Pituitary adenylate cyclase-activating polypeptide, interleukin-6 and glucocorticoids regulate the release of vascular endothelial growth factor in pituitary folliculostellate cells

There is increasing evidence that hormones play an important role in the control of endothelial cell function and growth by regulating the production of vascular endothelial growth factor (VEGF). VEGF regulates vascular permeability and represents the most powerful growth factor for endothelial cell...

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Autores principales: Gloddek, J., Pagotto, U., Paez Pereda, M., Arzt, E., Stalla, G.K., Renner, U.
Formato: JOUR
Materias:
dexamethasone
glucocorticoid
hypophysis adenylate cyclase activating polypeptide
interleukin 6
mifepristone
vasculotropin
angiogenesis
animal cell
article
blood vessel permeability
cell function
cell growth
endothelium cell
hormonal regulation
hormone blood level
hormone release
inhibition kinetics
nonhuman
priority journal
protein expression
rat
receptor upregulation
regulatory mechanism
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00220795_v160_n3_p483_Gloddek
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00220795_v160_n3_p483_Gloddek
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spelling todo:paper_00220795_v160_n3_p483_Gloddek2023-10-03T14:25:51Z Pituitary adenylate cyclase-activating polypeptide, interleukin-6 and glucocorticoids regulate the release of vascular endothelial growth factor in pituitary folliculostellate cells Gloddek, J. Pagotto, U. Paez Pereda, M. Arzt, E. Stalla, G.K. Renner, U. dexamethasone glucocorticoid hypophysis adenylate cyclase activating polypeptide interleukin 6 mifepristone vasculotropin angiogenesis animal cell article blood vessel permeability cell function cell growth endothelium cell hormonal regulation hormone blood level hormone release inhibition kinetics nonhuman priority journal protein expression rat receptor upregulation regulatory mechanism There is increasing evidence that hormones play an important role in the control of endothelial cell function and growth by regulating the production of vascular endothelial growth factor (VEGF). VEGF regulates vascular permeability and represents the most powerful growth factor for endothelial cells. In the normal anterior pituitary, VEGF has been detected only in folliculostellate (FS) cells. In the present study, the regulation of the release of VEGF from FS-like mouse TtT/GF cells, and from FS cells of rat pituitary monolayer cell cultures was investigated using a specific VEGF ELISA. Basal release of VEGF was demonstrated in cultures of both TtT/GF cells and rat pituitary cells. Interestingly, the VEGF secretion was stimulated by both forms of pituitary adenylate cyclase-activating polypeptide (PACAP-38 and PACAP-27), indicating that this hypothalamic peptide regulates endothelial cell function and growth within the pituitary. VEGF secretion was also stimulated by interleukin-6 (IL-6) whereas basal, IL- 6- and PACAP-stimulated secretion was inhibited by the synthetic glucocorticoid dexamethasone. The inhibitory action of dexamethasone was reversed by the glucocorticoid receptor antagonist RU486, suggesting that in FS cells functional glucocorticoid receptors mediate the inhibitory action of glucocorticoids on the VEGF secretion. The endocrine and auto-/paracrine control of VEGF production in pituitary FS cells by PACAP, IL-6 and glucocorticoids may play an important role both in angiogenesis and vascular permeability regulation within the pituitary under physiological and pathophysiological conditions. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00220795_v160_n3_p483_Gloddek
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic dexamethasone
glucocorticoid
hypophysis adenylate cyclase activating polypeptide
interleukin 6
mifepristone
vasculotropin
angiogenesis
animal cell
article
blood vessel permeability
cell function
cell growth
endothelium cell
hormonal regulation
hormone blood level
hormone release
inhibition kinetics
nonhuman
priority journal
protein expression
rat
receptor upregulation
regulatory mechanism
spellingShingle dexamethasone
glucocorticoid
hypophysis adenylate cyclase activating polypeptide
interleukin 6
mifepristone
vasculotropin
angiogenesis
animal cell
article
blood vessel permeability
cell function
cell growth
endothelium cell
hormonal regulation
hormone blood level
hormone release
inhibition kinetics
nonhuman
priority journal
protein expression
rat
receptor upregulation
regulatory mechanism
Gloddek, J.
Pagotto, U.
Paez Pereda, M.
Arzt, E.
Stalla, G.K.
Renner, U.
Pituitary adenylate cyclase-activating polypeptide, interleukin-6 and glucocorticoids regulate the release of vascular endothelial growth factor in pituitary folliculostellate cells
topic_facet dexamethasone
glucocorticoid
hypophysis adenylate cyclase activating polypeptide
interleukin 6
mifepristone
vasculotropin
angiogenesis
animal cell
article
blood vessel permeability
cell function
cell growth
endothelium cell
hormonal regulation
hormone blood level
hormone release
inhibition kinetics
nonhuman
priority journal
protein expression
rat
receptor upregulation
regulatory mechanism
description There is increasing evidence that hormones play an important role in the control of endothelial cell function and growth by regulating the production of vascular endothelial growth factor (VEGF). VEGF regulates vascular permeability and represents the most powerful growth factor for endothelial cells. In the normal anterior pituitary, VEGF has been detected only in folliculostellate (FS) cells. In the present study, the regulation of the release of VEGF from FS-like mouse TtT/GF cells, and from FS cells of rat pituitary monolayer cell cultures was investigated using a specific VEGF ELISA. Basal release of VEGF was demonstrated in cultures of both TtT/GF cells and rat pituitary cells. Interestingly, the VEGF secretion was stimulated by both forms of pituitary adenylate cyclase-activating polypeptide (PACAP-38 and PACAP-27), indicating that this hypothalamic peptide regulates endothelial cell function and growth within the pituitary. VEGF secretion was also stimulated by interleukin-6 (IL-6) whereas basal, IL- 6- and PACAP-stimulated secretion was inhibited by the synthetic glucocorticoid dexamethasone. The inhibitory action of dexamethasone was reversed by the glucocorticoid receptor antagonist RU486, suggesting that in FS cells functional glucocorticoid receptors mediate the inhibitory action of glucocorticoids on the VEGF secretion. The endocrine and auto-/paracrine control of VEGF production in pituitary FS cells by PACAP, IL-6 and glucocorticoids may play an important role both in angiogenesis and vascular permeability regulation within the pituitary under physiological and pathophysiological conditions.
format JOUR
author Gloddek, J.
Pagotto, U.
Paez Pereda, M.
Arzt, E.
Stalla, G.K.
Renner, U.
author_facet Gloddek, J.
Pagotto, U.
Paez Pereda, M.
Arzt, E.
Stalla, G.K.
Renner, U.
author_sort Gloddek, J.
title Pituitary adenylate cyclase-activating polypeptide, interleukin-6 and glucocorticoids regulate the release of vascular endothelial growth factor in pituitary folliculostellate cells
title_short Pituitary adenylate cyclase-activating polypeptide, interleukin-6 and glucocorticoids regulate the release of vascular endothelial growth factor in pituitary folliculostellate cells
title_full Pituitary adenylate cyclase-activating polypeptide, interleukin-6 and glucocorticoids regulate the release of vascular endothelial growth factor in pituitary folliculostellate cells
title_fullStr Pituitary adenylate cyclase-activating polypeptide, interleukin-6 and glucocorticoids regulate the release of vascular endothelial growth factor in pituitary folliculostellate cells
title_full_unstemmed Pituitary adenylate cyclase-activating polypeptide, interleukin-6 and glucocorticoids regulate the release of vascular endothelial growth factor in pituitary folliculostellate cells
title_sort pituitary adenylate cyclase-activating polypeptide, interleukin-6 and glucocorticoids regulate the release of vascular endothelial growth factor in pituitary folliculostellate cells
url http://hdl.handle.net/20.500.12110/paper_00220795_v160_n3_p483_Gloddek
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AT pagottou pituitaryadenylatecyclaseactivatingpolypeptideinterleukin6andglucocorticoidsregulatethereleaseofvascularendothelialgrowthfactorinpituitaryfolliculostellatecells
AT paezperedam pituitaryadenylatecyclaseactivatingpolypeptideinterleukin6andglucocorticoidsregulatethereleaseofvascularendothelialgrowthfactorinpituitaryfolliculostellatecells
AT arzte pituitaryadenylatecyclaseactivatingpolypeptideinterleukin6andglucocorticoidsregulatethereleaseofvascularendothelialgrowthfactorinpituitaryfolliculostellatecells
AT stallagk pituitaryadenylatecyclaseactivatingpolypeptideinterleukin6andglucocorticoidsregulatethereleaseofvascularendothelialgrowthfactorinpituitaryfolliculostellatecells
AT renneru pituitaryadenylatecyclaseactivatingpolypeptideinterleukin6andglucocorticoidsregulatethereleaseofvascularendothelialgrowthfactorinpituitaryfolliculostellatecells
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