Investigation of proteins in samples of a mid-18th century colonial mural painting by MALDI-TOF/MS and LC-ESI/MS (Orbitrap)

In this paper, we report the study of proteinaceous binders in samples from a South American Colonial 18th century painting using two complementary mass spectrometry techniques. Before restoration, seven micro-samples were extracted from representative colors of one of the mural paintings located in...

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Autores principales: Levy, I.K., Neme Tauil, R., Valacco, M.P., Moreno, S., Siracusano, G., Maier, M.S.
Formato: JOUR
Materias:
Colonial mural paintings
Egg tempera technique
LC-ESI/MS (Orbitrap)
MALDI-TOF/MS
Proteinaceous binders
Proteomic tools
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0026265X_v143_n_p457_Levy
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_0026265X_v143_n_p457_Levy
record_format dspace
spelling todo:paper_0026265X_v143_n_p457_Levy2023-10-03T14:36:58Z Investigation of proteins in samples of a mid-18th century colonial mural painting by MALDI-TOF/MS and LC-ESI/MS (Orbitrap) Levy, I.K. Neme Tauil, R. Valacco, M.P. Moreno, S. Siracusano, G. Maier, M.S. Colonial mural paintings Egg tempera technique LC-ESI/MS (Orbitrap) MALDI-TOF/MS Proteinaceous binders Proteomic tools In this paper, we report the study of proteinaceous binders in samples from a South American Colonial 18th century painting using two complementary mass spectrometry techniques. Before restoration, seven micro-samples were extracted from representative colors of one of the mural paintings located inside the church of Our Lady of Copacabana de Andamarca built in 1723 in Bolivia. Previous analysis by gas chromatography (GC) of the amino acid derivatives of the extracted protein fraction of three of the samples, suggested the presence of animal glue and egg. In this work, we introduce a methodology that combines a protein extraction procedure with a typical treatment for analysis of proteins as tryptic peptides by mass spectrometry. For this study, we applied in the first stage, MALDI-TOF/MS and then, LC-ESI/MS (Orbitrap), an ultrahigh resolution mass spectrometry, to achieve more reliability in the identification of the protein binders. By LC-ESI/MS (Orbitrap), we detected several peptides from egg white proteins, particularly ovalbumin, ovotransferrin and lysozyme, and egg yolk proteins, vitellogenin-2 and apolipoprotein B, with high confidence peptides each. In all the samples, the presence of collagen from animal provenance was established by MALDI-TOF/MS and LC-ESI/MS (Orbitrap). To our knowledge, this is the first time that protein binders in Andean paintings are identified without ambiguity by MALDI-TOF/MS and LC-ESI/MS (Orbitrap) mass spectrometry using a proteomic approach. © 2018 Elsevier B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0026265X_v143_n_p457_Levy
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Colonial mural paintings
Egg tempera technique
LC-ESI/MS (Orbitrap)
MALDI-TOF/MS
Proteinaceous binders
Proteomic tools
spellingShingle Colonial mural paintings
Egg tempera technique
LC-ESI/MS (Orbitrap)
MALDI-TOF/MS
Proteinaceous binders
Proteomic tools
Levy, I.K.
Neme Tauil, R.
Valacco, M.P.
Moreno, S.
Siracusano, G.
Maier, M.S.
Investigation of proteins in samples of a mid-18th century colonial mural painting by MALDI-TOF/MS and LC-ESI/MS (Orbitrap)
topic_facet Colonial mural paintings
Egg tempera technique
LC-ESI/MS (Orbitrap)
MALDI-TOF/MS
Proteinaceous binders
Proteomic tools
description In this paper, we report the study of proteinaceous binders in samples from a South American Colonial 18th century painting using two complementary mass spectrometry techniques. Before restoration, seven micro-samples were extracted from representative colors of one of the mural paintings located inside the church of Our Lady of Copacabana de Andamarca built in 1723 in Bolivia. Previous analysis by gas chromatography (GC) of the amino acid derivatives of the extracted protein fraction of three of the samples, suggested the presence of animal glue and egg. In this work, we introduce a methodology that combines a protein extraction procedure with a typical treatment for analysis of proteins as tryptic peptides by mass spectrometry. For this study, we applied in the first stage, MALDI-TOF/MS and then, LC-ESI/MS (Orbitrap), an ultrahigh resolution mass spectrometry, to achieve more reliability in the identification of the protein binders. By LC-ESI/MS (Orbitrap), we detected several peptides from egg white proteins, particularly ovalbumin, ovotransferrin and lysozyme, and egg yolk proteins, vitellogenin-2 and apolipoprotein B, with high confidence peptides each. In all the samples, the presence of collagen from animal provenance was established by MALDI-TOF/MS and LC-ESI/MS (Orbitrap). To our knowledge, this is the first time that protein binders in Andean paintings are identified without ambiguity by MALDI-TOF/MS and LC-ESI/MS (Orbitrap) mass spectrometry using a proteomic approach. © 2018 Elsevier B.V.
format JOUR
author Levy, I.K.
Neme Tauil, R.
Valacco, M.P.
Moreno, S.
Siracusano, G.
Maier, M.S.
author_facet Levy, I.K.
Neme Tauil, R.
Valacco, M.P.
Moreno, S.
Siracusano, G.
Maier, M.S.
author_sort Levy, I.K.
title Investigation of proteins in samples of a mid-18th century colonial mural painting by MALDI-TOF/MS and LC-ESI/MS (Orbitrap)
title_short Investigation of proteins in samples of a mid-18th century colonial mural painting by MALDI-TOF/MS and LC-ESI/MS (Orbitrap)
title_full Investigation of proteins in samples of a mid-18th century colonial mural painting by MALDI-TOF/MS and LC-ESI/MS (Orbitrap)
title_fullStr Investigation of proteins in samples of a mid-18th century colonial mural painting by MALDI-TOF/MS and LC-ESI/MS (Orbitrap)
title_full_unstemmed Investigation of proteins in samples of a mid-18th century colonial mural painting by MALDI-TOF/MS and LC-ESI/MS (Orbitrap)
title_sort investigation of proteins in samples of a mid-18th century colonial mural painting by maldi-tof/ms and lc-esi/ms (orbitrap)
url http://hdl.handle.net/20.500.12110/paper_0026265X_v143_n_p457_Levy
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