Inflammatory changes after cryosurgery-induced necrosis in human melanoma xenografted in nude mice
There is growing evidence that necrosis, instead of apoptosis, could act as a natural adjuvant, which could activate an immune response. In this work we have investigated if induction of tumor necrosis could trigger the affluence of inflammatory cells at the tumor site, and thus induce an immune res...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_0022202X_v116_n5_p664_Gazzaniga |
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todo:paper_0022202X_v116_n5_p664_Gazzaniga2023-10-03T14:28:29Z Inflammatory changes after cryosurgery-induced necrosis in human melanoma xenografted in nude mice Gazzaniga, S. Bravo, A. Goldszmid, S.R. Maschi, F. Martinelli, J. Mordoh, J. Wainstok, R. Cryosurgery Granulocyte-monocyte colony-stimulating factor Inflammatory infiltrate Melanoma liquid nitrogen recombinant granulocyte macrophage colony stimulating factor animal cell animal model antibody response article controlled study cryosurgery cytotoxicity human human cell humoral immunity immune response inflammation inflammatory cell melanoma mouse nonhuman nude mouse priority journal tissue necrosis tumor growth xenograft There is growing evidence that necrosis, instead of apoptosis, could act as a natural adjuvant, which could activate an immune response. In this work we have investigated if induction of tumor necrosis could trigger the affluence of inflammatory cells at the tumor site, and thus induce an immune response. For this purpose, a liquid N2 spray was applied on human melanoma (IIB-MEL-J cell line) xenografted in nude mice and 24 h later some mice received intratumorally a single 500 U dose of recombinant murine granulocyte macrophage-colony-stimulating factor. 77-100% of the tumor mass underwent necrosis. Congestion, edema, and endothelial cell activation were the first noticeable events. A quick infiltrative response of polymorphonuclear leukocytes around the tumor was detected 24 h after liquid N2 application, peaking at day 3. Massive macrophage recruitment was observed since day 3. An early intratumoral infiltration with inflammatory cells was only detected in the group that received recombinant murine granulocyte macrophagecolony-stimulating factor after necrosis induction by liquid N2. Coexisting DEC 205- and F4/80-positive cells increased in number, and their localization was predominantly peritumoral after necrosis. Antibody response was only detected in the groups with tumor-induced necrosis. Our results suggest that cryosurgery-induced necrosis could be a useful model to analyze the interaction among necrosis, inflammation, and the generation of an immune response. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0022202X_v116_n5_p664_Gazzaniga |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cryosurgery Granulocyte-monocyte colony-stimulating factor Inflammatory infiltrate Melanoma liquid nitrogen recombinant granulocyte macrophage colony stimulating factor animal cell animal model antibody response article controlled study cryosurgery cytotoxicity human human cell humoral immunity immune response inflammation inflammatory cell melanoma mouse nonhuman nude mouse priority journal tissue necrosis tumor growth xenograft |
| spellingShingle |
Cryosurgery Granulocyte-monocyte colony-stimulating factor Inflammatory infiltrate Melanoma liquid nitrogen recombinant granulocyte macrophage colony stimulating factor animal cell animal model antibody response article controlled study cryosurgery cytotoxicity human human cell humoral immunity immune response inflammation inflammatory cell melanoma mouse nonhuman nude mouse priority journal tissue necrosis tumor growth xenograft Gazzaniga, S. Bravo, A. Goldszmid, S.R. Maschi, F. Martinelli, J. Mordoh, J. Wainstok, R. Inflammatory changes after cryosurgery-induced necrosis in human melanoma xenografted in nude mice |
| topic_facet |
Cryosurgery Granulocyte-monocyte colony-stimulating factor Inflammatory infiltrate Melanoma liquid nitrogen recombinant granulocyte macrophage colony stimulating factor animal cell animal model antibody response article controlled study cryosurgery cytotoxicity human human cell humoral immunity immune response inflammation inflammatory cell melanoma mouse nonhuman nude mouse priority journal tissue necrosis tumor growth xenograft |
| description |
There is growing evidence that necrosis, instead of apoptosis, could act as a natural adjuvant, which could activate an immune response. In this work we have investigated if induction of tumor necrosis could trigger the affluence of inflammatory cells at the tumor site, and thus induce an immune response. For this purpose, a liquid N2 spray was applied on human melanoma (IIB-MEL-J cell line) xenografted in nude mice and 24 h later some mice received intratumorally a single 500 U dose of recombinant murine granulocyte macrophage-colony-stimulating factor. 77-100% of the tumor mass underwent necrosis. Congestion, edema, and endothelial cell activation were the first noticeable events. A quick infiltrative response of polymorphonuclear leukocytes around the tumor was detected 24 h after liquid N2 application, peaking at day 3. Massive macrophage recruitment was observed since day 3. An early intratumoral infiltration with inflammatory cells was only detected in the group that received recombinant murine granulocyte macrophagecolony-stimulating factor after necrosis induction by liquid N2. Coexisting DEC 205- and F4/80-positive cells increased in number, and their localization was predominantly peritumoral after necrosis. Antibody response was only detected in the groups with tumor-induced necrosis. Our results suggest that cryosurgery-induced necrosis could be a useful model to analyze the interaction among necrosis, inflammation, and the generation of an immune response. |
| format |
JOUR |
| author |
Gazzaniga, S. Bravo, A. Goldszmid, S.R. Maschi, F. Martinelli, J. Mordoh, J. Wainstok, R. |
| author_facet |
Gazzaniga, S. Bravo, A. Goldszmid, S.R. Maschi, F. Martinelli, J. Mordoh, J. Wainstok, R. |
| author_sort |
Gazzaniga, S. |
| title |
Inflammatory changes after cryosurgery-induced necrosis in human melanoma xenografted in nude mice |
| title_short |
Inflammatory changes after cryosurgery-induced necrosis in human melanoma xenografted in nude mice |
| title_full |
Inflammatory changes after cryosurgery-induced necrosis in human melanoma xenografted in nude mice |
| title_fullStr |
Inflammatory changes after cryosurgery-induced necrosis in human melanoma xenografted in nude mice |
| title_full_unstemmed |
Inflammatory changes after cryosurgery-induced necrosis in human melanoma xenografted in nude mice |
| title_sort |
inflammatory changes after cryosurgery-induced necrosis in human melanoma xenografted in nude mice |
| url |
http://hdl.handle.net/20.500.12110/paper_0022202X_v116_n5_p664_Gazzaniga |
| work_keys_str_mv |
AT gazzanigas inflammatorychangesaftercryosurgeryinducednecrosisinhumanmelanomaxenograftedinnudemice AT bravoa inflammatorychangesaftercryosurgeryinducednecrosisinhumanmelanomaxenograftedinnudemice AT goldszmidsr inflammatorychangesaftercryosurgeryinducednecrosisinhumanmelanomaxenograftedinnudemice AT maschif inflammatorychangesaftercryosurgeryinducednecrosisinhumanmelanomaxenograftedinnudemice AT martinellij inflammatorychangesaftercryosurgeryinducednecrosisinhumanmelanomaxenograftedinnudemice AT mordohj inflammatorychangesaftercryosurgeryinducednecrosisinhumanmelanomaxenograftedinnudemice AT wainstokr inflammatorychangesaftercryosurgeryinducednecrosisinhumanmelanomaxenograftedinnudemice |
| _version_ |
1807320835846832128 |