Identification of androgen-dependent glycoproteins in the hamster epididymis and their association with spermatozoa

Androgen-dependent epididymal proteins were investigated in the hamster. The stimulation of labelled amino acid incorporation, as well as the colour intensity of bands stained with Coomassie Blue after electrophoresis of the epididymal cytosol from castrated animals with and without androgen replace...

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Autores principales: Gonzalez Echeverria, F.M., Cuasnicu, P.S., Blaquier, J.A.
Formato: JOUR
Materias:
androgen
glycoprotein
radioisotope
amino acid c 14
amino acid h 3
animal experiment
endocrine system
epididymis
male genital system
spermatozoon
Amino Acids
Animal
Castration
Electrophoresis, Polyacrylamide Gel
Epididymis
Glycoproteins
Hamsters
Male
Mesocricetus
Molecular Weight
Spermatozoa
Support, Non-U.S. Gov't
Testosterone
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00224251_v64_n1_p1_GonzalezEcheverria
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00224251_v64_n1_p1_GonzalezEcheverria
record_format dspace
spelling todo:paper_00224251_v64_n1_p1_GonzalezEcheverria2023-10-03T14:32:52Z Identification of androgen-dependent glycoproteins in the hamster epididymis and their association with spermatozoa Gonzalez Echeverria, F.M. Cuasnicu, P.S. Blaquier, J.A. androgen glycoprotein radioisotope amino acid c 14 amino acid h 3 animal experiment endocrine system epididymis male genital system spermatozoon Amino Acids Animal Castration Electrophoresis, Polyacrylamide Gel Epididymis Glycoproteins Hamsters Male Mesocricetus Molecular Weight Spermatozoa Support, Non-U.S. Gov't Testosterone Androgen-dependent epididymal proteins were investigated in the hamster. The stimulation of labelled amino acid incorporation, as well as the colour intensity of bands stained with Coomassie Blue after electrophoresis of the epididymal cytosol from castrated animals with and without androgen replacement, were used as semi-quantitative criteria for evaluation. These techniques allowed the identification of 6 androgen-sensitive bands (EP) with the following relative electrophoretic mobilities with respect to albumin: EP1 = 0.8; EP2 = 1.11; EP3 = 1.21; EP4 = 1.31; EP5 = 1.52; EP6 = 1.63. The proteins EP1, EP3 and EP4 were also found in fluid from the cauda epididymidis. Extraction of spermatozoa from the distal corpus and cauda epididymidis with 0.25 and 0.5 M-NaCl yielded appreciable amounts of EP2 and EP3 but these bands were not detected in extracts of spermatozoa from proximal segments. The approximate molecular weights were 61 400 for EP1, 42 500 for EP2, 23 800 for EP3, 20 400 for EP4, 26 100 for EP5 and 41 000 for EP6. All bands stained as glycoproteins with periodic acid-Schiff reagent. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00224251_v64_n1_p1_GonzalezEcheverria
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic androgen
glycoprotein
radioisotope
amino acid c 14
amino acid h 3
animal experiment
endocrine system
epididymis
male genital system
spermatozoon
Amino Acids
Animal
Castration
Electrophoresis, Polyacrylamide Gel
Epididymis
Glycoproteins
Hamsters
Male
Mesocricetus
Molecular Weight
Spermatozoa
Support, Non-U.S. Gov't
Testosterone
spellingShingle androgen
glycoprotein
radioisotope
amino acid c 14
amino acid h 3
animal experiment
endocrine system
epididymis
male genital system
spermatozoon
Amino Acids
Animal
Castration
Electrophoresis, Polyacrylamide Gel
Epididymis
Glycoproteins
Hamsters
Male
Mesocricetus
Molecular Weight
Spermatozoa
Support, Non-U.S. Gov't
Testosterone
Gonzalez Echeverria, F.M.
Cuasnicu, P.S.
Blaquier, J.A.
Identification of androgen-dependent glycoproteins in the hamster epididymis and their association with spermatozoa
topic_facet androgen
glycoprotein
radioisotope
amino acid c 14
amino acid h 3
animal experiment
endocrine system
epididymis
male genital system
spermatozoon
Amino Acids
Animal
Castration
Electrophoresis, Polyacrylamide Gel
Epididymis
Glycoproteins
Hamsters
Male
Mesocricetus
Molecular Weight
Spermatozoa
Support, Non-U.S. Gov't
Testosterone
description Androgen-dependent epididymal proteins were investigated in the hamster. The stimulation of labelled amino acid incorporation, as well as the colour intensity of bands stained with Coomassie Blue after electrophoresis of the epididymal cytosol from castrated animals with and without androgen replacement, were used as semi-quantitative criteria for evaluation. These techniques allowed the identification of 6 androgen-sensitive bands (EP) with the following relative electrophoretic mobilities with respect to albumin: EP1 = 0.8; EP2 = 1.11; EP3 = 1.21; EP4 = 1.31; EP5 = 1.52; EP6 = 1.63. The proteins EP1, EP3 and EP4 were also found in fluid from the cauda epididymidis. Extraction of spermatozoa from the distal corpus and cauda epididymidis with 0.25 and 0.5 M-NaCl yielded appreciable amounts of EP2 and EP3 but these bands were not detected in extracts of spermatozoa from proximal segments. The approximate molecular weights were 61 400 for EP1, 42 500 for EP2, 23 800 for EP3, 20 400 for EP4, 26 100 for EP5 and 41 000 for EP6. All bands stained as glycoproteins with periodic acid-Schiff reagent.
format JOUR
author Gonzalez Echeverria, F.M.
Cuasnicu, P.S.
Blaquier, J.A.
author_facet Gonzalez Echeverria, F.M.
Cuasnicu, P.S.
Blaquier, J.A.
author_sort Gonzalez Echeverria, F.M.
title Identification of androgen-dependent glycoproteins in the hamster epididymis and their association with spermatozoa
title_short Identification of androgen-dependent glycoproteins in the hamster epididymis and their association with spermatozoa
title_full Identification of androgen-dependent glycoproteins in the hamster epididymis and their association with spermatozoa
title_fullStr Identification of androgen-dependent glycoproteins in the hamster epididymis and their association with spermatozoa
title_full_unstemmed Identification of androgen-dependent glycoproteins in the hamster epididymis and their association with spermatozoa
title_sort identification of androgen-dependent glycoproteins in the hamster epididymis and their association with spermatozoa
url http://hdl.handle.net/20.500.12110/paper_00224251_v64_n1_p1_GonzalezEcheverria
work_keys_str_mv AT gonzalezecheverriafm identificationofandrogendependentglycoproteinsinthehamsterepididymisandtheirassociationwithspermatozoa
AT cuasnicups identificationofandrogendependentglycoproteinsinthehamsterepididymisandtheirassociationwithspermatozoa
AT blaquierja identificationofandrogendependentglycoproteinsinthehamsterepididymisandtheirassociationwithspermatozoa
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