Versatile steroid molecules at the end of the aldosterone pathway

18-hydroxycorticosterone converts spontaneously and reversibly to a variety of less polar forms and derivatives, some of which are precursors to aldosterone. In particular, 21-hydroxy-11β,18-oxido-4-pregnene-3,20-dione (18-DAL) is hydroxylated to aldosterone with high yields in the presence of malat...

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Autores principales: Lantos, C.P., Damasco, M.C., Aragonés, A., Ceballos, N.R., Burton, G., Cozza, E.N.
Formato: JOUR
Materias:
aldosterone
hormone precursor
reduced nicotinamide adenine dinucleotide phosphate
cattle
metabolism
mitochondrion
nonhuman
short survey
18-Hydroxycorticosterone
Acid-Base Equilibrium
Adrenal Cortex
Aldosterone
Animal
Chemistry
Homeostasis
Hydrogen-Ion Concentration
Protein Precursors
Steroids
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00224731_v27_n4-6_p791_Lantos
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00224731_v27_n4-6_p791_Lantos
record_format dspace
spelling todo:paper_00224731_v27_n4-6_p791_Lantos2023-10-03T14:33:06Z Versatile steroid molecules at the end of the aldosterone pathway Lantos, C.P. Damasco, M.C. Aragonés, A. Ceballos, N.R. Burton, G. Cozza, E.N. aldosterone hormone precursor reduced nicotinamide adenine dinucleotide phosphate cattle metabolism mitochondrion nonhuman short survey 18-Hydroxycorticosterone Acid-Base Equilibrium Adrenal Cortex Aldosterone Animal Chemistry Homeostasis Hydrogen-Ion Concentration Protein Precursors Steroids 18-hydroxycorticosterone converts spontaneously and reversibly to a variety of less polar forms and derivatives, some of which are precursors to aldosterone. In particular, 21-hydroxy-11β,18-oxido-4-pregnene-3,20-dione (18-DAL) is hydroxylated to aldosterone with high yields in the presence of malate and NADP+ at pH 4.8.18-DAL also behaves as a metabolic intermediate between 18-OH-B and aldosterone accorcling to time-course and trapping experiments. Consequently, the final steps of the aldosterone pathway at pH 4.8 could be identified as 18-OH-B,18-DAL and aldosterone, in this sequence. The submitochondrial distribution of aldosterone biosynthesis is compatible with this postulate. The work also shows that some forms of 18-OH-B are promoters of hydrogen transport in renal tubuli and that this regulation may be independent of sodium reabsorption. These results suggest a regulatory model, new in steroid biology, accorcling to which steroid molecules bearing an oxidized angular C18-methyl may undergo structural changes between precursor ("P") and hormonal ("H") forms in response to homeostatic requirements. © 1987. Fil:Aragonés, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ceballos, N.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Burton, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cozza, E.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00224731_v27_n4-6_p791_Lantos
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic aldosterone
hormone precursor
reduced nicotinamide adenine dinucleotide phosphate
cattle
metabolism
mitochondrion
nonhuman
short survey
18-Hydroxycorticosterone
Acid-Base Equilibrium
Adrenal Cortex
Aldosterone
Animal
Chemistry
Homeostasis
Hydrogen-Ion Concentration
Protein Precursors
Steroids
spellingShingle aldosterone
hormone precursor
reduced nicotinamide adenine dinucleotide phosphate
cattle
metabolism
mitochondrion
nonhuman
short survey
18-Hydroxycorticosterone
Acid-Base Equilibrium
Adrenal Cortex
Aldosterone
Animal
Chemistry
Homeostasis
Hydrogen-Ion Concentration
Protein Precursors
Steroids
Lantos, C.P.
Damasco, M.C.
Aragonés, A.
Ceballos, N.R.
Burton, G.
Cozza, E.N.
Versatile steroid molecules at the end of the aldosterone pathway
topic_facet aldosterone
hormone precursor
reduced nicotinamide adenine dinucleotide phosphate
cattle
metabolism
mitochondrion
nonhuman
short survey
18-Hydroxycorticosterone
Acid-Base Equilibrium
Adrenal Cortex
Aldosterone
Animal
Chemistry
Homeostasis
Hydrogen-Ion Concentration
Protein Precursors
Steroids
description 18-hydroxycorticosterone converts spontaneously and reversibly to a variety of less polar forms and derivatives, some of which are precursors to aldosterone. In particular, 21-hydroxy-11β,18-oxido-4-pregnene-3,20-dione (18-DAL) is hydroxylated to aldosterone with high yields in the presence of malate and NADP+ at pH 4.8.18-DAL also behaves as a metabolic intermediate between 18-OH-B and aldosterone accorcling to time-course and trapping experiments. Consequently, the final steps of the aldosterone pathway at pH 4.8 could be identified as 18-OH-B,18-DAL and aldosterone, in this sequence. The submitochondrial distribution of aldosterone biosynthesis is compatible with this postulate. The work also shows that some forms of 18-OH-B are promoters of hydrogen transport in renal tubuli and that this regulation may be independent of sodium reabsorption. These results suggest a regulatory model, new in steroid biology, accorcling to which steroid molecules bearing an oxidized angular C18-methyl may undergo structural changes between precursor ("P") and hormonal ("H") forms in response to homeostatic requirements. © 1987.
format JOUR
author Lantos, C.P.
Damasco, M.C.
Aragonés, A.
Ceballos, N.R.
Burton, G.
Cozza, E.N.
author_facet Lantos, C.P.
Damasco, M.C.
Aragonés, A.
Ceballos, N.R.
Burton, G.
Cozza, E.N.
author_sort Lantos, C.P.
title Versatile steroid molecules at the end of the aldosterone pathway
title_short Versatile steroid molecules at the end of the aldosterone pathway
title_full Versatile steroid molecules at the end of the aldosterone pathway
title_fullStr Versatile steroid molecules at the end of the aldosterone pathway
title_full_unstemmed Versatile steroid molecules at the end of the aldosterone pathway
title_sort versatile steroid molecules at the end of the aldosterone pathway
url http://hdl.handle.net/20.500.12110/paper_00224731_v27_n4-6_p791_Lantos
work_keys_str_mv AT lantoscp versatilesteroidmoleculesattheendofthealdosteronepathway
AT damascomc versatilesteroidmoleculesattheendofthealdosteronepathway
AT aragonesa versatilesteroidmoleculesattheendofthealdosteronepathway
AT ceballosnr versatilesteroidmoleculesattheendofthealdosteronepathway
AT burtong versatilesteroidmoleculesattheendofthealdosteronepathway
AT cozzaen versatilesteroidmoleculesattheendofthealdosteronepathway
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