Analysis of an abscisic acid (ABA)-responsive gene promoter belonging to the Asr gene family from tomato in homologous and heterologous systems

Asr is a family of genes that maps to chromosome 4 of tomato. Asr2, a recently reported member of this family, is believed to be regulated by abscisic acid (ABA), stress and ripening. A genomic Asr2 clone has been fully sequenced, and candidate upstream regulatory elements have been identified. To p...

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Autores principales: Rossi, M., Carrari, F., Cabrera-Ponce, J.L., Vázquez-Rovere, C., Herrera-Estrella, L., Gudesblat, G., Iusem, N.D.
Formato: JOUR
Materias:
Abscisic acid (ABA)
Asr genes
Asr2
Drought stress
Tomato
abscisic acid
beta glucuronidase
article
gene expression
gene fusion
multigene family
nonhuman
priority journal
promoter region
reporter gene
tomato
transcription regulation
Carica papaya
Embryophyta
Lycopersicon esculentum
Nicotiana tabacum
Solanum tuberosum
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00268925_v258_n1-2_p1_Rossi
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00268925_v258_n1-2_p1_Rossi
record_format dspace
spelling todo:paper_00268925_v258_n1-2_p1_Rossi2023-10-03T14:37:09Z Analysis of an abscisic acid (ABA)-responsive gene promoter belonging to the Asr gene family from tomato in homologous and heterologous systems Rossi, M. Carrari, F. Cabrera-Ponce, J.L. Vázquez-Rovere, C. Herrera-Estrella, L. Gudesblat, G. Iusem, N.D. Abscisic acid (ABA) Asr genes Asr2 Drought stress Tomato abscisic acid beta glucuronidase article gene expression gene fusion multigene family nonhuman priority journal promoter region reporter gene tomato transcription regulation Carica papaya Embryophyta Lycopersicon esculentum Nicotiana tabacum Solanum tuberosum Asr is a family of genes that maps to chromosome 4 of tomato. Asr2, a recently reported member of this family, is believed to be regulated by abscisic acid (ABA), stress and ripening. A genomic Asr2 clone has been fully sequenced, and candidate upstream regulatory elements have been identified. To prove that the promoter region is functional in vivo, we fused it upstream of the β-glucuronidase (GUS) reporter gene. The resulting chimeric gene fusion was used for transient expression assays in papaya embryogenic calli and leaves. In addition, the same construct was used to produce transgenic tomato, papaya, tobacco, and potato plants. Asr2 upstream sequences showed promoter function in all of these systems. Under the experimental conditions tested, ABA stimulated GUS expression in papaya and tobacco, but not in tomato and potato systems. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00268925_v258_n1-2_p1_Rossi
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Abscisic acid (ABA)
Asr genes
Asr2
Drought stress
Tomato
abscisic acid
beta glucuronidase
article
gene expression
gene fusion
multigene family
nonhuman
priority journal
promoter region
reporter gene
tomato
transcription regulation
Carica papaya
Embryophyta
Lycopersicon esculentum
Nicotiana tabacum
Solanum tuberosum
spellingShingle Abscisic acid (ABA)
Asr genes
Asr2
Drought stress
Tomato
abscisic acid
beta glucuronidase
article
gene expression
gene fusion
multigene family
nonhuman
priority journal
promoter region
reporter gene
tomato
transcription regulation
Carica papaya
Embryophyta
Lycopersicon esculentum
Nicotiana tabacum
Solanum tuberosum
Rossi, M.
Carrari, F.
Cabrera-Ponce, J.L.
Vázquez-Rovere, C.
Herrera-Estrella, L.
Gudesblat, G.
Iusem, N.D.
Analysis of an abscisic acid (ABA)-responsive gene promoter belonging to the Asr gene family from tomato in homologous and heterologous systems
topic_facet Abscisic acid (ABA)
Asr genes
Asr2
Drought stress
Tomato
abscisic acid
beta glucuronidase
article
gene expression
gene fusion
multigene family
nonhuman
priority journal
promoter region
reporter gene
tomato
transcription regulation
Carica papaya
Embryophyta
Lycopersicon esculentum
Nicotiana tabacum
Solanum tuberosum
description Asr is a family of genes that maps to chromosome 4 of tomato. Asr2, a recently reported member of this family, is believed to be regulated by abscisic acid (ABA), stress and ripening. A genomic Asr2 clone has been fully sequenced, and candidate upstream regulatory elements have been identified. To prove that the promoter region is functional in vivo, we fused it upstream of the β-glucuronidase (GUS) reporter gene. The resulting chimeric gene fusion was used for transient expression assays in papaya embryogenic calli and leaves. In addition, the same construct was used to produce transgenic tomato, papaya, tobacco, and potato plants. Asr2 upstream sequences showed promoter function in all of these systems. Under the experimental conditions tested, ABA stimulated GUS expression in papaya and tobacco, but not in tomato and potato systems.
format JOUR
author Rossi, M.
Carrari, F.
Cabrera-Ponce, J.L.
Vázquez-Rovere, C.
Herrera-Estrella, L.
Gudesblat, G.
Iusem, N.D.
author_facet Rossi, M.
Carrari, F.
Cabrera-Ponce, J.L.
Vázquez-Rovere, C.
Herrera-Estrella, L.
Gudesblat, G.
Iusem, N.D.
author_sort Rossi, M.
title Analysis of an abscisic acid (ABA)-responsive gene promoter belonging to the Asr gene family from tomato in homologous and heterologous systems
title_short Analysis of an abscisic acid (ABA)-responsive gene promoter belonging to the Asr gene family from tomato in homologous and heterologous systems
title_full Analysis of an abscisic acid (ABA)-responsive gene promoter belonging to the Asr gene family from tomato in homologous and heterologous systems
title_fullStr Analysis of an abscisic acid (ABA)-responsive gene promoter belonging to the Asr gene family from tomato in homologous and heterologous systems
title_full_unstemmed Analysis of an abscisic acid (ABA)-responsive gene promoter belonging to the Asr gene family from tomato in homologous and heterologous systems
title_sort analysis of an abscisic acid (aba)-responsive gene promoter belonging to the asr gene family from tomato in homologous and heterologous systems
url http://hdl.handle.net/20.500.12110/paper_00268925_v258_n1-2_p1_Rossi
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