Does the coupling between conformational fluctuation and enzyme catalysis involve a true phase transfer catalysis?
A theoretical model of the coupling between conformational fluctuation of the protein matrix and enzyme catalysis is here proposed. The model rests on the following assumptions: (1) The active site of the enzyme molecule can exist in at least two thermodynamically stable states: open and closed; (2)...
Guardado en:
| Autor principal: | |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00225193_v99_n1_p21_Olavarria |
| Aporte de: |
| id |
todo:paper_00225193_v99_n1_p21_Olavarria |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_00225193_v99_n1_p21_Olavarria2023-10-03T14:33:39Z Does the coupling between conformational fluctuation and enzyme catalysis involve a true phase transfer catalysis? Olavarría, J. enzyme allosterism article binding site biological model catalysis kinetics metabolism protein conformation Allosteric Regulation Binding Sites Catalysis Enzymes Kinetics Models, Biological Protein Conformation A theoretical model of the coupling between conformational fluctuation of the protein matrix and enzyme catalysis is here proposed. The model rests on the following assumptions: (1) The active site of the enzyme molecule can exist in at least two thermodynamically stable states: open and closed; (2) only the open conformation exchange substrates with the medium; (3) the enzyme in the closed conformation acquired over its substrate the maximal catalytic activity, (4) the fluctuation of the enzyme molecule between both conformations is an absolute requirement for the completion of a catalytical cycle. The parallelism of this cyclic catalytic process with phase transfer catalysis is discussed. Positive and negative cooperativity due to the coupling of conformational fluctuation in oligomers is predicted by the model. The same is true for half of the sites reactivity. Other kinetic features of the model are discussed. © 1982. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00225193_v99_n1_p21_Olavarria |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
enzyme allosterism article binding site biological model catalysis kinetics metabolism protein conformation Allosteric Regulation Binding Sites Catalysis Enzymes Kinetics Models, Biological Protein Conformation |
| spellingShingle |
enzyme allosterism article binding site biological model catalysis kinetics metabolism protein conformation Allosteric Regulation Binding Sites Catalysis Enzymes Kinetics Models, Biological Protein Conformation Olavarría, J. Does the coupling between conformational fluctuation and enzyme catalysis involve a true phase transfer catalysis? |
| topic_facet |
enzyme allosterism article binding site biological model catalysis kinetics metabolism protein conformation Allosteric Regulation Binding Sites Catalysis Enzymes Kinetics Models, Biological Protein Conformation |
| description |
A theoretical model of the coupling between conformational fluctuation of the protein matrix and enzyme catalysis is here proposed. The model rests on the following assumptions: (1) The active site of the enzyme molecule can exist in at least two thermodynamically stable states: open and closed; (2) only the open conformation exchange substrates with the medium; (3) the enzyme in the closed conformation acquired over its substrate the maximal catalytic activity, (4) the fluctuation of the enzyme molecule between both conformations is an absolute requirement for the completion of a catalytical cycle. The parallelism of this cyclic catalytic process with phase transfer catalysis is discussed. Positive and negative cooperativity due to the coupling of conformational fluctuation in oligomers is predicted by the model. The same is true for half of the sites reactivity. Other kinetic features of the model are discussed. © 1982. |
| format |
JOUR |
| author |
Olavarría, J. |
| author_facet |
Olavarría, J. |
| author_sort |
Olavarría, J. |
| title |
Does the coupling between conformational fluctuation and enzyme catalysis involve a true phase transfer catalysis? |
| title_short |
Does the coupling between conformational fluctuation and enzyme catalysis involve a true phase transfer catalysis? |
| title_full |
Does the coupling between conformational fluctuation and enzyme catalysis involve a true phase transfer catalysis? |
| title_fullStr |
Does the coupling between conformational fluctuation and enzyme catalysis involve a true phase transfer catalysis? |
| title_full_unstemmed |
Does the coupling between conformational fluctuation and enzyme catalysis involve a true phase transfer catalysis? |
| title_sort |
does the coupling between conformational fluctuation and enzyme catalysis involve a true phase transfer catalysis? |
| url |
http://hdl.handle.net/20.500.12110/paper_00225193_v99_n1_p21_Olavarria |
| work_keys_str_mv |
AT olavarriaj doesthecouplingbetweenconformationalfluctuationandenzymecatalysisinvolveatruephasetransfercatalysis |
| _version_ |
1782027029251620864 |