Addition of androgens to cultured hamster epididymis increases zona recognition by immature spermatozoa

The ability of spermatozoa recovered from the successive segments of the hamster epididymis to bind to the zona pellucida was studied and a major increase was found as spermatozoa passed from the proximal to the distal portions of the corpus epididymidis (1.95 compared with 20 spermatozoa bound/egg)...

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Autores principales: Cuasnicu, P.S., Echeverria, F.G., Piazza, A., Blaquier, J.A.
Formato: JOUR
Materias:
amino acid h 3
androgen
androstanolone
cyproterone acetate
unclassified drug
article
drug efficacy
epididymis
in vitro study
male genital system
nonhuman
spermatozoon
zona pellucida
Cricetinae
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00224251_v70_n2_p541_Cuasnicu
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00224251_v70_n2_p541_Cuasnicu
record_format dspace
spelling todo:paper_00224251_v70_n2_p541_Cuasnicu2023-10-03T14:32:52Z Addition of androgens to cultured hamster epididymis increases zona recognition by immature spermatozoa Cuasnicu, P.S. Echeverria, F.G. Piazza, A. Blaquier, J.A. amino acid h 3 androgen androstanolone cyproterone acetate unclassified drug article drug efficacy epididymis in vitro study male genital system nonhuman spermatozoon zona pellucida Cricetinae The ability of spermatozoa recovered from the successive segments of the hamster epididymis to bind to the zona pellucida was studied and a major increase was found as spermatozoa passed from the proximal to the distal portions of the corpus epididymidis (1.95 compared with 20 spermatozoa bound/egg). Tubules from the proximal epididymis were cultured in conditions which preserved the motility of the contained spermatozoa for 48-72 h. Addition of 2 μM-5α-DHT to the culture medium for 17 h stimulated the incorporation of 3H-labelled amino acids into several protein bands whose mobility in polyacrylamide gel electrophoresis was coincident with those of glycoproteins EP1-EP6, previously identified as androgen-dependent in the hamster epididymis in vivo. Examination of the material extracted from washed spermatozoa with 0.5 M-NaCl revealed the presence of radioactive proteins on spermatozoa. The zona-binding ability of spermatozoa from androgen-treated cultured proximal corpus tubules was significantly increased (P < 0.001) as was the no. of spermatozoa/egg (5.51) compared with the value for control cultures (0.87 spermatozoa/egg). We suggest that androgen-dependent epididymal secretory proteins that associate with spermatozoa might participate in the formation or activation of a site for zona pellucida recognition in the sperm surface. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00224251_v70_n2_p541_Cuasnicu
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic amino acid h 3
androgen
androstanolone
cyproterone acetate
unclassified drug
article
drug efficacy
epididymis
in vitro study
male genital system
nonhuman
spermatozoon
zona pellucida
Cricetinae
spellingShingle amino acid h 3
androgen
androstanolone
cyproterone acetate
unclassified drug
article
drug efficacy
epididymis
in vitro study
male genital system
nonhuman
spermatozoon
zona pellucida
Cricetinae
Cuasnicu, P.S.
Echeverria, F.G.
Piazza, A.
Blaquier, J.A.
Addition of androgens to cultured hamster epididymis increases zona recognition by immature spermatozoa
topic_facet amino acid h 3
androgen
androstanolone
cyproterone acetate
unclassified drug
article
drug efficacy
epididymis
in vitro study
male genital system
nonhuman
spermatozoon
zona pellucida
Cricetinae
description The ability of spermatozoa recovered from the successive segments of the hamster epididymis to bind to the zona pellucida was studied and a major increase was found as spermatozoa passed from the proximal to the distal portions of the corpus epididymidis (1.95 compared with 20 spermatozoa bound/egg). Tubules from the proximal epididymis were cultured in conditions which preserved the motility of the contained spermatozoa for 48-72 h. Addition of 2 μM-5α-DHT to the culture medium for 17 h stimulated the incorporation of 3H-labelled amino acids into several protein bands whose mobility in polyacrylamide gel electrophoresis was coincident with those of glycoproteins EP1-EP6, previously identified as androgen-dependent in the hamster epididymis in vivo. Examination of the material extracted from washed spermatozoa with 0.5 M-NaCl revealed the presence of radioactive proteins on spermatozoa. The zona-binding ability of spermatozoa from androgen-treated cultured proximal corpus tubules was significantly increased (P < 0.001) as was the no. of spermatozoa/egg (5.51) compared with the value for control cultures (0.87 spermatozoa/egg). We suggest that androgen-dependent epididymal secretory proteins that associate with spermatozoa might participate in the formation or activation of a site for zona pellucida recognition in the sperm surface.
format JOUR
author Cuasnicu, P.S.
Echeverria, F.G.
Piazza, A.
Blaquier, J.A.
author_facet Cuasnicu, P.S.
Echeverria, F.G.
Piazza, A.
Blaquier, J.A.
author_sort Cuasnicu, P.S.
title Addition of androgens to cultured hamster epididymis increases zona recognition by immature spermatozoa
title_short Addition of androgens to cultured hamster epididymis increases zona recognition by immature spermatozoa
title_full Addition of androgens to cultured hamster epididymis increases zona recognition by immature spermatozoa
title_fullStr Addition of androgens to cultured hamster epididymis increases zona recognition by immature spermatozoa
title_full_unstemmed Addition of androgens to cultured hamster epididymis increases zona recognition by immature spermatozoa
title_sort addition of androgens to cultured hamster epididymis increases zona recognition by immature spermatozoa
url http://hdl.handle.net/20.500.12110/paper_00224251_v70_n2_p541_Cuasnicu
work_keys_str_mv AT cuasnicups additionofandrogenstoculturedhamsterepididymisincreaseszonarecognitionbyimmaturespermatozoa
AT echeverriafg additionofandrogenstoculturedhamsterepididymisincreaseszonarecognitionbyimmaturespermatozoa
AT piazzaa additionofandrogenstoculturedhamsterepididymisincreaseszonarecognitionbyimmaturespermatozoa
AT blaquierja additionofandrogenstoculturedhamsterepididymisincreaseszonarecognitionbyimmaturespermatozoa
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