The specific neuroendocrine profile is critically involved in an adequate T-dependent immune response

A functional relationship between the neuroendocrine and immune systems has been clearly established. We examined the role of neuroendocrine changes, particularly thyrotropin-releasing hormone (TRH) and prolactin (PRL), during the T cell-dependent immune response. After immunization of rats with she...

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Autores principales: Castro, C.P., Pereda, M.P., Keller, E.A., Arzt, E.
Formato: JOUR
Materias:
Immune modulation
Prolactin
SRBC
T-lymphocytes
TRH antisense
antisense oligonucleotide
protirelin
analysis of variance
animal
antibody production
article
blood
erythrocyte
immune system
immunology
male
metabolism
neurosecretion
rat
sheep
T lymphocyte
Wistar rat
Analysis of Variance
Animals
Antibody Formation
Erythrocytes
Immune System
Male
Neurosecretory Systems
Oligonucleotides, Antisense
Rats
Rats, Wistar
Sheep
T-Lymphocytes
Thyrotropin-Releasing Hormone
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00257680_v58_n2_p189_Castro
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_00257680_v58_n2_p189_Castro2023-10-03T14:36:34Z The specific neuroendocrine profile is critically involved in an adequate T-dependent immune response Castro, C.P. Pereda, M.P. Keller, E.A. Arzt, E. Immune modulation Prolactin SRBC T-lymphocytes TRH antisense antisense oligonucleotide protirelin analysis of variance animal antibody production article blood erythrocyte immune system immunology male metabolism neurosecretion rat sheep T lymphocyte Wistar rat Analysis of Variance Animals Antibody Formation Erythrocytes Immune System Male Neurosecretory Systems Oligonucleotides, Antisense Rats Rats, Wistar Sheep T-Lymphocytes Thyrotropin-Releasing Hormone A functional relationship between the neuroendocrine and immune systems has been clearly established. We examined the role of neuroendocrine changes, particularly thyrotropin-releasing hormone (TRH) and prolactin (PRL), during the T cell-dependent immune response. After immunization of rats with sheep red blood cells (SRBC, a T cell-dependent antigen) we observed: a) an increase of hypothalamic TRH mRNA at 4 to 24 h post-immunization (i.e: SRBC vs saline: 4 h, 2.8x), in contrast to the decrease of TRH mRNA observed following treatment with LPS, a T-independent antigen (LPS vs saline: 4 h, 1.6x); b) an increase in pituitary TRH receptor mRNA and plasma PRL levels but no changes in thyroid-stimulating hormone and growth hormone plasma levels. Intracerebroventricular (icv) injection in conscious freely-moving rats of antisense oligonucleotide complementary to rat TRH mRNA resulted in: a) a significant inhibition of specific antibody production [ELISA 7 days: Ig(M+G): TRH sense vs TRH-antisense: 384 ± 27 (n = 11) vs 193 ± 22 (n = 11); p < 0.001] b) an inability to produce the peak in plasma PRL levels in rats immunized with SRBC [(12h post-immunization, TRH-sense vs TRH-antisense: 8.3 ± 1.4 (n = 6) vs 2.2 ± 0.5 (n = 6); p < 0 01]; c) a decrease in hypothalamic TRH mRNA (TRH-sense vs TRH-antisense: 12h, 1.7x). These studies demonstrate that the T-cell antigen needs an early activation of TRH and PRL for an adequate immune response, in contrast to the inhibition induced by a T-cell independent antigen. Fil:Castro, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pereda, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00257680_v58_n2_p189_Castro
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Immune modulation
Prolactin
SRBC
T-lymphocytes
TRH antisense
antisense oligonucleotide
protirelin
analysis of variance
animal
antibody production
article
blood
erythrocyte
immune system
immunology
male
metabolism
neurosecretion
rat
sheep
T lymphocyte
Wistar rat
Analysis of Variance
Animals
Antibody Formation
Erythrocytes
Immune System
Male
Neurosecretory Systems
Oligonucleotides, Antisense
Rats
Rats, Wistar
Sheep
T-Lymphocytes
Thyrotropin-Releasing Hormone
spellingShingle Immune modulation
Prolactin
SRBC
T-lymphocytes
TRH antisense
antisense oligonucleotide
protirelin
analysis of variance
animal
antibody production
article
blood
erythrocyte
immune system
immunology
male
metabolism
neurosecretion
rat
sheep
T lymphocyte
Wistar rat
Analysis of Variance
Animals
Antibody Formation
Erythrocytes
Immune System
Male
Neurosecretory Systems
Oligonucleotides, Antisense
Rats
Rats, Wistar
Sheep
T-Lymphocytes
Thyrotropin-Releasing Hormone
Castro, C.P.
Pereda, M.P.
Keller, E.A.
Arzt, E.
The specific neuroendocrine profile is critically involved in an adequate T-dependent immune response
topic_facet Immune modulation
Prolactin
SRBC
T-lymphocytes
TRH antisense
antisense oligonucleotide
protirelin
analysis of variance
animal
antibody production
article
blood
erythrocyte
immune system
immunology
male
metabolism
neurosecretion
rat
sheep
T lymphocyte
Wistar rat
Analysis of Variance
Animals
Antibody Formation
Erythrocytes
Immune System
Male
Neurosecretory Systems
Oligonucleotides, Antisense
Rats
Rats, Wistar
Sheep
T-Lymphocytes
Thyrotropin-Releasing Hormone
description A functional relationship between the neuroendocrine and immune systems has been clearly established. We examined the role of neuroendocrine changes, particularly thyrotropin-releasing hormone (TRH) and prolactin (PRL), during the T cell-dependent immune response. After immunization of rats with sheep red blood cells (SRBC, a T cell-dependent antigen) we observed: a) an increase of hypothalamic TRH mRNA at 4 to 24 h post-immunization (i.e: SRBC vs saline: 4 h, 2.8x), in contrast to the decrease of TRH mRNA observed following treatment with LPS, a T-independent antigen (LPS vs saline: 4 h, 1.6x); b) an increase in pituitary TRH receptor mRNA and plasma PRL levels but no changes in thyroid-stimulating hormone and growth hormone plasma levels. Intracerebroventricular (icv) injection in conscious freely-moving rats of antisense oligonucleotide complementary to rat TRH mRNA resulted in: a) a significant inhibition of specific antibody production [ELISA 7 days: Ig(M+G): TRH sense vs TRH-antisense: 384 ± 27 (n = 11) vs 193 ± 22 (n = 11); p < 0.001] b) an inability to produce the peak in plasma PRL levels in rats immunized with SRBC [(12h post-immunization, TRH-sense vs TRH-antisense: 8.3 ± 1.4 (n = 6) vs 2.2 ± 0.5 (n = 6); p < 0 01]; c) a decrease in hypothalamic TRH mRNA (TRH-sense vs TRH-antisense: 12h, 1.7x). These studies demonstrate that the T-cell antigen needs an early activation of TRH and PRL for an adequate immune response, in contrast to the inhibition induced by a T-cell independent antigen.
format JOUR
author Castro, C.P.
Pereda, M.P.
Keller, E.A.
Arzt, E.
author_facet Castro, C.P.
Pereda, M.P.
Keller, E.A.
Arzt, E.
author_sort Castro, C.P.
title The specific neuroendocrine profile is critically involved in an adequate T-dependent immune response
title_short The specific neuroendocrine profile is critically involved in an adequate T-dependent immune response
title_full The specific neuroendocrine profile is critically involved in an adequate T-dependent immune response
title_fullStr The specific neuroendocrine profile is critically involved in an adequate T-dependent immune response
title_full_unstemmed The specific neuroendocrine profile is critically involved in an adequate T-dependent immune response
title_sort specific neuroendocrine profile is critically involved in an adequate t-dependent immune response
url http://hdl.handle.net/20.500.12110/paper_00257680_v58_n2_p189_Castro
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