The in vitro metabolism of androgens in rat epididymis during sexual development

The in vitro activity of epididymal 5α-reductase and 3-hydroxy-steroid dehydrogenase was studied in rats during sexual development (ages 15 to 50 days). It was found that total activity (per organ) of 3-hydroxysteroid dehydrogenase reached an absolute maximum in 25 day-old rats. At this age there wa...

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Autores principales: De Larminat, M.A., Cuasnicú, P.S., Blaquier, J.A.
Formato: JOUR
Materias:
3(or 17)beta hydroxysteroid dehydrogenase
androgen
androstanediol h 3
androstanolone c 14
androstanolone h 3
radioisotope
steroid reductase
testosterone c 14
testosterone h 3
unclassified drug
age
animal experiment
article
epididymis
hormone metabolism
infant
male genital system
preliminary communication
rat
sexual development
Animalia
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00224731_v13_n10_p1207_DeLarminat
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00224731_v13_n10_p1207_DeLarminat
record_format dspace
spelling todo:paper_00224731_v13_n10_p1207_DeLarminat2023-10-03T14:33:01Z The in vitro metabolism of androgens in rat epididymis during sexual development De Larminat, M.A. Cuasnicú, P.S. Blaquier, J.A. 3(or 17)beta hydroxysteroid dehydrogenase androgen androstanediol h 3 androstanolone c 14 androstanolone h 3 radioisotope steroid reductase testosterone c 14 testosterone h 3 unclassified drug age animal experiment article epididymis hormone metabolism infant male genital system preliminary communication rat sexual development Animalia The in vitro activity of epididymal 5α-reductase and 3-hydroxy-steroid dehydrogenase was studied in rats during sexual development (ages 15 to 50 days). It was found that total activity (per organ) of 3-hydroxysteroid dehydrogenase reached an absolute maximum in 25 day-old rats. At this age there was also a marked increase in the specific activity (per mg weight) of both enzymes studied. While at all other ages 5α-DHT was the main product from testosterone, in the 25 day-old rat, 5α-androstandiol accounted for 73% of the metabolites formed. The subcellular distribution of both enzymes in the epididymides of adult and prepuberal rats was found to present only minor differences. Kinetic parameters (KM and Vmax) were measured in the subcellular fractions of the immature epididymis. These results indicate the existence of a marked, but transient, shift in the activity of the enzymes which metabolize androgens in the epididymis, towards the formation of 5α-androstandiol during the discrete period between 20 and 30 days of life. © 1980, All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00224731_v13_n10_p1207_DeLarminat
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 3(or 17)beta hydroxysteroid dehydrogenase
androgen
androstanediol h 3
androstanolone c 14
androstanolone h 3
radioisotope
steroid reductase
testosterone c 14
testosterone h 3
unclassified drug
age
animal experiment
article
epididymis
hormone metabolism
infant
male genital system
preliminary communication
rat
sexual development
Animalia
spellingShingle 3(or 17)beta hydroxysteroid dehydrogenase
androgen
androstanediol h 3
androstanolone c 14
androstanolone h 3
radioisotope
steroid reductase
testosterone c 14
testosterone h 3
unclassified drug
age
animal experiment
article
epididymis
hormone metabolism
infant
male genital system
preliminary communication
rat
sexual development
Animalia
De Larminat, M.A.
Cuasnicú, P.S.
Blaquier, J.A.
The in vitro metabolism of androgens in rat epididymis during sexual development
topic_facet 3(or 17)beta hydroxysteroid dehydrogenase
androgen
androstanediol h 3
androstanolone c 14
androstanolone h 3
radioisotope
steroid reductase
testosterone c 14
testosterone h 3
unclassified drug
age
animal experiment
article
epididymis
hormone metabolism
infant
male genital system
preliminary communication
rat
sexual development
Animalia
description The in vitro activity of epididymal 5α-reductase and 3-hydroxy-steroid dehydrogenase was studied in rats during sexual development (ages 15 to 50 days). It was found that total activity (per organ) of 3-hydroxysteroid dehydrogenase reached an absolute maximum in 25 day-old rats. At this age there was also a marked increase in the specific activity (per mg weight) of both enzymes studied. While at all other ages 5α-DHT was the main product from testosterone, in the 25 day-old rat, 5α-androstandiol accounted for 73% of the metabolites formed. The subcellular distribution of both enzymes in the epididymides of adult and prepuberal rats was found to present only minor differences. Kinetic parameters (KM and Vmax) were measured in the subcellular fractions of the immature epididymis. These results indicate the existence of a marked, but transient, shift in the activity of the enzymes which metabolize androgens in the epididymis, towards the formation of 5α-androstandiol during the discrete period between 20 and 30 days of life. © 1980, All rights reserved.
format JOUR
author De Larminat, M.A.
Cuasnicú, P.S.
Blaquier, J.A.
author_facet De Larminat, M.A.
Cuasnicú, P.S.
Blaquier, J.A.
author_sort De Larminat, M.A.
title The in vitro metabolism of androgens in rat epididymis during sexual development
title_short The in vitro metabolism of androgens in rat epididymis during sexual development
title_full The in vitro metabolism of androgens in rat epididymis during sexual development
title_fullStr The in vitro metabolism of androgens in rat epididymis during sexual development
title_full_unstemmed The in vitro metabolism of androgens in rat epididymis during sexual development
title_sort in vitro metabolism of androgens in rat epididymis during sexual development
url http://hdl.handle.net/20.500.12110/paper_00224731_v13_n10_p1207_DeLarminat
work_keys_str_mv AT delarminatma theinvitrometabolismofandrogensinratepididymisduringsexualdevelopment
AT cuasnicups theinvitrometabolismofandrogensinratepididymisduringsexualdevelopment
AT blaquierja theinvitrometabolismofandrogensinratepididymisduringsexualdevelopment
AT delarminatma invitrometabolismofandrogensinratepididymisduringsexualdevelopment
AT cuasnicups invitrometabolismofandrogensinratepididymisduringsexualdevelopment
AT blaquierja invitrometabolismofandrogensinratepididymisduringsexualdevelopment
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