Improved bile-acid thin-layer chromatography to identify feces of neotropical carnivores

A modified thin-layer chromatography was used to identify carnivore feces based on their bile-acid patterns. Our method is safer and makes the identification of bile acids more objective. We applied the technique to feces of 2 sympatric canids in the Neotropics, the culpeo (Pseudalopex culpaeus) and...

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Autores principales: Capurro, A.F., Novaro, A.J., Travaini, A., Romero, M.S.
Formato: JOUR
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Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0022541X_v61_n4_p1424_Capurro
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spelling todo:paper_0022541X_v61_n4_p1424_Capurro2023-10-03T14:33:42Z Improved bile-acid thin-layer chromatography to identify feces of neotropical carnivores Capurro, A.F. Novaro, A.J. Travaini, A. Romero, M.S. Canids Culpeo Fecal bile acids Foxes Neotropical carnivores Pseudalopex culpaeus Pseudalopex griseus South America South American gray fox Steroid Thin-layer chromatography bile acid bile acod faeces identification thin-layer chromatography Canidae Pseudalopex culpaeus Pseudalopex culpaeus Pseudalopex griseus Pseudalopex griseus Urocyon cinereoargenteus Vertebrata A modified thin-layer chromatography was used to identify carnivore feces based on their bile-acid patterns. Our method is safer and makes the identification of bile acids more objective. We applied the technique to feces of 2 sympatric canids in the Neotropics, the culpeo (Pseudalopex culpaeus) and South American gray fox (P. griseus), and describe bile-acid profiles of 7 other species from the region. Presence of chenodeoxycholic acid and hyodeoxycholic acids in feces of culpeo foxes allowed us to distinguish them from gray-fox feces with 85% accuracy. The method is most appropriate for carnivore species that consume small amounts of fiber and occur in dry habitats. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0022541X_v61_n4_p1424_Capurro
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Canids
Culpeo
Fecal bile acids
Foxes
Neotropical carnivores
Pseudalopex culpaeus
Pseudalopex griseus
South America
South American gray fox
Steroid
Thin-layer chromatography
bile acid
bile acod
faeces
identification
thin-layer chromatography
Canidae
Pseudalopex culpaeus
Pseudalopex culpaeus
Pseudalopex griseus
Pseudalopex griseus
Urocyon cinereoargenteus
Vertebrata
spellingShingle Canids
Culpeo
Fecal bile acids
Foxes
Neotropical carnivores
Pseudalopex culpaeus
Pseudalopex griseus
South America
South American gray fox
Steroid
Thin-layer chromatography
bile acid
bile acod
faeces
identification
thin-layer chromatography
Canidae
Pseudalopex culpaeus
Pseudalopex culpaeus
Pseudalopex griseus
Pseudalopex griseus
Urocyon cinereoargenteus
Vertebrata
Capurro, A.F.
Novaro, A.J.
Travaini, A.
Romero, M.S.
Improved bile-acid thin-layer chromatography to identify feces of neotropical carnivores
topic_facet Canids
Culpeo
Fecal bile acids
Foxes
Neotropical carnivores
Pseudalopex culpaeus
Pseudalopex griseus
South America
South American gray fox
Steroid
Thin-layer chromatography
bile acid
bile acod
faeces
identification
thin-layer chromatography
Canidae
Pseudalopex culpaeus
Pseudalopex culpaeus
Pseudalopex griseus
Pseudalopex griseus
Urocyon cinereoargenteus
Vertebrata
description A modified thin-layer chromatography was used to identify carnivore feces based on their bile-acid patterns. Our method is safer and makes the identification of bile acids more objective. We applied the technique to feces of 2 sympatric canids in the Neotropics, the culpeo (Pseudalopex culpaeus) and South American gray fox (P. griseus), and describe bile-acid profiles of 7 other species from the region. Presence of chenodeoxycholic acid and hyodeoxycholic acids in feces of culpeo foxes allowed us to distinguish them from gray-fox feces with 85% accuracy. The method is most appropriate for carnivore species that consume small amounts of fiber and occur in dry habitats.
format JOUR
author Capurro, A.F.
Novaro, A.J.
Travaini, A.
Romero, M.S.
author_facet Capurro, A.F.
Novaro, A.J.
Travaini, A.
Romero, M.S.
author_sort Capurro, A.F.
title Improved bile-acid thin-layer chromatography to identify feces of neotropical carnivores
title_short Improved bile-acid thin-layer chromatography to identify feces of neotropical carnivores
title_full Improved bile-acid thin-layer chromatography to identify feces of neotropical carnivores
title_fullStr Improved bile-acid thin-layer chromatography to identify feces of neotropical carnivores
title_full_unstemmed Improved bile-acid thin-layer chromatography to identify feces of neotropical carnivores
title_sort improved bile-acid thin-layer chromatography to identify feces of neotropical carnivores
url http://hdl.handle.net/20.500.12110/paper_0022541X_v61_n4_p1424_Capurro
work_keys_str_mv AT capurroaf improvedbileacidthinlayerchromatographytoidentifyfecesofneotropicalcarnivores
AT novaroaj improvedbileacidthinlayerchromatographytoidentifyfecesofneotropicalcarnivores
AT travainia improvedbileacidthinlayerchromatographytoidentifyfecesofneotropicalcarnivores
AT romeroms improvedbileacidthinlayerchromatographytoidentifyfecesofneotropicalcarnivores
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