Antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria

Bovine mastitis is one of the most economically deleterious diseases affecting dairy herds and results from an infection of the udder by pathogenic microorganisms such as Staphylococcus aureus, Streptococcus uberis, and Escherichia coli. The mammary gland is capable of preventing and combating bacte...

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Autores principales:	Chaneton, L., Pérez Sáez, J.M., Bussmann, L.E.
Formato:	JOUR
Materias:	β-lactoglobulin Antimicrobial Mastitis-causing bacteria antiinfective agent lactoglobulin animal animal disease article bacterial count bovine mastitis cattle classification drug effect Escherichia coli female genetics growth, development and aging microbiology Staphylococcus aureus Streptococcus Animals Anti-Infective Agents Cattle Colony Count, Microbial Female Lactoglobulins Mastitis, Bovine Bacteria (microorganisms) Bovinae Lagomorpha Mammalia Rodentia Streptococcus uberis
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00220302_v94_n1_p138_Chaneton
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00220302_v94_n1_p138_Chaneton
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spelling	todo:paper_00220302_v94_n1_p138_Chaneton2023-10-03T14:25:28Z Antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria Chaneton, L. Pérez Sáez, J.M. Bussmann, L.E. β-lactoglobulin Antimicrobial Mastitis-causing bacteria antiinfective agent lactoglobulin animal animal disease article bacterial count bovine mastitis cattle classification drug effect Escherichia coli female genetics growth, development and aging microbiology Staphylococcus aureus Streptococcus Animals Anti-Infective Agents Cattle Colony Count, Microbial Escherichia coli Female Lactoglobulins Mastitis, Bovine Staphylococcus aureus Streptococcus Bacteria (microorganisms) Bovinae Escherichia coli Lagomorpha Mammalia Rodentia Staphylococcus aureus Streptococcus uberis Bovine mastitis is one of the most economically deleterious diseases affecting dairy herds and results from an infection of the udder by pathogenic microorganisms such as Staphylococcus aureus, Streptococcus uberis, and Escherichia coli. The mammary gland is capable of preventing and combating bacterial infection by means of a complex network of innate and adaptive immune mechanisms. Lactoferrin is an 86-kDa protein with antibacterial activity that plays a role in the mammary gland's defense against infection. β-Lactoglobulin (β-LG) is an 18-kDa protein that is present in most mammals but is notably absent in humans, rodents, and lagomorphs. Different genetic variants of this protein exist, with β-LG A and β-LG B being the most common. In spite of being well studied, the biological function of β-LG is not thoroughly understood, and most noticeably, no reports exist on the effects of the native protein on bacterial growth. Hence, the objective of this study was to assess the potential antibacterial activity of β-LG against mastitis agents. To do this, we purified β-LG from normal bovine milk using a mild, nondenaturing method and performed in vitro growth inhibition assays with Staph. aureus, E. coli, and Strep. uberis. β-Lactoglobulin inhibited the growth of Staph. aureus and Strep. uberis but had no effect on E. coli. The antimicrobial activity against Staph. aureus and Strep. uberis was concentration dependent and was elicited by the intact protein because Tricine-sodium dodecyl sulfate-PAGE and analytical gel filtration chromatography did not reveal the presence of short degradation peptides. Analysis of the genetic variants of β-LG showed that β-LG A has higher inhibitory activity against Staph. aureus and Strep. uberis than β-LG B. Coincubation of β-LG and lactoferrin resulted in an augmented antibacterial activity against Staph. aureus, suggesting an additive effect of the proteins. This result, along with the proteins' complementary spectrum of action, suggests that β-LG and lactoferrin may complement each other in the mammary gland's defenses against bacterial infection. © 2011 American Dairy Science Association. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00220302_v94_n1_p138_Chaneton
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	β-lactoglobulin Antimicrobial Mastitis-causing bacteria antiinfective agent lactoglobulin animal animal disease article bacterial count bovine mastitis cattle classification drug effect Escherichia coli female genetics growth, development and aging microbiology Staphylococcus aureus Streptococcus Animals Anti-Infective Agents Cattle Colony Count, Microbial Escherichia coli Female Lactoglobulins Mastitis, Bovine Staphylococcus aureus Streptococcus Bacteria (microorganisms) Bovinae Escherichia coli Lagomorpha Mammalia Rodentia Staphylococcus aureus Streptococcus uberis
spellingShingle	β-lactoglobulin Antimicrobial Mastitis-causing bacteria antiinfective agent lactoglobulin animal animal disease article bacterial count bovine mastitis cattle classification drug effect Escherichia coli female genetics growth, development and aging microbiology Staphylococcus aureus Streptococcus Animals Anti-Infective Agents Cattle Colony Count, Microbial Escherichia coli Female Lactoglobulins Mastitis, Bovine Staphylococcus aureus Streptococcus Bacteria (microorganisms) Bovinae Escherichia coli Lagomorpha Mammalia Rodentia Staphylococcus aureus Streptococcus uberis Chaneton, L. Pérez Sáez, J.M. Bussmann, L.E. Antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria
topic_facet	β-lactoglobulin Antimicrobial Mastitis-causing bacteria antiinfective agent lactoglobulin animal animal disease article bacterial count bovine mastitis cattle classification drug effect Escherichia coli female genetics growth, development and aging microbiology Staphylococcus aureus Streptococcus Animals Anti-Infective Agents Cattle Colony Count, Microbial Escherichia coli Female Lactoglobulins Mastitis, Bovine Staphylococcus aureus Streptococcus Bacteria (microorganisms) Bovinae Escherichia coli Lagomorpha Mammalia Rodentia Staphylococcus aureus Streptococcus uberis
description	Bovine mastitis is one of the most economically deleterious diseases affecting dairy herds and results from an infection of the udder by pathogenic microorganisms such as Staphylococcus aureus, Streptococcus uberis, and Escherichia coli. The mammary gland is capable of preventing and combating bacterial infection by means of a complex network of innate and adaptive immune mechanisms. Lactoferrin is an 86-kDa protein with antibacterial activity that plays a role in the mammary gland's defense against infection. β-Lactoglobulin (β-LG) is an 18-kDa protein that is present in most mammals but is notably absent in humans, rodents, and lagomorphs. Different genetic variants of this protein exist, with β-LG A and β-LG B being the most common. In spite of being well studied, the biological function of β-LG is not thoroughly understood, and most noticeably, no reports exist on the effects of the native protein on bacterial growth. Hence, the objective of this study was to assess the potential antibacterial activity of β-LG against mastitis agents. To do this, we purified β-LG from normal bovine milk using a mild, nondenaturing method and performed in vitro growth inhibition assays with Staph. aureus, E. coli, and Strep. uberis. β-Lactoglobulin inhibited the growth of Staph. aureus and Strep. uberis but had no effect on E. coli. The antimicrobial activity against Staph. aureus and Strep. uberis was concentration dependent and was elicited by the intact protein because Tricine-sodium dodecyl sulfate-PAGE and analytical gel filtration chromatography did not reveal the presence of short degradation peptides. Analysis of the genetic variants of β-LG showed that β-LG A has higher inhibitory activity against Staph. aureus and Strep. uberis than β-LG B. Coincubation of β-LG and lactoferrin resulted in an augmented antibacterial activity against Staph. aureus, suggesting an additive effect of the proteins. This result, along with the proteins' complementary spectrum of action, suggests that β-LG and lactoferrin may complement each other in the mammary gland's defenses against bacterial infection. © 2011 American Dairy Science Association.
format	JOUR
author	Chaneton, L. Pérez Sáez, J.M. Bussmann, L.E.
author_facet	Chaneton, L. Pérez Sáez, J.M. Bussmann, L.E.
author_sort	Chaneton, L.
title	Antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria
title_short	Antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria
title_full	Antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria
title_fullStr	Antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria
title_full_unstemmed	Antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria
title_sort	antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria
url	http://hdl.handle.net/20.500.12110/paper_00220302_v94_n1_p138_Chaneton
work_keys_str_mv	AT chanetonl antimicrobialactivityofbovineblactoglobulinagainstmastitiscausingbacteria AT perezsaezjm antimicrobialactivityofbovineblactoglobulinagainstmastitiscausingbacteria AT bussmannle antimicrobialactivityofbovineblactoglobulinagainstmastitiscausingbacteria
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Antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria

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