Structure analysis of two Toxoplasma gondii and Neospora caninum satellite DNA families and evolution of their common monomeric sequence

A family of repetitive DNA elements of approximately 350 bp - Sat350 - that are members of Toxoplasma gondii satellite DNA was further analyzed. Sequence analysis identified at least three distinct repeat types within this family, called types A, B, and C. B repeats were divided into the subtypes B1...

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Autores principales:	Clemente, M., De Miguel, N., Lia, V.V., Matrajt, M., Angel, S.O.
Formato:	JOUR
Materias:	EST Heterochromatin Neospora caninum Satellite DNA Tandem repeat Toxoplasma gondii monomer primer DNA repetitive DNA satellite DNA article controlled study correlation analysis data base DNA flanking region DNA sequence gene amplification gene expression gene identification gene structure genetic analysis genetic conservation genome molecular evolution molecular phylogeny multigene family nonhuman nucleotide sequence polymerase chain reaction protein assembly sequence analysis Southern blotting structure analysis telomere Animals Bacteriophages Cloning, Molecular Contig Mapping DNA, Satellite Evolution, Molecular Expressed Sequence Tags Neospora Phylogeny Sequence Alignment Sequence Analysis, DNA Toxoplasma Protozoa
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00222844_v58_n5_p557_Clemente
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_00222844_v58_n5_p557_Clemente2023-10-03T14:30:25Z Structure analysis of two Toxoplasma gondii and Neospora caninum satellite DNA families and evolution of their common monomeric sequence Clemente, M. De Miguel, N. Lia, V.V. Matrajt, M. Angel, S.O. EST Heterochromatin Neospora caninum Satellite DNA Tandem repeat Toxoplasma gondii monomer primer DNA repetitive DNA satellite DNA article controlled study correlation analysis data base DNA flanking region DNA sequence gene amplification gene expression gene identification gene structure genetic analysis genetic conservation genome molecular evolution molecular phylogeny multigene family Neospora caninum nonhuman nucleotide sequence polymerase chain reaction protein assembly sequence analysis Southern blotting structure analysis telomere Toxoplasma gondii Animals Bacteriophages Cloning, Molecular Contig Mapping DNA, Satellite Evolution, Molecular Expressed Sequence Tags Neospora Phylogeny Sequence Alignment Sequence Analysis, DNA Toxoplasma Neospora caninum Protozoa Toxoplasma Toxoplasma gondii A family of repetitive DNA elements of approximately 350 bp - Sat350 - that are members of Toxoplasma gondii satellite DNA was further analyzed. Sequence analysis identified at least three distinct repeat types within this family, called types A, B, and C. B repeats were divided into the subtypes B1 and B2. A search for internal repetitions within this family permitted the identification of conserved regions and the design of PCR primers that amplify almost all these repetitive elements. These primers amplified the expected 350-bp repeats and a novel 680-bp repetitive element (Sat680) related to this family. Two additional tandemly repeated high-order structures corresponding to this satellite DNA family were found by searching the Toxoplasma genome database with these sequences. These studies were confirmed by sequence analysis and identified: (1) an arrangement of AB1CB2 350-bp repeats and (2) an arrangement of two 350-bp-like repeats, resulting in a 680-bp monomer. Sequence comparison and phylogenetic analysis indicated that both high-order structures may have originated from the same ancestral 350-bp repeat. PCR amplification, sequence analysis and Southern blot showed that similar high-order structures were also found in the Toxoplasma-sister taxon Neospora caninum. The Toxoplasma genome database (http://ToxoDB.org) permitted the assembly of a contig harboring Sat350 elements at one end and a long nonrepetitive DNA sequence flanking this satellite DNA. The region bordering the Sat350 repeats contained two differentially expressed sequence-related regions and interstitial telomeric sequences. Fil:Clemente, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lia, V.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Matrajt, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Angel, S.O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00222844_v58_n5_p557_Clemente
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	EST Heterochromatin Neospora caninum Satellite DNA Tandem repeat Toxoplasma gondii monomer primer DNA repetitive DNA satellite DNA article controlled study correlation analysis data base DNA flanking region DNA sequence gene amplification gene expression gene identification gene structure genetic analysis genetic conservation genome molecular evolution molecular phylogeny multigene family Neospora caninum nonhuman nucleotide sequence polymerase chain reaction protein assembly sequence analysis Southern blotting structure analysis telomere Toxoplasma gondii Animals Bacteriophages Cloning, Molecular Contig Mapping DNA, Satellite Evolution, Molecular Expressed Sequence Tags Neospora Phylogeny Sequence Alignment Sequence Analysis, DNA Toxoplasma Neospora caninum Protozoa Toxoplasma Toxoplasma gondii
spellingShingle	EST Heterochromatin Neospora caninum Satellite DNA Tandem repeat Toxoplasma gondii monomer primer DNA repetitive DNA satellite DNA article controlled study correlation analysis data base DNA flanking region DNA sequence gene amplification gene expression gene identification gene structure genetic analysis genetic conservation genome molecular evolution molecular phylogeny multigene family Neospora caninum nonhuman nucleotide sequence polymerase chain reaction protein assembly sequence analysis Southern blotting structure analysis telomere Toxoplasma gondii Animals Bacteriophages Cloning, Molecular Contig Mapping DNA, Satellite Evolution, Molecular Expressed Sequence Tags Neospora Phylogeny Sequence Alignment Sequence Analysis, DNA Toxoplasma Neospora caninum Protozoa Toxoplasma Toxoplasma gondii Clemente, M. De Miguel, N. Lia, V.V. Matrajt, M. Angel, S.O. Structure analysis of two Toxoplasma gondii and Neospora caninum satellite DNA families and evolution of their common monomeric sequence
topic_facet	EST Heterochromatin Neospora caninum Satellite DNA Tandem repeat Toxoplasma gondii monomer primer DNA repetitive DNA satellite DNA article controlled study correlation analysis data base DNA flanking region DNA sequence gene amplification gene expression gene identification gene structure genetic analysis genetic conservation genome molecular evolution molecular phylogeny multigene family Neospora caninum nonhuman nucleotide sequence polymerase chain reaction protein assembly sequence analysis Southern blotting structure analysis telomere Toxoplasma gondii Animals Bacteriophages Cloning, Molecular Contig Mapping DNA, Satellite Evolution, Molecular Expressed Sequence Tags Neospora Phylogeny Sequence Alignment Sequence Analysis, DNA Toxoplasma Neospora caninum Protozoa Toxoplasma Toxoplasma gondii
description	A family of repetitive DNA elements of approximately 350 bp - Sat350 - that are members of Toxoplasma gondii satellite DNA was further analyzed. Sequence analysis identified at least three distinct repeat types within this family, called types A, B, and C. B repeats were divided into the subtypes B1 and B2. A search for internal repetitions within this family permitted the identification of conserved regions and the design of PCR primers that amplify almost all these repetitive elements. These primers amplified the expected 350-bp repeats and a novel 680-bp repetitive element (Sat680) related to this family. Two additional tandemly repeated high-order structures corresponding to this satellite DNA family were found by searching the Toxoplasma genome database with these sequences. These studies were confirmed by sequence analysis and identified: (1) an arrangement of AB1CB2 350-bp repeats and (2) an arrangement of two 350-bp-like repeats, resulting in a 680-bp monomer. Sequence comparison and phylogenetic analysis indicated that both high-order structures may have originated from the same ancestral 350-bp repeat. PCR amplification, sequence analysis and Southern blot showed that similar high-order structures were also found in the Toxoplasma-sister taxon Neospora caninum. The Toxoplasma genome database (http://ToxoDB.org) permitted the assembly of a contig harboring Sat350 elements at one end and a long nonrepetitive DNA sequence flanking this satellite DNA. The region bordering the Sat350 repeats contained two differentially expressed sequence-related regions and interstitial telomeric sequences.
format	JOUR
author	Clemente, M. De Miguel, N. Lia, V.V. Matrajt, M. Angel, S.O.
author_facet	Clemente, M. De Miguel, N. Lia, V.V. Matrajt, M. Angel, S.O.
author_sort	Clemente, M.
title	Structure analysis of two Toxoplasma gondii and Neospora caninum satellite DNA families and evolution of their common monomeric sequence
title_short	Structure analysis of two Toxoplasma gondii and Neospora caninum satellite DNA families and evolution of their common monomeric sequence
title_full	Structure analysis of two Toxoplasma gondii and Neospora caninum satellite DNA families and evolution of their common monomeric sequence
title_fullStr	Structure analysis of two Toxoplasma gondii and Neospora caninum satellite DNA families and evolution of their common monomeric sequence
title_full_unstemmed	Structure analysis of two Toxoplasma gondii and Neospora caninum satellite DNA families and evolution of their common monomeric sequence
title_sort	structure analysis of two toxoplasma gondii and neospora caninum satellite dna families and evolution of their common monomeric sequence
url	http://hdl.handle.net/20.500.12110/paper_00222844_v58_n5_p557_Clemente
work_keys_str_mv	AT clementem structureanalysisoftwotoxoplasmagondiiandneosporacaninumsatellitednafamiliesandevolutionoftheircommonmonomericsequence AT demigueln structureanalysisoftwotoxoplasmagondiiandneosporacaninumsatellitednafamiliesandevolutionoftheircommonmonomericsequence AT liavv structureanalysisoftwotoxoplasmagondiiandneosporacaninumsatellitednafamiliesandevolutionoftheircommonmonomericsequence AT matrajtm structureanalysisoftwotoxoplasmagondiiandneosporacaninumsatellitednafamiliesandevolutionoftheircommonmonomericsequence AT angelso structureanalysisoftwotoxoplasmagondiiandneosporacaninumsatellitednafamiliesandevolutionoftheircommonmonomericsequence
_version_	1807315994249527296

Structure analysis of two Toxoplasma gondii and Neospora caninum satellite DNA families and evolution of their common monomeric sequence

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