern hemisphere most of these asexual endophytes are the result of the hybridization between two sexual species, Epichloe festucae and E. typhina, from the northern hemisphere. However the ancestral sexual species have not been detected in this region. Several grasses from Argentina are infected by Neotyphodium species. These endophytes are in general very similar macro-and micromorphologically and phylogenetically conform to species N tembladerae. However the Neotyphodium spp. endophytes of some hosts, Bromus auleticus and Poa spicifomis var. spiciformis, have not been included in this species. In this work we studied the incidence and characterized the diversity of Neotyphodium species in populations of the native grass Bromus auleticus from Argentina. The incidence of endophytes was 100% in all populations investigated. Two groups of endophytes were differentiated by their morphologies, growth rates, conidial ontogenies and by relative resistance to the fungicide benomyl. Phylogenetic trees inferred from tefA and tubB intron sequences indicated that both N tembladerae and the novel morphotype were hybrids of E. festucae and E. typhina, but the ancestral E. typhina genotype distinguished them. Isolates from plants that inhabit coastal dunes, xerophytic forests, savannahs and hills were similar morphologically and phylogenetically to N tembladerae, whereas the endophytes from the humid pampa plains conformed to the novel group. We propose the endophyte of Bromus auleticus from humid pampas as a new species, Neotyphodium pampeanum. © 2009 by The Mycological Society of America. |
| format |
JOUR |
| author |
Iannone, L.J. Cabral, D. Schardl, C.L. Rossi, M.S. |
| author_facet |
Iannone, L.J. Cabral, D. Schardl, C.L. Rossi, M.S. |
| author_sort |
Iannone, L.J. |
| title |
Phylogenetic divergence, morphological and physiological differences distinguish a new Neotyphodium endophyte species in the grass Bromus auleticus from South America |
| title_short |
Phylogenetic divergence, morphological and physiological differences distinguish a new Neotyphodium endophyte species in the grass Bromus auleticus from South America |
| title_full |
Phylogenetic divergence, morphological and physiological differences distinguish a new Neotyphodium endophyte species in the grass Bromus auleticus from South America |
| title_fullStr |
Phylogenetic divergence, morphological and physiological differences distinguish a new Neotyphodium endophyte species in the grass Bromus auleticus from South America |
| title_full_unstemmed |
Phylogenetic divergence, morphological and physiological differences distinguish a new Neotyphodium endophyte species in the grass Bromus auleticus from South America |
| title_sort |
phylogenetic divergence, morphological and physiological differences distinguish a new neotyphodium endophyte species in the grass bromus auleticus from south america |
| url |
http://hdl.handle.net/20.500.12110/paper_00275514_v101_n3_p340_Iannone |
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