Reduction in [3H]-corticosterone binding to cytoplasmic receptors in the brain of diabetic rats
The binding of [1, 2, 6, 7-3H]-corticosterone was studied in brain cytosol from normal and streptozotocin-diabetic male rats. The experiments were performed under conditions of incubation time (4h), temperature (0-4°C), time after adrenalectomy (6 days) and corticosterone concentrations (1.2 × 10-8...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00224731_v9_n7_p609_Fridman |
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todo:paper_00224731_v9_n7_p609_Fridman2023-10-03T14:33:14Z Reduction in [3H]-corticosterone binding to cytoplasmic receptors in the brain of diabetic rats Fridman, O. Foglia, V.G. De Nlcola, A.F. aldosterone corticosterone glucocorticoid receptor steroid receptor drug receptor hormone receptor radioisotope streptozocin adrenalectomy animal article binding competition brain cytosol experimental diabetes mellitus kinetics male metabolism rat temperature adrenalectomy aldosterone h 3 animal experiment brain central nervous system corticosterone h 3 cytoplasm cytosol diabetes mellitus endocrine system rat Adrenalectomy Aldosterone Animal Binding, Competitive Brain Corticosterone Cytosol Diabetes Mellitus, Experimental Kinetics Male Rats Receptors, Glucocorticoid Receptors, Steroid Temperature The binding of [1, 2, 6, 7-3H]-corticosterone was studied in brain cytosol from normal and streptozotocin-diabetic male rats. The experiments were performed under conditions of incubation time (4h), temperature (0-4°C), time after adrenalectomy (6 days) and corticosterone concentrations (1.2 × 10-8 and 1.15 × 10-9M) previously established for determining binding activity in the brain of normal rats. The binding of [3H]-corticosterone was found invariably lower in cytosol of the brain from diabetic rats, studied under three different conditions: in non-adrenalectomized animals, in adrenalectomized using a non-saturating corticosterone concentration, and in adrenalectomized plus a saturating steroid concentration. These results support previous contentions that the diminished sensitivity to the negative feedback for steroids which is present in diabetics, may be related to a reduction in binding capacity for corticoids in the central nervous system. © 1978. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00224731_v9_n7_p609_Fridman |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
aldosterone corticosterone glucocorticoid receptor steroid receptor drug receptor hormone receptor radioisotope streptozocin adrenalectomy animal article binding competition brain cytosol experimental diabetes mellitus kinetics male metabolism rat temperature adrenalectomy aldosterone h 3 animal experiment brain central nervous system corticosterone h 3 cytoplasm cytosol diabetes mellitus endocrine system rat Adrenalectomy Aldosterone Animal Binding, Competitive Brain Corticosterone Cytosol Diabetes Mellitus, Experimental Kinetics Male Rats Receptors, Glucocorticoid Receptors, Steroid Temperature |
| spellingShingle |
aldosterone corticosterone glucocorticoid receptor steroid receptor drug receptor hormone receptor radioisotope streptozocin adrenalectomy animal article binding competition brain cytosol experimental diabetes mellitus kinetics male metabolism rat temperature adrenalectomy aldosterone h 3 animal experiment brain central nervous system corticosterone h 3 cytoplasm cytosol diabetes mellitus endocrine system rat Adrenalectomy Aldosterone Animal Binding, Competitive Brain Corticosterone Cytosol Diabetes Mellitus, Experimental Kinetics Male Rats Receptors, Glucocorticoid Receptors, Steroid Temperature Fridman, O. Foglia, V.G. De Nlcola, A.F. Reduction in [3H]-corticosterone binding to cytoplasmic receptors in the brain of diabetic rats |
| topic_facet |
aldosterone corticosterone glucocorticoid receptor steroid receptor drug receptor hormone receptor radioisotope streptozocin adrenalectomy animal article binding competition brain cytosol experimental diabetes mellitus kinetics male metabolism rat temperature adrenalectomy aldosterone h 3 animal experiment brain central nervous system corticosterone h 3 cytoplasm cytosol diabetes mellitus endocrine system rat Adrenalectomy Aldosterone Animal Binding, Competitive Brain Corticosterone Cytosol Diabetes Mellitus, Experimental Kinetics Male Rats Receptors, Glucocorticoid Receptors, Steroid Temperature |
| description |
The binding of [1, 2, 6, 7-3H]-corticosterone was studied in brain cytosol from normal and streptozotocin-diabetic male rats. The experiments were performed under conditions of incubation time (4h), temperature (0-4°C), time after adrenalectomy (6 days) and corticosterone concentrations (1.2 × 10-8 and 1.15 × 10-9M) previously established for determining binding activity in the brain of normal rats. The binding of [3H]-corticosterone was found invariably lower in cytosol of the brain from diabetic rats, studied under three different conditions: in non-adrenalectomized animals, in adrenalectomized using a non-saturating corticosterone concentration, and in adrenalectomized plus a saturating steroid concentration. These results support previous contentions that the diminished sensitivity to the negative feedback for steroids which is present in diabetics, may be related to a reduction in binding capacity for corticoids in the central nervous system. © 1978. |
| format |
JOUR |
| author |
Fridman, O. Foglia, V.G. De Nlcola, A.F. |
| author_facet |
Fridman, O. Foglia, V.G. De Nlcola, A.F. |
| author_sort |
Fridman, O. |
| title |
Reduction in [3H]-corticosterone binding to cytoplasmic receptors in the brain of diabetic rats |
| title_short |
Reduction in [3H]-corticosterone binding to cytoplasmic receptors in the brain of diabetic rats |
| title_full |
Reduction in [3H]-corticosterone binding to cytoplasmic receptors in the brain of diabetic rats |
| title_fullStr |
Reduction in [3H]-corticosterone binding to cytoplasmic receptors in the brain of diabetic rats |
| title_full_unstemmed |
Reduction in [3H]-corticosterone binding to cytoplasmic receptors in the brain of diabetic rats |
| title_sort |
reduction in [3h]-corticosterone binding to cytoplasmic receptors in the brain of diabetic rats |
| url |
http://hdl.handle.net/20.500.12110/paper_00224731_v9_n7_p609_Fridman |
| work_keys_str_mv |
AT fridmano reductionin3hcorticosteronebindingtocytoplasmicreceptorsinthebrainofdiabeticrats AT fogliavg reductionin3hcorticosteronebindingtocytoplasmicreceptorsinthebrainofdiabeticrats AT denlcolaaf reductionin3hcorticosteronebindingtocytoplasmicreceptorsinthebrainofdiabeticrats |
| _version_ |
1807317189040013312 |