A lineal equation for the classification of progressive and hyperactive spermatozoa
Capacitation of spermatozoa, a complex sequence of events that render them able to fertilize the egg, is generally associated with a switch from lineal, progressive movement to a vigorous, non-progressive pattern characterized by starlike tracks, a process known as hyperactivation. Development of a...
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todo:paper_00255564_v149_n1_p77_Vulcano2023-10-03T14:36:06Z A lineal equation for the classification of progressive and hyperactive spermatozoa Vulcano, G.J. Moses, D.F. Valcárcel, A. De Las Heras, M.A. article fertilization mathematical analysis mathematical model semen analysis spermatozoon capacitation spermatozoon maturation spermatozoon migration spermatozoon motility spermatozoon penetration Animals Kinetics Linear Models Male Mathematics Sheep Sperm Capacitation Sperm Motility Spermatozoa Capacitation of spermatozoa, a complex sequence of events that render them able to fertilize the egg, is generally associated with a switch from lineal, progressive movement to a vigorous, non-progressive pattern characterized by starlike tracks, a process known as hyperactivation. Development of a method for the analysis of progressive and hyperactive tracks is thus important for the assessment of capacitation in biochemical, physiological and clinical studies. In this study, we have applied a two- step heuristic model to deduce a lineal equation that discriminates hyperactive from progressive spermatozoa. The kinetic parameters (curvilinear velocity (VCL), linearity (LIN), amplitude of lateral head displacement (ALH), straightness (STR), wobble (WOB), mean 'dance' (DAN) and velocity of the average path (VAP)) of ram spermatozoa were evaluated with a computerized motility analyzer, and classified one by one as progressive or hyperactive by the appearance of their tracks. In a first step, a discriminating plane was defined by minimizing the number of misclassified spermatozoa ('conflicting points'); then, the plane was adjusted by an iterative process to minimize the distance from conflicting points to it. The resulting plane showed a discriminating capacity of over 95% for both classes, higher than that achieved by setting a threshold value for the parameters taken separately or in group. When included in a standard semen analysis, application of the equation allowed a rapid assessment of the percentage of hyperactive spermatozoa. The method described, developed in ram spermatozoa, can be applied to different species for a variety of purposes. Fil:Vulcano, G.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Moses, D.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Valcárcel, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:De Las Heras, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00255564_v149_n1_p77_Vulcano |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
article fertilization mathematical analysis mathematical model semen analysis spermatozoon capacitation spermatozoon maturation spermatozoon migration spermatozoon motility spermatozoon penetration Animals Kinetics Linear Models Male Mathematics Sheep Sperm Capacitation Sperm Motility Spermatozoa |
| spellingShingle |
article fertilization mathematical analysis mathematical model semen analysis spermatozoon capacitation spermatozoon maturation spermatozoon migration spermatozoon motility spermatozoon penetration Animals Kinetics Linear Models Male Mathematics Sheep Sperm Capacitation Sperm Motility Spermatozoa Vulcano, G.J. Moses, D.F. Valcárcel, A. De Las Heras, M.A. A lineal equation for the classification of progressive and hyperactive spermatozoa |
| topic_facet |
article fertilization mathematical analysis mathematical model semen analysis spermatozoon capacitation spermatozoon maturation spermatozoon migration spermatozoon motility spermatozoon penetration Animals Kinetics Linear Models Male Mathematics Sheep Sperm Capacitation Sperm Motility Spermatozoa |
| description |
Capacitation of spermatozoa, a complex sequence of events that render them able to fertilize the egg, is generally associated with a switch from lineal, progressive movement to a vigorous, non-progressive pattern characterized by starlike tracks, a process known as hyperactivation. Development of a method for the analysis of progressive and hyperactive tracks is thus important for the assessment of capacitation in biochemical, physiological and clinical studies. In this study, we have applied a two- step heuristic model to deduce a lineal equation that discriminates hyperactive from progressive spermatozoa. The kinetic parameters (curvilinear velocity (VCL), linearity (LIN), amplitude of lateral head displacement (ALH), straightness (STR), wobble (WOB), mean 'dance' (DAN) and velocity of the average path (VAP)) of ram spermatozoa were evaluated with a computerized motility analyzer, and classified one by one as progressive or hyperactive by the appearance of their tracks. In a first step, a discriminating plane was defined by minimizing the number of misclassified spermatozoa ('conflicting points'); then, the plane was adjusted by an iterative process to minimize the distance from conflicting points to it. The resulting plane showed a discriminating capacity of over 95% for both classes, higher than that achieved by setting a threshold value for the parameters taken separately or in group. When included in a standard semen analysis, application of the equation allowed a rapid assessment of the percentage of hyperactive spermatozoa. The method described, developed in ram spermatozoa, can be applied to different species for a variety of purposes. |
| format |
JOUR |
| author |
Vulcano, G.J. Moses, D.F. Valcárcel, A. De Las Heras, M.A. |
| author_facet |
Vulcano, G.J. Moses, D.F. Valcárcel, A. De Las Heras, M.A. |
| author_sort |
Vulcano, G.J. |
| title |
A lineal equation for the classification of progressive and hyperactive spermatozoa |
| title_short |
A lineal equation for the classification of progressive and hyperactive spermatozoa |
| title_full |
A lineal equation for the classification of progressive and hyperactive spermatozoa |
| title_fullStr |
A lineal equation for the classification of progressive and hyperactive spermatozoa |
| title_full_unstemmed |
A lineal equation for the classification of progressive and hyperactive spermatozoa |
| title_sort |
lineal equation for the classification of progressive and hyperactive spermatozoa |
| url |
http://hdl.handle.net/20.500.12110/paper_00255564_v149_n1_p77_Vulcano |
| work_keys_str_mv |
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