Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals

Samples containing as precursors either 18-hydroxycorticosterone (18-OH-B) in its M form, or this converted to less polar forms at pH 2 (ACM), or M or ACM enclosed in liposomes from adrenal lipids were incubated at pH 7.4, 4.8 or 3.3 in the presence or absence of quartered rat adrenals for 1 and 2 h...

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Autores principales: Cozza, E.N., Burton, G., Ceballos, N.R., Lantos, C.P., Harnik, M., Scott, A.I.
Formato: JOUR
Materias:
18 deoxyaldosterone
18 hydroxycorticosterone
aldosterone
radioisotope
unclassified drug
adrenal gland
animal cell
article
endocrine system
nonhuman
priority journal
rat
18-Hydroxycorticosterone
Adrenal Glands
Aldosterone
Animals
Corticosterone
Hydrogen-Ion Concentration
Liposomes
Magnetic Resonance Spectroscopy
Male
Rats
Animalia
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00224731_v22_n5_p665_Cozza
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00224731_v22_n5_p665_Cozza
record_format dspace
spelling todo:paper_00224731_v22_n5_p665_Cozza2023-10-03T14:33:04Z Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals Cozza, E.N. Burton, G. Ceballos, N.R. Lantos, C.P. Harnik, M. Scott, A.I. 18 deoxyaldosterone 18 hydroxycorticosterone aldosterone radioisotope unclassified drug adrenal gland animal cell article endocrine system nonhuman priority journal rat 18-Hydroxycorticosterone Adrenal Glands Aldosterone Animals Corticosterone Hydrogen-Ion Concentration Liposomes Magnetic Resonance Spectroscopy Male Rats Animalia Samples containing as precursors either 18-hydroxycorticosterone (18-OH-B) in its M form, or this converted to less polar forms at pH 2 (ACM), or M or ACM enclosed in liposomes from adrenal lipids were incubated at pH 7.4, 4.8 or 3.3 in the presence or absence of quartered rat adrenals for 1 and 2 h. Optimal (10%) yields of aldosterone were obtained when (a) ACM was incubated at pH 4.8 and (b) M enclosed in liposomes was suspended in buffer and shaken without enzyme at pH 3.3. When conditions (a) were supplemented with malate and NADP, 16% of ACM was converted to aldosterone. ACM contained 80% of a fraction which, according to 13C NMR spectroscopy, was identical to 18-deoxyaldosterone (18-DAL). Experiments in which radioactivity from corticosterone (B) or M was trapped by radioinert M or 18-DAL disclosed a pathway comprising sequentially B, 18-OH-B, 18-DAL and aldosterone, and the combined evidence of this work, an enzymatic hydroxylation of 18-DAL to aldosterone. © 1985. Fil:Cozza, E.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Burton, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ceballos, N.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00224731_v22_n5_p665_Cozza
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 18 deoxyaldosterone
18 hydroxycorticosterone
aldosterone
radioisotope
unclassified drug
adrenal gland
animal cell
article
endocrine system
nonhuman
priority journal
rat
18-Hydroxycorticosterone
Adrenal Glands
Aldosterone
Animals
Corticosterone
Hydrogen-Ion Concentration
Liposomes
Magnetic Resonance Spectroscopy
Male
Rats
Animalia
spellingShingle 18 deoxyaldosterone
18 hydroxycorticosterone
aldosterone
radioisotope
unclassified drug
adrenal gland
animal cell
article
endocrine system
nonhuman
priority journal
rat
18-Hydroxycorticosterone
Adrenal Glands
Aldosterone
Animals
Corticosterone
Hydrogen-Ion Concentration
Liposomes
Magnetic Resonance Spectroscopy
Male
Rats
Animalia
Cozza, E.N.
Burton, G.
Ceballos, N.R.
Lantos, C.P.
Harnik, M.
Scott, A.I.
Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals
topic_facet 18 deoxyaldosterone
18 hydroxycorticosterone
aldosterone
radioisotope
unclassified drug
adrenal gland
animal cell
article
endocrine system
nonhuman
priority journal
rat
18-Hydroxycorticosterone
Adrenal Glands
Aldosterone
Animals
Corticosterone
Hydrogen-Ion Concentration
Liposomes
Magnetic Resonance Spectroscopy
Male
Rats
Animalia
description Samples containing as precursors either 18-hydroxycorticosterone (18-OH-B) in its M form, or this converted to less polar forms at pH 2 (ACM), or M or ACM enclosed in liposomes from adrenal lipids were incubated at pH 7.4, 4.8 or 3.3 in the presence or absence of quartered rat adrenals for 1 and 2 h. Optimal (10%) yields of aldosterone were obtained when (a) ACM was incubated at pH 4.8 and (b) M enclosed in liposomes was suspended in buffer and shaken without enzyme at pH 3.3. When conditions (a) were supplemented with malate and NADP, 16% of ACM was converted to aldosterone. ACM contained 80% of a fraction which, according to 13C NMR spectroscopy, was identical to 18-deoxyaldosterone (18-DAL). Experiments in which radioactivity from corticosterone (B) or M was trapped by radioinert M or 18-DAL disclosed a pathway comprising sequentially B, 18-OH-B, 18-DAL and aldosterone, and the combined evidence of this work, an enzymatic hydroxylation of 18-DAL to aldosterone. © 1985.
format JOUR
author Cozza, E.N.
Burton, G.
Ceballos, N.R.
Lantos, C.P.
Harnik, M.
Scott, A.I.
author_facet Cozza, E.N.
Burton, G.
Ceballos, N.R.
Lantos, C.P.
Harnik, M.
Scott, A.I.
author_sort Cozza, E.N.
title Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals
title_short Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals
title_full Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals
title_fullStr Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals
title_full_unstemmed Eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals
title_sort eighteen-deoxyaldosterone and other less polar forms of 18-hydroxycorticosterone as aldosterone precursors in rat adrenals
url http://hdl.handle.net/20.500.12110/paper_00224731_v22_n5_p665_Cozza
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AT burtong eighteendeoxyaldosteroneandotherlesspolarformsof18hydroxycorticosteroneasaldosteroneprecursorsinratadrenals
AT ceballosnr eighteendeoxyaldosteroneandotherlesspolarformsof18hydroxycorticosteroneasaldosteroneprecursorsinratadrenals
AT lantoscp eighteendeoxyaldosteroneandotherlesspolarformsof18hydroxycorticosteroneasaldosteroneprecursorsinratadrenals
AT harnikm eighteendeoxyaldosteroneandotherlesspolarformsof18hydroxycorticosteroneasaldosteroneprecursorsinratadrenals
AT scottai eighteendeoxyaldosteroneandotherlesspolarformsof18hydroxycorticosteroneasaldosteroneprecursorsinratadrenals
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