Regulation of testicular steroidogenesis by nitric oxide

Testicular macrophages as well as endothelial cells, which are intimately associated with Leydig cells, constitute a potential source of paracrine nitric oxide (NO). In the present study, we investigated the effect of NO donors on MA-10 murine Leydig tumor cell line and rat Leydig cell steroidogenes...

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Autores principales:	Del Punta, K., Sanchez-Ruiz, M.E., Pignataro, O.P.
Formato:	JOUR
Materias:	cholesterol cholesterol monooxygenase (side chain cleaving) chorionic gonadotropin cyclic gmp cyclic gmp derivative hemoprotein nitric oxide nitric oxide donor pregnenolone steroid animal cell animal tissue article inhibition kinetics leydig cell macrophage activation mouse nonhuman pathophysiology rat second messenger steroidogenesis testis cell animal biosynthesis disease model Leydig cell tumor male metabolism physiology Sprague Dawley rat testis tumor Animals Disease Models, Animal Leydig Cell Tumor Male Mice Nitric Oxide Rats Rats, Sprague-Dawley Steroids Testicular Neoplasms
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00257680_v57_n3_p337_DelPunta
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00257680_v57_n3_p337_DelPunta
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spelling	todo:paper_00257680_v57_n3_p337_DelPunta2023-10-03T14:36:32Z Regulation of testicular steroidogenesis by nitric oxide Del Punta, K. Sanchez-Ruiz, M.E. Pignataro, O.P. cholesterol cholesterol monooxygenase (side chain cleaving) chorionic gonadotropin cyclic gmp cyclic gmp derivative hemoprotein nitric oxide nitric oxide donor pregnenolone nitric oxide steroid animal cell animal tissue article inhibition kinetics leydig cell macrophage activation mouse nonhuman pathophysiology rat second messenger steroidogenesis testis cell animal biosynthesis disease model Leydig cell tumor male metabolism physiology Sprague Dawley rat testis tumor Animals Disease Models, Animal Leydig Cell Tumor Male Mice Nitric Oxide Rats Rats, Sprague-Dawley Steroids Testicular Neoplasms Testicular macrophages as well as endothelial cells, which are intimately associated with Leydig cells, constitute a potential source of paracrine nitric oxide (NO). In the present study, we investigated the effect of NO donors on MA-10 murine Leydig tumor cell line and rat Leydig cell steroidogenesis. We observed that NO donors, reversibly inhibit hGG-induced steroidogenesis in both types of cells. We also studied NO mechanism of action. Contrary to what is observed in many other systems, NO inhibitory effect on Leydig cell steroidogenesis is not mediated by cGMP, as NO fails to increase cGMP production and cGMP analogs do not reproduce NO effect. NO does not modify the production of cAMP, the main second messenger that mediates gonadotropin action. When we studied NO effect over the steroidogenic pathway in MA-10 cells, we found that NO is inhibiting the conversion of cholesterol to pregnenolone. Taken together these results show an inhibitory effect of NO donors on Leydig cell steroidogenesis and suggest that NO can be directly inhibiting cholesterol side-chain cleavage enzyme (cytochrome P-450scc) as it does with other heme proteins, including different cytochromes P-450. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00257680_v57_n3_p337_DelPunta
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cholesterol cholesterol monooxygenase (side chain cleaving) chorionic gonadotropin cyclic gmp cyclic gmp derivative hemoprotein nitric oxide nitric oxide donor pregnenolone nitric oxide steroid animal cell animal tissue article inhibition kinetics leydig cell macrophage activation mouse nonhuman pathophysiology rat second messenger steroidogenesis testis cell animal biosynthesis disease model Leydig cell tumor male metabolism physiology Sprague Dawley rat testis tumor Animals Disease Models, Animal Leydig Cell Tumor Male Mice Nitric Oxide Rats Rats, Sprague-Dawley Steroids Testicular Neoplasms
spellingShingle	cholesterol cholesterol monooxygenase (side chain cleaving) chorionic gonadotropin cyclic gmp cyclic gmp derivative hemoprotein nitric oxide nitric oxide donor pregnenolone nitric oxide steroid animal cell animal tissue article inhibition kinetics leydig cell macrophage activation mouse nonhuman pathophysiology rat second messenger steroidogenesis testis cell animal biosynthesis disease model Leydig cell tumor male metabolism physiology Sprague Dawley rat testis tumor Animals Disease Models, Animal Leydig Cell Tumor Male Mice Nitric Oxide Rats Rats, Sprague-Dawley Steroids Testicular Neoplasms Del Punta, K. Sanchez-Ruiz, M.E. Pignataro, O.P. Regulation of testicular steroidogenesis by nitric oxide
topic_facet	cholesterol cholesterol monooxygenase (side chain cleaving) chorionic gonadotropin cyclic gmp cyclic gmp derivative hemoprotein nitric oxide nitric oxide donor pregnenolone nitric oxide steroid animal cell animal tissue article inhibition kinetics leydig cell macrophage activation mouse nonhuman pathophysiology rat second messenger steroidogenesis testis cell animal biosynthesis disease model Leydig cell tumor male metabolism physiology Sprague Dawley rat testis tumor Animals Disease Models, Animal Leydig Cell Tumor Male Mice Nitric Oxide Rats Rats, Sprague-Dawley Steroids Testicular Neoplasms
description	Testicular macrophages as well as endothelial cells, which are intimately associated with Leydig cells, constitute a potential source of paracrine nitric oxide (NO). In the present study, we investigated the effect of NO donors on MA-10 murine Leydig tumor cell line and rat Leydig cell steroidogenesis. We observed that NO donors, reversibly inhibit hGG-induced steroidogenesis in both types of cells. We also studied NO mechanism of action. Contrary to what is observed in many other systems, NO inhibitory effect on Leydig cell steroidogenesis is not mediated by cGMP, as NO fails to increase cGMP production and cGMP analogs do not reproduce NO effect. NO does not modify the production of cAMP, the main second messenger that mediates gonadotropin action. When we studied NO effect over the steroidogenic pathway in MA-10 cells, we found that NO is inhibiting the conversion of cholesterol to pregnenolone. Taken together these results show an inhibitory effect of NO donors on Leydig cell steroidogenesis and suggest that NO can be directly inhibiting cholesterol side-chain cleavage enzyme (cytochrome P-450scc) as it does with other heme proteins, including different cytochromes P-450.
format	JOUR
author	Del Punta, K. Sanchez-Ruiz, M.E. Pignataro, O.P.
author_facet	Del Punta, K. Sanchez-Ruiz, M.E. Pignataro, O.P.
author_sort	Del Punta, K.
title	Regulation of testicular steroidogenesis by nitric oxide
title_short	Regulation of testicular steroidogenesis by nitric oxide
title_full	Regulation of testicular steroidogenesis by nitric oxide
title_fullStr	Regulation of testicular steroidogenesis by nitric oxide
title_full_unstemmed	Regulation of testicular steroidogenesis by nitric oxide
title_sort	regulation of testicular steroidogenesis by nitric oxide
url	http://hdl.handle.net/20.500.12110/paper_00257680_v57_n3_p337_DelPunta
work_keys_str_mv	AT delpuntak regulationoftesticularsteroidogenesisbynitricoxide AT sanchezruizme regulationoftesticularsteroidogenesisbynitricoxide AT pignataroop regulationoftesticularsteroidogenesisbynitricoxide
_version_	1807317550752595968

Regulation of testicular steroidogenesis by nitric oxide

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