Raf/MEK/ERK pathway activation is required for Junín virus replication

In the present work we investigated the importance of the Raf/MEK/ERK signalling pathway in the multiplication of the arenavirus Junín (JUNV) in monkey and human cell cultures. We established that JUNV induces a biphasic activation of ERK and we proved that a specific inhibitor of the ERK pathway, U...

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Autores principales:	Rodríguez, M.E., Brunetti, J.E., Wachsman, M.B., Scolaro, L.A., Castilla, V.
Formato:	JOUR
Materias:	1,4 diamino 1,4 bis(2 aminophenylthio) 2,3 dicyanobutadiene mitogen activated protein kinase phorbol 13 acetate 12 myristate small interfering RNA animal cell Arenavirus arenavirus infection Argentine hemorrhagic fever article cell culture cell strain U937 controlled study enzyme activation enzyme phosphorylation human human cell Junin virus nonhuman priority journal signal transduction Vero cell virus replication Animals Cell Line Extracellular Signal-Regulated MAP Kinases Gene Knockdown Techniques Haplorhini Host-Pathogen Interactions Humans MAP Kinase Signaling System Virus Replication
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00221317_v95_nPART4_p799_Rodriguez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_00221317_v95_nPART4_p799_Rodriguez2023-10-03T14:27:28Z Raf/MEK/ERK pathway activation is required for Junín virus replication Rodríguez, M.E. Brunetti, J.E. Wachsman, M.B. Scolaro, L.A. Castilla, V. 1,4 diamino 1,4 bis(2 aminophenylthio) 2,3 dicyanobutadiene mitogen activated protein kinase phorbol 13 acetate 12 myristate small interfering RNA animal cell Arenavirus arenavirus infection Argentine hemorrhagic fever article cell culture cell strain U937 controlled study enzyme activation enzyme phosphorylation human human cell Junin virus nonhuman priority journal signal transduction Vero cell virus replication Animals Cell Line Extracellular Signal-Regulated MAP Kinases Gene Knockdown Techniques Haplorhini Host-Pathogen Interactions Humans Junin virus MAP Kinase Signaling System Virus Replication In the present work we investigated the importance of the Raf/MEK/ERK signalling pathway in the multiplication of the arenavirus Junín (JUNV) in monkey and human cell cultures. We established that JUNV induces a biphasic activation of ERK and we proved that a specific inhibitor of the ERK pathway, U0126, impairs viral replication. Furthermore, U0126 exerted inhibitory action against the arenaviruses Tacaribe and Pichinde. Moreover, treatment with known ERK activators such as phorbol 12-myristate 13-acetate and serum increased viral yields whereas ERK silencing by small interfering RNAs caused the inhibition of viral multiplication. Therefore, activation of the Raf/MEK/ ERK signalling pathway is required to ensure efficient JUNV replication and may constitute a host target for the development of novel effective therapeutic strategies to deal with arenavirus infections. © 2014 SGM. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00221317_v95_nPART4_p799_Rodriguez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	1,4 diamino 1,4 bis(2 aminophenylthio) 2,3 dicyanobutadiene mitogen activated protein kinase phorbol 13 acetate 12 myristate small interfering RNA animal cell Arenavirus arenavirus infection Argentine hemorrhagic fever article cell culture cell strain U937 controlled study enzyme activation enzyme phosphorylation human human cell Junin virus nonhuman priority journal signal transduction Vero cell virus replication Animals Cell Line Extracellular Signal-Regulated MAP Kinases Gene Knockdown Techniques Haplorhini Host-Pathogen Interactions Humans Junin virus MAP Kinase Signaling System Virus Replication
spellingShingle	1,4 diamino 1,4 bis(2 aminophenylthio) 2,3 dicyanobutadiene mitogen activated protein kinase phorbol 13 acetate 12 myristate small interfering RNA animal cell Arenavirus arenavirus infection Argentine hemorrhagic fever article cell culture cell strain U937 controlled study enzyme activation enzyme phosphorylation human human cell Junin virus nonhuman priority journal signal transduction Vero cell virus replication Animals Cell Line Extracellular Signal-Regulated MAP Kinases Gene Knockdown Techniques Haplorhini Host-Pathogen Interactions Humans Junin virus MAP Kinase Signaling System Virus Replication Rodríguez, M.E. Brunetti, J.E. Wachsman, M.B. Scolaro, L.A. Castilla, V. Raf/MEK/ERK pathway activation is required for Junín virus replication
topic_facet	1,4 diamino 1,4 bis(2 aminophenylthio) 2,3 dicyanobutadiene mitogen activated protein kinase phorbol 13 acetate 12 myristate small interfering RNA animal cell Arenavirus arenavirus infection Argentine hemorrhagic fever article cell culture cell strain U937 controlled study enzyme activation enzyme phosphorylation human human cell Junin virus nonhuman priority journal signal transduction Vero cell virus replication Animals Cell Line Extracellular Signal-Regulated MAP Kinases Gene Knockdown Techniques Haplorhini Host-Pathogen Interactions Humans Junin virus MAP Kinase Signaling System Virus Replication
description	In the present work we investigated the importance of the Raf/MEK/ERK signalling pathway in the multiplication of the arenavirus Junín (JUNV) in monkey and human cell cultures. We established that JUNV induces a biphasic activation of ERK and we proved that a specific inhibitor of the ERK pathway, U0126, impairs viral replication. Furthermore, U0126 exerted inhibitory action against the arenaviruses Tacaribe and Pichinde. Moreover, treatment with known ERK activators such as phorbol 12-myristate 13-acetate and serum increased viral yields whereas ERK silencing by small interfering RNAs caused the inhibition of viral multiplication. Therefore, activation of the Raf/MEK/ ERK signalling pathway is required to ensure efficient JUNV replication and may constitute a host target for the development of novel effective therapeutic strategies to deal with arenavirus infections. © 2014 SGM.
format	JOUR
author	Rodríguez, M.E. Brunetti, J.E. Wachsman, M.B. Scolaro, L.A. Castilla, V.
author_facet	Rodríguez, M.E. Brunetti, J.E. Wachsman, M.B. Scolaro, L.A. Castilla, V.
author_sort	Rodríguez, M.E.
title	Raf/MEK/ERK pathway activation is required for Junín virus replication
title_short	Raf/MEK/ERK pathway activation is required for Junín virus replication
title_full	Raf/MEK/ERK pathway activation is required for Junín virus replication
title_fullStr	Raf/MEK/ERK pathway activation is required for Junín virus replication
title_full_unstemmed	Raf/MEK/ERK pathway activation is required for Junín virus replication
title_sort	raf/mek/erk pathway activation is required for junín virus replication
url	http://hdl.handle.net/20.500.12110/paper_00221317_v95_nPART4_p799_Rodriguez
work_keys_str_mv	AT rodriguezme rafmekerkpathwayactivationisrequiredforjuninvirusreplication AT brunettije rafmekerkpathwayactivationisrequiredforjuninvirusreplication AT wachsmanmb rafmekerkpathwayactivationisrequiredforjuninvirusreplication AT scolarola rafmekerkpathwayactivationisrequiredforjuninvirusreplication AT castillav rafmekerkpathwayactivationisrequiredforjuninvirusreplication
_version_	1782024107895816192

Raf/MEK/ERK pathway activation is required for Junín virus replication

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