Cell wall variability in the green seaweed codium vermilara (bryopsidales chlorophyta) from the argentine coast

Cell wall chemistry in the coencocytic green seaweed Codium vermilara (Olivi) Delle Chiaje (Bryopsidales, Chlorophyta) is well understood. These cell walls are composed of major amounts of neutral β-(1→4)-D-mannans (Mn), sulfated polysaccharides (SPs), which include pyranosic arabinan sulfates (ArpS...

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Autores principales: Fernández, P.V., Ciancia, M., Estevez, J.M.
Formato: JOUR
Materias:
β-mannans
Cell wall
Codium vermilara
Green seaweed
Sulfated polysaccharides
Temporal and geographic variation
Caulerpales
Chlorophyta
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00223646_v47_n4_p802_Fernandez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_00223646_v47_n4_p802_Fernandez2023-10-03T14:32:04Z Cell wall variability in the green seaweed codium vermilara (bryopsidales chlorophyta) from the argentine coast Fernández, P.V. Ciancia, M. Estevez, J.M. β-mannans Cell wall Codium vermilara Green seaweed Sulfated polysaccharides Temporal and geographic variation Caulerpales Chlorophyta Codium vermilara Cell wall chemistry in the coencocytic green seaweed Codium vermilara (Olivi) Delle Chiaje (Bryopsidales, Chlorophyta) is well understood. These cell walls are composed of major amounts of neutral β-(1→4)-D-mannans (Mn), sulfated polysaccharides (SPs), which include pyranosic arabinan sulfates (ArpS), pyruvylated galactan sulfates (pGaS), and mannan sulfates (MnS); also minor amounts of O-glycoproteins are present. In this study, cell wall samples of C. vermilara were investigated with regard to their monosaccharide composition and infrared spectra (using Fourier transform infrared spectroscopy coupled to principal component [FTIR-PC] analysis). Samples from three different populations of C. vermilara from the Argentine coast showed: (i) an important variation in the relative arabinan content, which increases from north to south, and (ii) a measurable degree of cell wall variability in the sulfate distribution between the different sulfated polysaccharides, independent of the amount of each polysaccharide present and of total sulfate content. When cell wall composition was analyzed over three consecutive years in a single geographic location, the quantity of Mn and overall sulfate content on SPs remained constant, whereas the pGaS:ArpS molar ratio changed over the time. Besides, similar cell wall composition was found between actively growing and resting zones of the thallus, suggesting that cell wall composition is independent of growth stage and development. Overall, these results suggest that C. vermilara has developed a mechanism to adjust the total level of cell wall sulfation by modulating the ArpS:pGaS:MnS molar ratio and also by adjusting the sulfation level in each type of polymer, whereas nonsulfated Mn, as the main structural polysaccharide, did not change over the time or growing stage. © 2011 Phycological Society of America. Fil:Fernández, P.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ciancia, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Estevez, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00223646_v47_n4_p802_Fernandez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic β-mannans
Cell wall
Codium vermilara
Green seaweed
Sulfated polysaccharides
Temporal and geographic variation
Caulerpales
Chlorophyta
Codium vermilara
spellingShingle β-mannans
Cell wall
Codium vermilara
Green seaweed
Sulfated polysaccharides
Temporal and geographic variation
Caulerpales
Chlorophyta
Codium vermilara
Fernández, P.V.
Ciancia, M.
Estevez, J.M.
Cell wall variability in the green seaweed codium vermilara (bryopsidales chlorophyta) from the argentine coast
topic_facet β-mannans
Cell wall
Codium vermilara
Green seaweed
Sulfated polysaccharides
Temporal and geographic variation
Caulerpales
Chlorophyta
Codium vermilara
description Cell wall chemistry in the coencocytic green seaweed Codium vermilara (Olivi) Delle Chiaje (Bryopsidales, Chlorophyta) is well understood. These cell walls are composed of major amounts of neutral β-(1→4)-D-mannans (Mn), sulfated polysaccharides (SPs), which include pyranosic arabinan sulfates (ArpS), pyruvylated galactan sulfates (pGaS), and mannan sulfates (MnS); also minor amounts of O-glycoproteins are present. In this study, cell wall samples of C. vermilara were investigated with regard to their monosaccharide composition and infrared spectra (using Fourier transform infrared spectroscopy coupled to principal component [FTIR-PC] analysis). Samples from three different populations of C. vermilara from the Argentine coast showed: (i) an important variation in the relative arabinan content, which increases from north to south, and (ii) a measurable degree of cell wall variability in the sulfate distribution between the different sulfated polysaccharides, independent of the amount of each polysaccharide present and of total sulfate content. When cell wall composition was analyzed over three consecutive years in a single geographic location, the quantity of Mn and overall sulfate content on SPs remained constant, whereas the pGaS:ArpS molar ratio changed over the time. Besides, similar cell wall composition was found between actively growing and resting zones of the thallus, suggesting that cell wall composition is independent of growth stage and development. Overall, these results suggest that C. vermilara has developed a mechanism to adjust the total level of cell wall sulfation by modulating the ArpS:pGaS:MnS molar ratio and also by adjusting the sulfation level in each type of polymer, whereas nonsulfated Mn, as the main structural polysaccharide, did not change over the time or growing stage. © 2011 Phycological Society of America.
format JOUR
author Fernández, P.V.
Ciancia, M.
Estevez, J.M.
author_facet Fernández, P.V.
Ciancia, M.
Estevez, J.M.
author_sort Fernández, P.V.
title Cell wall variability in the green seaweed codium vermilara (bryopsidales chlorophyta) from the argentine coast
title_short Cell wall variability in the green seaweed codium vermilara (bryopsidales chlorophyta) from the argentine coast
title_full Cell wall variability in the green seaweed codium vermilara (bryopsidales chlorophyta) from the argentine coast
title_fullStr Cell wall variability in the green seaweed codium vermilara (bryopsidales chlorophyta) from the argentine coast
title_full_unstemmed Cell wall variability in the green seaweed codium vermilara (bryopsidales chlorophyta) from the argentine coast
title_sort cell wall variability in the green seaweed codium vermilara (bryopsidales chlorophyta) from the argentine coast
url http://hdl.handle.net/20.500.12110/paper_00223646_v47_n4_p802_Fernandez
work_keys_str_mv AT fernandezpv cellwallvariabilityinthegreenseaweedcodiumvermilarabryopsidaleschlorophytafromtheargentinecoast
AT cianciam cellwallvariabilityinthegreenseaweedcodiumvermilarabryopsidaleschlorophytafromtheargentinecoast
AT estevezjm cellwallvariabilityinthegreenseaweedcodiumvermilarabryopsidaleschlorophytafromtheargentinecoast
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