Multiparametric Fluorescence Detection of Early Stages in the Amyloid Protein Aggregation of Pyrene-labeled α-Synuclein
The aggregation of α-synuclein, a presynaptic protein, has an important role in the etiology of Parkinson's disease. Oligomers or protofibrils adopting the cross-β-sheet structure characteristic of fibrillating amyloid proteins are presumed to be the primary cytotoxic species. Current technique...
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todo:paper_00222836_v378_n5_p1064_Thirunavukkuarasu2023-10-03T14:30:21Z Multiparametric Fluorescence Detection of Early Stages in the Amyloid Protein Aggregation of Pyrene-labeled α-Synuclein Thirunavukkuarasu, S. Jares-Erijman, E.A. Jovin, T.M. fibrillization flourescence anisotropy neurodegenerative oligomers Parkinson's disease alpha synuclein pyrene anisotropy article fluorescence human in vivo study kinetics Parkinson disease point mutation priority journal protein aggregation protein analysis alpha-Synuclein Amino Acid Sequence Amyloid Anisotropy Fluorescent Dyes Humans Molecular Sequence Data Mutagenesis, Site-Directed Parkinson Disease Protein Folding Protein Structure, Quaternary Pyrenes Spectrometry, Fluorescence The aggregation of α-synuclein, a presynaptic protein, has an important role in the etiology of Parkinson's disease. Oligomers or protofibrils adopting the cross-β-sheet structure characteristic of fibrillating amyloid proteins are presumed to be the primary cytotoxic species. Current techniques for monitoring the kinetics of α-synuclein aggregation based on fluorescent dyes such as Thioflavin-T and Congo red detect only the terminal fibrillar species, are discontinuous and notoriously irreproducible. We have devised a new fluorescence aggregation assay that is continuous and provides a large set of fluorescence parameters sensitive to the presence of oligomeric intermediates as well as fibrils. The approach involves tagging functionally neutral Ala-to-Cys variants of α-synuclein with the long-lifetime fluorophore pyrene. Upon induction of aggregation at 37 °C, the entire family of steady-state descriptors of pyrene emission (monomer intensity, solvent polarity ratio (II/IIII), and anisotropy; and excimer intensity) change dramatically, particularly during the early stages in which oligomeric intermediates form and evolve. The pyrene probe senses a progressive decrease in polarity, an increase in molecular mass and close intermolecular association in a manner dependent on position in the sequence and the presence of point mutations. The time-resolved decays (0-160 ns) of intensity and anisotropy exhibited complex, characteristic features. The new assay constitutes a convenient platform for the high-throughput screening of agents useful in the diagnosis and therapy of Parkinson's disease as well as in basic investigations. © 2008 Elsevier Ltd. All rights reserved. Fil:Jares-Erijman, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00222836_v378_n5_p1064_Thirunavukkuarasu |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
fibrillization flourescence anisotropy neurodegenerative oligomers Parkinson's disease alpha synuclein pyrene anisotropy article fluorescence human in vivo study kinetics Parkinson disease point mutation priority journal protein aggregation protein analysis alpha-Synuclein Amino Acid Sequence Amyloid Anisotropy Fluorescent Dyes Humans Molecular Sequence Data Mutagenesis, Site-Directed Parkinson Disease Protein Folding Protein Structure, Quaternary Pyrenes Spectrometry, Fluorescence |
| spellingShingle |
fibrillization flourescence anisotropy neurodegenerative oligomers Parkinson's disease alpha synuclein pyrene anisotropy article fluorescence human in vivo study kinetics Parkinson disease point mutation priority journal protein aggregation protein analysis alpha-Synuclein Amino Acid Sequence Amyloid Anisotropy Fluorescent Dyes Humans Molecular Sequence Data Mutagenesis, Site-Directed Parkinson Disease Protein Folding Protein Structure, Quaternary Pyrenes Spectrometry, Fluorescence Thirunavukkuarasu, S. Jares-Erijman, E.A. Jovin, T.M. Multiparametric Fluorescence Detection of Early Stages in the Amyloid Protein Aggregation of Pyrene-labeled α-Synuclein |
| topic_facet |
fibrillization flourescence anisotropy neurodegenerative oligomers Parkinson's disease alpha synuclein pyrene anisotropy article fluorescence human in vivo study kinetics Parkinson disease point mutation priority journal protein aggregation protein analysis alpha-Synuclein Amino Acid Sequence Amyloid Anisotropy Fluorescent Dyes Humans Molecular Sequence Data Mutagenesis, Site-Directed Parkinson Disease Protein Folding Protein Structure, Quaternary Pyrenes Spectrometry, Fluorescence |
| description |
The aggregation of α-synuclein, a presynaptic protein, has an important role in the etiology of Parkinson's disease. Oligomers or protofibrils adopting the cross-β-sheet structure characteristic of fibrillating amyloid proteins are presumed to be the primary cytotoxic species. Current techniques for monitoring the kinetics of α-synuclein aggregation based on fluorescent dyes such as Thioflavin-T and Congo red detect only the terminal fibrillar species, are discontinuous and notoriously irreproducible. We have devised a new fluorescence aggregation assay that is continuous and provides a large set of fluorescence parameters sensitive to the presence of oligomeric intermediates as well as fibrils. The approach involves tagging functionally neutral Ala-to-Cys variants of α-synuclein with the long-lifetime fluorophore pyrene. Upon induction of aggregation at 37 °C, the entire family of steady-state descriptors of pyrene emission (monomer intensity, solvent polarity ratio (II/IIII), and anisotropy; and excimer intensity) change dramatically, particularly during the early stages in which oligomeric intermediates form and evolve. The pyrene probe senses a progressive decrease in polarity, an increase in molecular mass and close intermolecular association in a manner dependent on position in the sequence and the presence of point mutations. The time-resolved decays (0-160 ns) of intensity and anisotropy exhibited complex, characteristic features. The new assay constitutes a convenient platform for the high-throughput screening of agents useful in the diagnosis and therapy of Parkinson's disease as well as in basic investigations. © 2008 Elsevier Ltd. All rights reserved. |
| format |
JOUR |
| author |
Thirunavukkuarasu, S. Jares-Erijman, E.A. Jovin, T.M. |
| author_facet |
Thirunavukkuarasu, S. Jares-Erijman, E.A. Jovin, T.M. |
| author_sort |
Thirunavukkuarasu, S. |
| title |
Multiparametric Fluorescence Detection of Early Stages in the Amyloid Protein Aggregation of Pyrene-labeled α-Synuclein |
| title_short |
Multiparametric Fluorescence Detection of Early Stages in the Amyloid Protein Aggregation of Pyrene-labeled α-Synuclein |
| title_full |
Multiparametric Fluorescence Detection of Early Stages in the Amyloid Protein Aggregation of Pyrene-labeled α-Synuclein |
| title_fullStr |
Multiparametric Fluorescence Detection of Early Stages in the Amyloid Protein Aggregation of Pyrene-labeled α-Synuclein |
| title_full_unstemmed |
Multiparametric Fluorescence Detection of Early Stages in the Amyloid Protein Aggregation of Pyrene-labeled α-Synuclein |
| title_sort |
multiparametric fluorescence detection of early stages in the amyloid protein aggregation of pyrene-labeled α-synuclein |
| url |
http://hdl.handle.net/20.500.12110/paper_00222836_v378_n5_p1064_Thirunavukkuarasu |
| work_keys_str_mv |
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