Differences in polysaccharide structure between calcified and uncalcified segments in the coralline calliarthron cheilosporioides (corallinales, rhodophyta)

The articulated coralline Calliarthron cheilosporioides Manza produces segmented fronds composed of calcified segments (intergenicula) separated by uncalcified joints (genicula), which allow fronds to bend and reorient under breaking waves in the wave-swept intertidal zone. Genicula are formed when...

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Autores principales: Martone, P.T., Navarro, D.A., Stortz, C.A., Estevez, J.M.
Formato: JOUR
Materias:
Agarans
Calliarthron cheilosporioides
Cell wall
Coralline algae
Galactans
Genicula
Intergenicula
Red seaweed
algae
Corallinales
Rhodophyta
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00223646_v46_n3_p507_Martone
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_00223646_v46_n3_p507_Martone2023-10-03T14:32:03Z Differences in polysaccharide structure between calcified and uncalcified segments in the coralline calliarthron cheilosporioides (corallinales, rhodophyta) Martone, P.T. Navarro, D.A. Stortz, C.A. Estevez, J.M. Agarans Calliarthron cheilosporioides Cell wall Coralline algae Galactans Genicula Intergenicula Red seaweed algae Calliarthron cheilosporioides Corallinales Genicula Rhodophyta The articulated coralline Calliarthron cheilosporioides Manza produces segmented fronds composed of calcified segments (intergenicula) separated by uncalcified joints (genicula), which allow fronds to bend and reorient under breaking waves in the wave-swept intertidal zone. Genicula are formed when calcified cells decalcify and restructure to create flexible tissue. The present study has identified important differences in the main agaran disaccharidic repeating units [→3)-β- d-Gal. p (1→ 4)-α- l-Gal. p(1→] synthesized by genicular and intergenicular segments. Based on chemical and spectroscopical analyses, we report that genicular cells from C. cheilosporioides biosynthesize a highly methoxylated galactan at C-6 position with low levels of branching with xylose side stubs on C-6 of the [→3)-β- d-Gal. p (1→] units, whereas intergenicular segments produce xylogalactans with high levels of xylose and low levels of 6- O-methyl β- d-Gal units. These data suggest that, during genicular development, xylosyl branched, 3-linked β- d-Gal. p units present in the xylogalactan backbones from intergenicular walls are mostly replaced by 6- O-methyl. -d-galactose units. We speculate that this structural shift is a consequence of a putative and specific methoxyl transferase that blocks the xylosylation on C-6 of the 3-linked β- d-Gal. p units. Changes in galactan substitutions may contribute to the distinct mechanical properties of genicula and may lend insight into the calcification process in coralline algae. © 2010 Phycological Society of America. Fil:Navarro, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Stortz, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Estevez, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00223646_v46_n3_p507_Martone
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Agarans
Calliarthron cheilosporioides
Cell wall
Coralline algae
Galactans
Genicula
Intergenicula
Red seaweed
algae
Calliarthron cheilosporioides
Corallinales
Genicula
Rhodophyta
spellingShingle Agarans
Calliarthron cheilosporioides
Cell wall
Coralline algae
Galactans
Genicula
Intergenicula
Red seaweed
algae
Calliarthron cheilosporioides
Corallinales
Genicula
Rhodophyta
Martone, P.T.
Navarro, D.A.
Stortz, C.A.
Estevez, J.M.
Differences in polysaccharide structure between calcified and uncalcified segments in the coralline calliarthron cheilosporioides (corallinales, rhodophyta)
topic_facet Agarans
Calliarthron cheilosporioides
Cell wall
Coralline algae
Galactans
Genicula
Intergenicula
Red seaweed
algae
Calliarthron cheilosporioides
Corallinales
Genicula
Rhodophyta
description The articulated coralline Calliarthron cheilosporioides Manza produces segmented fronds composed of calcified segments (intergenicula) separated by uncalcified joints (genicula), which allow fronds to bend and reorient under breaking waves in the wave-swept intertidal zone. Genicula are formed when calcified cells decalcify and restructure to create flexible tissue. The present study has identified important differences in the main agaran disaccharidic repeating units [→3)-β- d-Gal. p (1→ 4)-α- l-Gal. p(1→] synthesized by genicular and intergenicular segments. Based on chemical and spectroscopical analyses, we report that genicular cells from C. cheilosporioides biosynthesize a highly methoxylated galactan at C-6 position with low levels of branching with xylose side stubs on C-6 of the [→3)-β- d-Gal. p (1→] units, whereas intergenicular segments produce xylogalactans with high levels of xylose and low levels of 6- O-methyl β- d-Gal units. These data suggest that, during genicular development, xylosyl branched, 3-linked β- d-Gal. p units present in the xylogalactan backbones from intergenicular walls are mostly replaced by 6- O-methyl. -d-galactose units. We speculate that this structural shift is a consequence of a putative and specific methoxyl transferase that blocks the xylosylation on C-6 of the 3-linked β- d-Gal. p units. Changes in galactan substitutions may contribute to the distinct mechanical properties of genicula and may lend insight into the calcification process in coralline algae. © 2010 Phycological Society of America.
format JOUR
author Martone, P.T.
Navarro, D.A.
Stortz, C.A.
Estevez, J.M.
author_facet Martone, P.T.
Navarro, D.A.
Stortz, C.A.
Estevez, J.M.
author_sort Martone, P.T.
title Differences in polysaccharide structure between calcified and uncalcified segments in the coralline calliarthron cheilosporioides (corallinales, rhodophyta)
title_short Differences in polysaccharide structure between calcified and uncalcified segments in the coralline calliarthron cheilosporioides (corallinales, rhodophyta)
title_full Differences in polysaccharide structure between calcified and uncalcified segments in the coralline calliarthron cheilosporioides (corallinales, rhodophyta)
title_fullStr Differences in polysaccharide structure between calcified and uncalcified segments in the coralline calliarthron cheilosporioides (corallinales, rhodophyta)
title_full_unstemmed Differences in polysaccharide structure between calcified and uncalcified segments in the coralline calliarthron cheilosporioides (corallinales, rhodophyta)
title_sort differences in polysaccharide structure between calcified and uncalcified segments in the coralline calliarthron cheilosporioides (corallinales, rhodophyta)
url http://hdl.handle.net/20.500.12110/paper_00223646_v46_n3_p507_Martone
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