Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 2. Bienzymatic configuration
Four configurations of an enzyme electrode to assess lactate concentration in dairy products are studied. In these bienzymatic multilayer systems, the inner layer was constructed by crosslinking peroxidase with poly(allylamine) containing an osmium complex (OsPAA), and an outer layer containing lact...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00220728_v511_n1-2_p8_Corton |
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todo:paper_00220728_v511_n1-2_p8_Corton2023-10-03T14:25:49Z Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 2. Bienzymatic configuration Cortón, E. Battaglini, F. Biosensors Chronoamperometry Cyclic voltammetry Flow injection analysis Redox enzymes Cathodes Crosslinking Cyclic voltammetry Electrochemical electrodes Enzyme immobilization Ionic strength Osmium compounds Proteins Redox reactions Cathodic currents Biosensors Four configurations of an enzyme electrode to assess lactate concentration in dairy products are studied. In these bienzymatic multilayer systems, the inner layer was constructed by crosslinking peroxidase with poly(allylamine) containing an osmium complex (OsPAA), and an outer layer containing lactate oxidase (LOx) immobilized in polymeric matrices. When the hydrogen peroxide formed by the LOx layer reaches the inner layer, the electronic flow between the wired peroxidase and the electrode surface produces a cathodic current, proportional to lactate concentration. Parameters such as the effect of ionic strength, stability when the electrode is used as detector in a FIA system and reproducibility were examined. This arrangement allows working in undiluted milk samples. © 2001 Elsevier Science B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00220728_v511_n1-2_p8_Corton |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Biosensors Chronoamperometry Cyclic voltammetry Flow injection analysis Redox enzymes Cathodes Crosslinking Cyclic voltammetry Electrochemical electrodes Enzyme immobilization Ionic strength Osmium compounds Proteins Redox reactions Cathodic currents Biosensors |
| spellingShingle |
Biosensors Chronoamperometry Cyclic voltammetry Flow injection analysis Redox enzymes Cathodes Crosslinking Cyclic voltammetry Electrochemical electrodes Enzyme immobilization Ionic strength Osmium compounds Proteins Redox reactions Cathodic currents Biosensors Cortón, E. Battaglini, F. Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 2. Bienzymatic configuration |
| topic_facet |
Biosensors Chronoamperometry Cyclic voltammetry Flow injection analysis Redox enzymes Cathodes Crosslinking Cyclic voltammetry Electrochemical electrodes Enzyme immobilization Ionic strength Osmium compounds Proteins Redox reactions Cathodic currents Biosensors |
| description |
Four configurations of an enzyme electrode to assess lactate concentration in dairy products are studied. In these bienzymatic multilayer systems, the inner layer was constructed by crosslinking peroxidase with poly(allylamine) containing an osmium complex (OsPAA), and an outer layer containing lactate oxidase (LOx) immobilized in polymeric matrices. When the hydrogen peroxide formed by the LOx layer reaches the inner layer, the electronic flow between the wired peroxidase and the electrode surface produces a cathodic current, proportional to lactate concentration. Parameters such as the effect of ionic strength, stability when the electrode is used as detector in a FIA system and reproducibility were examined. This arrangement allows working in undiluted milk samples. © 2001 Elsevier Science B.V. |
| format |
JOUR |
| author |
Cortón, E. Battaglini, F. |
| author_facet |
Cortón, E. Battaglini, F. |
| author_sort |
Cortón, E. |
| title |
Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 2. Bienzymatic configuration |
| title_short |
Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 2. Bienzymatic configuration |
| title_full |
Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 2. Bienzymatic configuration |
| title_fullStr |
Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 2. Bienzymatic configuration |
| title_full_unstemmed |
Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 2. Bienzymatic configuration |
| title_sort |
effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: part 2. bienzymatic configuration |
| url |
http://hdl.handle.net/20.500.12110/paper_00220728_v511_n1-2_p8_Corton |
| work_keys_str_mv |
AT cortone effectofmilkproteinsonthebehaviorofabiosensorbasedonpolyallylaminecontaininganosmiumcomplexwiredtoredoxenzymespart2bienzymaticconfiguration AT battaglinif effectofmilkproteinsonthebehaviorofabiosensorbasedonpolyallylaminecontaininganosmiumcomplexwiredtoredoxenzymespart2bienzymaticconfiguration |
| _version_ |
1807318666979573760 |