Phloxine B Effect on Immature Stages of the Mediterranean Fruit Fly, Ceratitis capitata (Diptera: Tephritidae) (Wiedemann)
A laboratory bioassay was developed to determine both the chemical toxicity and the phototoxicity of the xanthene dye, phloxine B (D&C Red N°28), to the immature stages of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann). An additional goal was to find out which main tissues are a...
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todo:paper_00220493_v96_n3_p662_Berni2023-10-03T14:25:42Z Phloxine B Effect on Immature Stages of the Mediterranean Fruit Fly, Ceratitis capitata (Diptera: Tephritidae) (Wiedemann) Berni, J. Rabossi, A. Quesada-Allué, L.A. Ceratitis capitata Mediterranean fruit fly Metamorphosis Phloxine B Photoinsecticides eosine blue animal article darkness drug effect growth, development and aging larva LD 50 life cycle light Mediterranean fruit fly motor activity physiology Animals Ceratitis capitata Darkness Eosine I Bluish Larva Lethal Dose 50 Life Cycle Stages Light Motor Activity Animalia Capitata Ceratitis Ceratitis capitata Diptera Drosophila melanogaster Hexapoda Invertebrata Tephritidae A laboratory bioassay was developed to determine both the chemical toxicity and the phototoxicity of the xanthene dye, phloxine B (D&C Red N°28), to the immature stages of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann). An additional goal was to find out which main tissues are affected first. A low, but significant, level of toxicity was observed when the insects were maintained in the dark: at the point of adult ecdysis, the LC 50 was 11.03 mM. As expected, after 8-h exposure of late larva III to light, a high level of mortality was produced (LC50 at ecdysis: 0.45 mM) as a dose-dependent function of dye concentration. At sublethal concentrations of the dye, the surviving insects showed a number of physiological abnormalities. Phloxine B appeared to mainly affect the larval longitudinal muscles as well as the abdominal muscles of ecdysing adults, giving rise to abnormal puparia and failed adult ecdysis, respectively. Moreover, a significant phloxine B-dependent delay in the jumping of surviving larvae for dispersal was documented. This could be attributed to a delay in attaining a threshold weight for jumping and/or to abnormalities in neuromuscular coordination, thus reinforcing the idea of pleiotropic effects of the dye. Fil:Berni, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rabossi, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Quesada-Allué, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00220493_v96_n3_p662_Berni |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Ceratitis capitata Mediterranean fruit fly Metamorphosis Phloxine B Photoinsecticides eosine blue animal article darkness drug effect growth, development and aging larva LD 50 life cycle light Mediterranean fruit fly motor activity physiology Animals Ceratitis capitata Darkness Eosine I Bluish Larva Lethal Dose 50 Life Cycle Stages Light Motor Activity Animalia Capitata Ceratitis Ceratitis capitata Diptera Drosophila melanogaster Hexapoda Invertebrata Tephritidae |
| spellingShingle |
Ceratitis capitata Mediterranean fruit fly Metamorphosis Phloxine B Photoinsecticides eosine blue animal article darkness drug effect growth, development and aging larva LD 50 life cycle light Mediterranean fruit fly motor activity physiology Animals Ceratitis capitata Darkness Eosine I Bluish Larva Lethal Dose 50 Life Cycle Stages Light Motor Activity Animalia Capitata Ceratitis Ceratitis capitata Diptera Drosophila melanogaster Hexapoda Invertebrata Tephritidae Berni, J. Rabossi, A. Quesada-Allué, L.A. Phloxine B Effect on Immature Stages of the Mediterranean Fruit Fly, Ceratitis capitata (Diptera: Tephritidae) (Wiedemann) |
| topic_facet |
Ceratitis capitata Mediterranean fruit fly Metamorphosis Phloxine B Photoinsecticides eosine blue animal article darkness drug effect growth, development and aging larva LD 50 life cycle light Mediterranean fruit fly motor activity physiology Animals Ceratitis capitata Darkness Eosine I Bluish Larva Lethal Dose 50 Life Cycle Stages Light Motor Activity Animalia Capitata Ceratitis Ceratitis capitata Diptera Drosophila melanogaster Hexapoda Invertebrata Tephritidae |
| description |
A laboratory bioassay was developed to determine both the chemical toxicity and the phototoxicity of the xanthene dye, phloxine B (D&C Red N°28), to the immature stages of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann). An additional goal was to find out which main tissues are affected first. A low, but significant, level of toxicity was observed when the insects were maintained in the dark: at the point of adult ecdysis, the LC 50 was 11.03 mM. As expected, after 8-h exposure of late larva III to light, a high level of mortality was produced (LC50 at ecdysis: 0.45 mM) as a dose-dependent function of dye concentration. At sublethal concentrations of the dye, the surviving insects showed a number of physiological abnormalities. Phloxine B appeared to mainly affect the larval longitudinal muscles as well as the abdominal muscles of ecdysing adults, giving rise to abnormal puparia and failed adult ecdysis, respectively. Moreover, a significant phloxine B-dependent delay in the jumping of surviving larvae for dispersal was documented. This could be attributed to a delay in attaining a threshold weight for jumping and/or to abnormalities in neuromuscular coordination, thus reinforcing the idea of pleiotropic effects of the dye. |
| format |
JOUR |
| author |
Berni, J. Rabossi, A. Quesada-Allué, L.A. |
| author_facet |
Berni, J. Rabossi, A. Quesada-Allué, L.A. |
| author_sort |
Berni, J. |
| title |
Phloxine B Effect on Immature Stages of the Mediterranean Fruit Fly, Ceratitis capitata (Diptera: Tephritidae) (Wiedemann) |
| title_short |
Phloxine B Effect on Immature Stages of the Mediterranean Fruit Fly, Ceratitis capitata (Diptera: Tephritidae) (Wiedemann) |
| title_full |
Phloxine B Effect on Immature Stages of the Mediterranean Fruit Fly, Ceratitis capitata (Diptera: Tephritidae) (Wiedemann) |
| title_fullStr |
Phloxine B Effect on Immature Stages of the Mediterranean Fruit Fly, Ceratitis capitata (Diptera: Tephritidae) (Wiedemann) |
| title_full_unstemmed |
Phloxine B Effect on Immature Stages of the Mediterranean Fruit Fly, Ceratitis capitata (Diptera: Tephritidae) (Wiedemann) |
| title_sort |
phloxine b effect on immature stages of the mediterranean fruit fly, ceratitis capitata (diptera: tephritidae) (wiedemann) |
| url |
http://hdl.handle.net/20.500.12110/paper_00220493_v96_n3_p662_Berni |
| work_keys_str_mv |
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1807317399881383936 |