Culture studies on the mycobiont of Caloplaca erythrantha (Tuck.) Zahlbr. (Teloschistaceae): High production of major lichen secondary metabolites

A strain of the lichen mycobiont of Caloplaca erythrantha, isolated from ascospores, was cultured axenically on different solid media. Four of the media employed supported the development of colonies and production of the two major lichen secondary metabolites. These media were: BMYE (mannitol 2%, y...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Fazio, A.T., Adler, M.T., Bertoni, M.D., Maier, M.S.
Formato: JOUR
Materias:
7-chloroemodin
anthraquinones
axenic cultures
emodin
HPLC
spectroscopic methods
Caloplaca erythrantha
Fungi
Teloschistaceae
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00242829_v44_n4_p533_Fazio
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00242829_v44_n4_p533_Fazio
record_format dspace
spelling todo:paper_00242829_v44_n4_p533_Fazio2023-10-03T14:34:32Z Culture studies on the mycobiont of Caloplaca erythrantha (Tuck.) Zahlbr. (Teloschistaceae): High production of major lichen secondary metabolites Fazio, A.T. Adler, M.T. Bertoni, M.D. Maier, M.S. 7-chloroemodin anthraquinones axenic cultures emodin HPLC spectroscopic methods Caloplaca erythrantha Fungi Teloschistaceae A strain of the lichen mycobiont of Caloplaca erythrantha, isolated from ascospores, was cultured axenically on different solid media. Four of the media employed supported the development of colonies and production of the two major lichen secondary metabolites. These media were: BMYE (mannitol 2%, yeast extract 0.1%, in Bold's basal medium); MEYE (malt extract 2%, yeast extract 0.2%, in distilled water); Hamada's MY10 (malt extract 1%, yeast extract 0.4%, sucrose 10%, in distilled water); and the new BMRM (Bold mannitol rich medium, mannitol 5.3%, malt extract 1%, yeast extract 0.4% in Bold's mineral medium). Percentages refer to final medium volume. The fungal colonies developed well on the four media and produced emodin and 7-chloroemodin, the major secondary compounds of the lichen apothecia. Crystals deposited richly on the external surface of the hyphae, as observed with an optical microscope. The two anthraquinones were purified from the lichen thallus, apothecia and cultured mycelia, and identified by chromatographic (TLC, HPLC) and spectroscopic (NMR, MS) methods. The analysis of lichen apothecia revealed the presence of emodin (0.90% w/w) and 7-chloroemodin (0.56% w/w), whereas colonies cultured for five months generally produced higher percentages than the lichen: 1.72% emodin and 0.30% 7-chloroemodin on BMYE; 0.21% and 0.95% on MEYE; 7.82% and 7.48% on MY10; and 11.70% and 10.80% on BMRM. These results show that the production of both anthraquinones was promoted significantly in mycobiont cultures with high concentrations of the carbon sources sucrose or mannitol, with a higher effect being observed with the latter. © 2012 British Lichen Society. Fil:Fazio, A.T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Adler, M.T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bertoni, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maier, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00242829_v44_n4_p533_Fazio
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 7-chloroemodin
anthraquinones
axenic cultures
emodin
HPLC
spectroscopic methods
Caloplaca erythrantha
Fungi
Teloschistaceae
spellingShingle 7-chloroemodin
anthraquinones
axenic cultures
emodin
HPLC
spectroscopic methods
Caloplaca erythrantha
Fungi
Teloschistaceae
Fazio, A.T.
Adler, M.T.
Bertoni, M.D.
Maier, M.S.
Culture studies on the mycobiont of Caloplaca erythrantha (Tuck.) Zahlbr. (Teloschistaceae): High production of major lichen secondary metabolites
topic_facet 7-chloroemodin
anthraquinones
axenic cultures
emodin
HPLC
spectroscopic methods
Caloplaca erythrantha
Fungi
Teloschistaceae
description A strain of the lichen mycobiont of Caloplaca erythrantha, isolated from ascospores, was cultured axenically on different solid media. Four of the media employed supported the development of colonies and production of the two major lichen secondary metabolites. These media were: BMYE (mannitol 2%, yeast extract 0.1%, in Bold's basal medium); MEYE (malt extract 2%, yeast extract 0.2%, in distilled water); Hamada's MY10 (malt extract 1%, yeast extract 0.4%, sucrose 10%, in distilled water); and the new BMRM (Bold mannitol rich medium, mannitol 5.3%, malt extract 1%, yeast extract 0.4% in Bold's mineral medium). Percentages refer to final medium volume. The fungal colonies developed well on the four media and produced emodin and 7-chloroemodin, the major secondary compounds of the lichen apothecia. Crystals deposited richly on the external surface of the hyphae, as observed with an optical microscope. The two anthraquinones were purified from the lichen thallus, apothecia and cultured mycelia, and identified by chromatographic (TLC, HPLC) and spectroscopic (NMR, MS) methods. The analysis of lichen apothecia revealed the presence of emodin (0.90% w/w) and 7-chloroemodin (0.56% w/w), whereas colonies cultured for five months generally produced higher percentages than the lichen: 1.72% emodin and 0.30% 7-chloroemodin on BMYE; 0.21% and 0.95% on MEYE; 7.82% and 7.48% on MY10; and 11.70% and 10.80% on BMRM. These results show that the production of both anthraquinones was promoted significantly in mycobiont cultures with high concentrations of the carbon sources sucrose or mannitol, with a higher effect being observed with the latter. © 2012 British Lichen Society.
format JOUR
author Fazio, A.T.
Adler, M.T.
Bertoni, M.D.
Maier, M.S.
author_facet Fazio, A.T.
Adler, M.T.
Bertoni, M.D.
Maier, M.S.
author_sort Fazio, A.T.
title Culture studies on the mycobiont of Caloplaca erythrantha (Tuck.) Zahlbr. (Teloschistaceae): High production of major lichen secondary metabolites
title_short Culture studies on the mycobiont of Caloplaca erythrantha (Tuck.) Zahlbr. (Teloschistaceae): High production of major lichen secondary metabolites
title_full Culture studies on the mycobiont of Caloplaca erythrantha (Tuck.) Zahlbr. (Teloschistaceae): High production of major lichen secondary metabolites
title_fullStr Culture studies on the mycobiont of Caloplaca erythrantha (Tuck.) Zahlbr. (Teloschistaceae): High production of major lichen secondary metabolites
title_full_unstemmed Culture studies on the mycobiont of Caloplaca erythrantha (Tuck.) Zahlbr. (Teloschistaceae): High production of major lichen secondary metabolites
title_sort culture studies on the mycobiont of caloplaca erythrantha (tuck.) zahlbr. (teloschistaceae): high production of major lichen secondary metabolites
url http://hdl.handle.net/20.500.12110/paper_00242829_v44_n4_p533_Fazio
work_keys_str_mv AT fazioat culturestudiesonthemycobiontofcaloplacaerythranthatuckzahlbrteloschistaceaehighproductionofmajorlichensecondarymetabolites
AT adlermt culturestudiesonthemycobiontofcaloplacaerythranthatuckzahlbrteloschistaceaehighproductionofmajorlichensecondarymetabolites
AT bertonimd culturestudiesonthemycobiontofcaloplacaerythranthatuckzahlbrteloschistaceaehighproductionofmajorlichensecondarymetabolites
AT maierms culturestudiesonthemycobiontofcaloplacaerythranthatuckzahlbrteloschistaceaehighproductionofmajorlichensecondarymetabolites
_version_ 1807316409518129152

Ejemplares similares