Immune-related gene expression in nurse honey bees (Apis mellifera) exposed to synthetic acaricides
The mite Varroa destructor is an ectoparasite affecting honey bees worldwide. Synthetic acaricides have been among the principal tools available to beekeepers for its control, although several studies have shown its negative effects on honey bee physiology. Recent research suggests that those molecu...
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todo:paper_00221910_v59_n1_p113_Garrido2023-10-03T14:28:21Z Immune-related gene expression in nurse honey bees (Apis mellifera) exposed to synthetic acaricides Garrido, P.M. Antúnez, K. Martín, M. Porrini, M.P. Zunino, P. Eguaras, M.J. Apis mellifera Immune related genes LC50 Synthetic acaricides acaricide insect protein acaricide apiculture cytology ectoparasite enzyme activity gene expression honeybee immunity mite molecular analysis signaling animal article bee drug effect gene expression genetics growth, development and aging immunology synthesis Acaricides Animals Bees Gene Expression Insect Proteins Acari Apis mellifera Apoidea Varroa destructor The mite Varroa destructor is an ectoparasite affecting honey bees worldwide. Synthetic acaricides have been among the principal tools available to beekeepers for its control, although several studies have shown its negative effects on honey bee physiology. Recent research suggests that those molecules strongly impact on immune signaling cascades and cellular immunity. In the present work, LC50 in six-day-old bees were determined for the following acaricides: tau-fluvalinate, flumethrin, amitraz and coumaphos. According to this obtained value, a group of individuals was treated with each acaricide and then processed for qPCR analysis. Transcript levels for genes encoding antimicrobial peptides and immune-related proteins were assessed. Flumethrin increased the expression of hymenoptaecin when comparing treated and control bees. Significant differences were recorded between coumaphos and flumethrin treatments, while the first one reduced the expression of hymenoptaecin and abaecin, the last one up-regulated their expressions. No significant statistically changes were recorded in the expression levels of vitellogenin, lysozyme or glucose dehydrogenase among bees treated with acaricides and control bees. This work constitutes the first report, under laboratory conditions, about induction of immune related genes in response to synthetic miticides. © 2012 Elsevier Ltd. JOUR English info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00221910_v59_n1_p113_Garrido |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
English |
| orig_language_str_mv |
English |
| topic |
Apis mellifera Immune related genes LC50 Synthetic acaricides acaricide insect protein acaricide apiculture cytology ectoparasite enzyme activity gene expression honeybee immunity mite molecular analysis signaling animal article bee drug effect gene expression genetics growth, development and aging immunology synthesis Acaricides Animals Bees Gene Expression Insect Proteins Acari Apis mellifera Apoidea Varroa destructor |
| spellingShingle |
Apis mellifera Immune related genes LC50 Synthetic acaricides acaricide insect protein acaricide apiculture cytology ectoparasite enzyme activity gene expression honeybee immunity mite molecular analysis signaling animal article bee drug effect gene expression genetics growth, development and aging immunology synthesis Acaricides Animals Bees Gene Expression Insect Proteins Acari Apis mellifera Apoidea Varroa destructor Garrido, P.M. Antúnez, K. Martín, M. Porrini, M.P. Zunino, P. Eguaras, M.J. Immune-related gene expression in nurse honey bees (Apis mellifera) exposed to synthetic acaricides |
| topic_facet |
Apis mellifera Immune related genes LC50 Synthetic acaricides acaricide insect protein acaricide apiculture cytology ectoparasite enzyme activity gene expression honeybee immunity mite molecular analysis signaling animal article bee drug effect gene expression genetics growth, development and aging immunology synthesis Acaricides Animals Bees Gene Expression Insect Proteins Acari Apis mellifera Apoidea Varroa destructor |
| description |
The mite Varroa destructor is an ectoparasite affecting honey bees worldwide. Synthetic acaricides have been among the principal tools available to beekeepers for its control, although several studies have shown its negative effects on honey bee physiology. Recent research suggests that those molecules strongly impact on immune signaling cascades and cellular immunity. In the present work, LC50 in six-day-old bees were determined for the following acaricides: tau-fluvalinate, flumethrin, amitraz and coumaphos. According to this obtained value, a group of individuals was treated with each acaricide and then processed for qPCR analysis. Transcript levels for genes encoding antimicrobial peptides and immune-related proteins were assessed. Flumethrin increased the expression of hymenoptaecin when comparing treated and control bees. Significant differences were recorded between coumaphos and flumethrin treatments, while the first one reduced the expression of hymenoptaecin and abaecin, the last one up-regulated their expressions. No significant statistically changes were recorded in the expression levels of vitellogenin, lysozyme or glucose dehydrogenase among bees treated with acaricides and control bees. This work constitutes the first report, under laboratory conditions, about induction of immune related genes in response to synthetic miticides. © 2012 Elsevier Ltd. |
| format |
JOUR |
| author |
Garrido, P.M. Antúnez, K. Martín, M. Porrini, M.P. Zunino, P. Eguaras, M.J. |
| author_facet |
Garrido, P.M. Antúnez, K. Martín, M. Porrini, M.P. Zunino, P. Eguaras, M.J. |
| author_sort |
Garrido, P.M. |
| title |
Immune-related gene expression in nurse honey bees (Apis mellifera) exposed to synthetic acaricides |
| title_short |
Immune-related gene expression in nurse honey bees (Apis mellifera) exposed to synthetic acaricides |
| title_full |
Immune-related gene expression in nurse honey bees (Apis mellifera) exposed to synthetic acaricides |
| title_fullStr |
Immune-related gene expression in nurse honey bees (Apis mellifera) exposed to synthetic acaricides |
| title_full_unstemmed |
Immune-related gene expression in nurse honey bees (Apis mellifera) exposed to synthetic acaricides |
| title_sort |
immune-related gene expression in nurse honey bees (apis mellifera) exposed to synthetic acaricides |
| url |
http://hdl.handle.net/20.500.12110/paper_00221910_v59_n1_p113_Garrido |
| work_keys_str_mv |
AT garridopm immunerelatedgeneexpressioninnursehoneybeesapismelliferaexposedtosyntheticacaricides AT antunezk immunerelatedgeneexpressioninnursehoneybeesapismelliferaexposedtosyntheticacaricides AT martinm immunerelatedgeneexpressioninnursehoneybeesapismelliferaexposedtosyntheticacaricides AT porrinimp immunerelatedgeneexpressioninnursehoneybeesapismelliferaexposedtosyntheticacaricides AT zuninop immunerelatedgeneexpressioninnursehoneybeesapismelliferaexposedtosyntheticacaricides AT eguarasmj immunerelatedgeneexpressioninnursehoneybeesapismelliferaexposedtosyntheticacaricides |
| _version_ |
1782024971391860736 |