Micellar electrokinetic capillary chromatography of methylxanthines- containing beverages: Discussion of the molecular species involved

Micellar electrokinetic capillary chromatography (MECC) experimental conditions were applied to 12 samples of methylxanthine-containing infusions of different commercial brands of yerba mate, coffee, tea and cocoa as well as two cola drinks. The best resolution in this mode of automated high-perform...

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Autores principales:	Pomilio, A.B., Trajtemberg, S.P., Vitale, A.A.
Formato:	JOUR
Materias:	Automated high-performance capillary electrophoresis (HPCE) Beverages Caffeine Keto-enol tautomerism Micellar electrokinetic capillary chromatography (MECC) Theobromine Theophylline Cola Croton ovalifolius Ilex paraguariensis Theobroma cacao
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00225142_v85_n4_p622_Pomilio
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00225142_v85_n4_p622_Pomilio
record_format	dspace
spelling	todo:paper_00225142_v85_n4_p622_Pomilio2023-10-03T14:33:32Z Micellar electrokinetic capillary chromatography of methylxanthines- containing beverages: Discussion of the molecular species involved Pomilio, A.B. Trajtemberg, S.P. Vitale, A.A. Automated high-performance capillary electrophoresis (HPCE) Beverages Caffeine Keto-enol tautomerism Micellar electrokinetic capillary chromatography (MECC) Theobromine Theophylline Cola Croton ovalifolius Ilex paraguariensis Theobroma cacao Micellar electrokinetic capillary chromatography (MECC) experimental conditions were applied to 12 samples of methylxanthine-containing infusions of different commercial brands of yerba mate, coffee, tea and cocoa as well as two cola drinks. The best resolution in this mode of automated high-performance capillary electrophoresis (HPCE) was achieved here when using 15 kV voltage in an uncoated fused-silica capillary of 45 cm length (40 cm effective length), 50 mM sodium dodecylsulfate, 90 mM pH 8.5 borate buffer and UV detection. Theobromine, caffeine and theophylline were separated, and the peak splitting due to tautomeric species was observed. Experimental conditions were controlled, keeping constant the size of the elution window in each analysis. The limit of detection was less than 1 mgl -1, the limit of quantitation was 2.5 mgl -1 and the work range was 2.5-300 mgl -1. This HPCE-MECC system has proved suitable for the analysis/quality control of xanthines in beverages for consumption. Roles of various parameters as well as distinctly charged species of each xanthine and the origin of peak splitting in this MECC system are discussed. © 2004 Society of Chemical Industry. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00225142_v85_n4_p622_Pomilio
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Automated high-performance capillary electrophoresis (HPCE) Beverages Caffeine Keto-enol tautomerism Micellar electrokinetic capillary chromatography (MECC) Theobromine Theophylline Cola Croton ovalifolius Ilex paraguariensis Theobroma cacao
spellingShingle	Automated high-performance capillary electrophoresis (HPCE) Beverages Caffeine Keto-enol tautomerism Micellar electrokinetic capillary chromatography (MECC) Theobromine Theophylline Cola Croton ovalifolius Ilex paraguariensis Theobroma cacao Pomilio, A.B. Trajtemberg, S.P. Vitale, A.A. Micellar electrokinetic capillary chromatography of methylxanthines- containing beverages: Discussion of the molecular species involved
topic_facet	Automated high-performance capillary electrophoresis (HPCE) Beverages Caffeine Keto-enol tautomerism Micellar electrokinetic capillary chromatography (MECC) Theobromine Theophylline Cola Croton ovalifolius Ilex paraguariensis Theobroma cacao
description	Micellar electrokinetic capillary chromatography (MECC) experimental conditions were applied to 12 samples of methylxanthine-containing infusions of different commercial brands of yerba mate, coffee, tea and cocoa as well as two cola drinks. The best resolution in this mode of automated high-performance capillary electrophoresis (HPCE) was achieved here when using 15 kV voltage in an uncoated fused-silica capillary of 45 cm length (40 cm effective length), 50 mM sodium dodecylsulfate, 90 mM pH 8.5 borate buffer and UV detection. Theobromine, caffeine and theophylline were separated, and the peak splitting due to tautomeric species was observed. Experimental conditions were controlled, keeping constant the size of the elution window in each analysis. The limit of detection was less than 1 mgl -1, the limit of quantitation was 2.5 mgl -1 and the work range was 2.5-300 mgl -1. This HPCE-MECC system has proved suitable for the analysis/quality control of xanthines in beverages for consumption. Roles of various parameters as well as distinctly charged species of each xanthine and the origin of peak splitting in this MECC system are discussed. © 2004 Society of Chemical Industry.
format	JOUR
author	Pomilio, A.B. Trajtemberg, S.P. Vitale, A.A.
author_facet	Pomilio, A.B. Trajtemberg, S.P. Vitale, A.A.
author_sort	Pomilio, A.B.
title	Micellar electrokinetic capillary chromatography of methylxanthines- containing beverages: Discussion of the molecular species involved
title_short	Micellar electrokinetic capillary chromatography of methylxanthines- containing beverages: Discussion of the molecular species involved
title_full	Micellar electrokinetic capillary chromatography of methylxanthines- containing beverages: Discussion of the molecular species involved
title_fullStr	Micellar electrokinetic capillary chromatography of methylxanthines- containing beverages: Discussion of the molecular species involved
title_full_unstemmed	Micellar electrokinetic capillary chromatography of methylxanthines- containing beverages: Discussion of the molecular species involved
title_sort	micellar electrokinetic capillary chromatography of methylxanthines- containing beverages: discussion of the molecular species involved
url	http://hdl.handle.net/20.500.12110/paper_00225142_v85_n4_p622_Pomilio
work_keys_str_mv	AT pomilioab micellarelectrokineticcapillarychromatographyofmethylxanthinescontainingbeveragesdiscussionofthemolecularspeciesinvolved AT trajtembergsp micellarelectrokineticcapillarychromatographyofmethylxanthinescontainingbeveragesdiscussionofthemolecularspeciesinvolved AT vitaleaa micellarelectrokineticcapillarychromatographyofmethylxanthinescontainingbeveragesdiscussionofthemolecularspeciesinvolved
_version_	1782027708881960960

Micellar electrokinetic capillary chromatography of methylxanthines- containing beverages: Discussion of the molecular species involved

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