Regulation of follicular luteinization by an agonist of gonadotropin-releasing hormone (GnRH)
Long treatments with GnRH agonist are used in patients to suppress the endogenous secretion of gonadotropins, however; these analogs have a direct effect on the ovary. The aim of this work was to study the in vivo effect of the GnRH analogue, leuprolide acetate (LA) on ovarian steroidogenesis and ap...
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todo:paper_00257680_v57_n1_p83_Andreu2023-10-03T14:36:31Z Regulation of follicular luteinization by an agonist of gonadotropin-releasing hormone (GnRH) Andreu, C. Parborell, F. Vanzulli, S. Tesone, M. bucladesine chorionic gonadotropin cyclic amp gonadorelin agonist leuprorelin seric gonadotropin antineoplastic hormone agonists and antagonists animal experiment animal tissue apoptosis article controlled study drug effect female nonhuman ovary rat regulatory mechanism steroidogenesis animal corpus luteum follicular phase physiology Sprague Dawley rat Animals Antineoplastic Agents, Hormonal Corpus Luteum Female Follicular Phase Leuprolide Rats Rats, Sprague-Dawley Long treatments with GnRH agonist are used in patients to suppress the endogenous secretion of gonadotropins, however; these analogs have a direct effect on the ovary. The aim of this work was to study the in vivo effect of the GnRH analogue, leuprolide acetate (LA) on ovarian steroidogenesis and apoptosis mechanisms. LA (1 μg/rat/day) was injected to PMSG/hCG superovulated rats. Corpora lutea were isolated by microdissection and incubated (4/0.5 ml) during 3 h with LH (10 ng/ml) for dibutyryl cAMP (dcAMP 1 mM). Progesterone production was measured observing in LA treated rats a decrease in basal and LH stimulated values (Basal = Control C: 96.6 ± 9.6; LA: 22.9 ± 2.8; LH = C: 145.7 ± 4.9; LA: 23.6 ± 2.0 ng/ml, p < 0,001). In contrast, dcAMP stimulated significantly both groups (C: 153.9 ± 11.8; LA: 83.15 ± 8.2). cAMP production was lower in LA corpora lutea and LH was not able to stimulate them (Basal = C: 7.29 ± 1.6; LA: 1.17 ± 0.6; LH = C: 13.2 ± 0.4; LA: 2.5 ± 0.4 ng/ml, p < 0.01). Corpus luteum of both groups showed similar protein content. On the other hand, taking into account that in ovarian histological slides of LA treated rats we observed more atresic follicles and less corpora lutea, we determined the amount of apoptotic cells. The criterion used was the presence of apoptotic bodies and nuclear chromatin aggregated in dense masses beneath the nuclear envelope. An increase of apoptotic cells in the LA group ovaries was detected. This result was confirmed by immunohistochemical techniques (TUNEL). It was concluded that LA treatment produces in ovarian cells a failure in the LH receptor-adenylate cyclase system and an increase in cellular apoptosis. Fil:Andreu, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Parborell, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tesone, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00257680_v57_n1_p83_Andreu |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
bucladesine chorionic gonadotropin cyclic amp gonadorelin agonist leuprorelin seric gonadotropin antineoplastic hormone agonists and antagonists animal experiment animal tissue apoptosis article controlled study drug effect female nonhuman ovary rat regulatory mechanism steroidogenesis animal corpus luteum follicular phase physiology Sprague Dawley rat Animals Antineoplastic Agents, Hormonal Corpus Luteum Female Follicular Phase Leuprolide Rats Rats, Sprague-Dawley |
| spellingShingle |
bucladesine chorionic gonadotropin cyclic amp gonadorelin agonist leuprorelin seric gonadotropin antineoplastic hormone agonists and antagonists animal experiment animal tissue apoptosis article controlled study drug effect female nonhuman ovary rat regulatory mechanism steroidogenesis animal corpus luteum follicular phase physiology Sprague Dawley rat Animals Antineoplastic Agents, Hormonal Corpus Luteum Female Follicular Phase Leuprolide Rats Rats, Sprague-Dawley Andreu, C. Parborell, F. Vanzulli, S. Tesone, M. Regulation of follicular luteinization by an agonist of gonadotropin-releasing hormone (GnRH) |
| topic_facet |
bucladesine chorionic gonadotropin cyclic amp gonadorelin agonist leuprorelin seric gonadotropin antineoplastic hormone agonists and antagonists animal experiment animal tissue apoptosis article controlled study drug effect female nonhuman ovary rat regulatory mechanism steroidogenesis animal corpus luteum follicular phase physiology Sprague Dawley rat Animals Antineoplastic Agents, Hormonal Corpus Luteum Female Follicular Phase Leuprolide Rats Rats, Sprague-Dawley |
| description |
Long treatments with GnRH agonist are used in patients to suppress the endogenous secretion of gonadotropins, however; these analogs have a direct effect on the ovary. The aim of this work was to study the in vivo effect of the GnRH analogue, leuprolide acetate (LA) on ovarian steroidogenesis and apoptosis mechanisms. LA (1 μg/rat/day) was injected to PMSG/hCG superovulated rats. Corpora lutea were isolated by microdissection and incubated (4/0.5 ml) during 3 h with LH (10 ng/ml) for dibutyryl cAMP (dcAMP 1 mM). Progesterone production was measured observing in LA treated rats a decrease in basal and LH stimulated values (Basal = Control C: 96.6 ± 9.6; LA: 22.9 ± 2.8; LH = C: 145.7 ± 4.9; LA: 23.6 ± 2.0 ng/ml, p < 0,001). In contrast, dcAMP stimulated significantly both groups (C: 153.9 ± 11.8; LA: 83.15 ± 8.2). cAMP production was lower in LA corpora lutea and LH was not able to stimulate them (Basal = C: 7.29 ± 1.6; LA: 1.17 ± 0.6; LH = C: 13.2 ± 0.4; LA: 2.5 ± 0.4 ng/ml, p < 0.01). Corpus luteum of both groups showed similar protein content. On the other hand, taking into account that in ovarian histological slides of LA treated rats we observed more atresic follicles and less corpora lutea, we determined the amount of apoptotic cells. The criterion used was the presence of apoptotic bodies and nuclear chromatin aggregated in dense masses beneath the nuclear envelope. An increase of apoptotic cells in the LA group ovaries was detected. This result was confirmed by immunohistochemical techniques (TUNEL). It was concluded that LA treatment produces in ovarian cells a failure in the LH receptor-adenylate cyclase system and an increase in cellular apoptosis. |
| format |
JOUR |
| author |
Andreu, C. Parborell, F. Vanzulli, S. Tesone, M. |
| author_facet |
Andreu, C. Parborell, F. Vanzulli, S. Tesone, M. |
| author_sort |
Andreu, C. |
| title |
Regulation of follicular luteinization by an agonist of gonadotropin-releasing hormone (GnRH) |
| title_short |
Regulation of follicular luteinization by an agonist of gonadotropin-releasing hormone (GnRH) |
| title_full |
Regulation of follicular luteinization by an agonist of gonadotropin-releasing hormone (GnRH) |
| title_fullStr |
Regulation of follicular luteinization by an agonist of gonadotropin-releasing hormone (GnRH) |
| title_full_unstemmed |
Regulation of follicular luteinization by an agonist of gonadotropin-releasing hormone (GnRH) |
| title_sort |
regulation of follicular luteinization by an agonist of gonadotropin-releasing hormone (gnrh) |
| url |
http://hdl.handle.net/20.500.12110/paper_00257680_v57_n1_p83_Andreu |
| work_keys_str_mv |
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| _version_ |
1807322155878187008 |