Estimation of molecular weights of proteins by bio-gel P gel filtration

Correlation between elution volumes, Ve, and molecular weight was investigated by Bio-Gel filtration of proteins of known molecular weight on Bio-Gel P-100, P-200 and P-300 columns at pH 6.8 and 7.4. The relationship between Ve and molecular weight was similar over the pH range 4.0 to 8.0. The molec...

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Autor principal: Del C. Battle, A.M.
Formato: JOUR
Materias:
protein
animal
article
cattle
gel chromatography
molecular weight
Animal
Cattle
Chromatography, Gel
Molecular Weight
Proteins
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00219673_v28_nC_p82_DelCBattle
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00219673_v28_nC_p82_DelCBattle
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spelling todo:paper_00219673_v28_nC_p82_DelCBattle2023-10-03T14:24:59Z Estimation of molecular weights of proteins by bio-gel P gel filtration Del C. Battle, A.M. protein animal article cattle gel chromatography molecular weight Animal Cattle Chromatography, Gel Molecular Weight Proteins Correlation between elution volumes, Ve, and molecular weight was investigated by Bio-Gel filtration of proteins of known molecular weight on Bio-Gel P-100, P-200 and P-300 columns at pH 6.8 and 7.4. The relationship between Ve and molecular weight was similar over the pH range 4.0 to 8.0. The molecular weights of two enzymes, coproporphyrinogenase and δ-aminolaevulinic acid dehydrase, were estimated on calibrated Bio-Gel P-200 columns; their respective values were in agreement with those obtained by using calibrated Sephadex columns. The uncertainty in values estimated by this procedure was put at 12%. © 1967. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00219673_v28_nC_p82_DelCBattle
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic protein
animal
article
cattle
gel chromatography
molecular weight
Animal
Cattle
Chromatography, Gel
Molecular Weight
Proteins
spellingShingle protein
animal
article
cattle
gel chromatography
molecular weight
Animal
Cattle
Chromatography, Gel
Molecular Weight
Proteins
Del C. Battle, A.M.
Estimation of molecular weights of proteins by bio-gel P gel filtration
topic_facet protein
animal
article
cattle
gel chromatography
molecular weight
Animal
Cattle
Chromatography, Gel
Molecular Weight
Proteins
description Correlation between elution volumes, Ve, and molecular weight was investigated by Bio-Gel filtration of proteins of known molecular weight on Bio-Gel P-100, P-200 and P-300 columns at pH 6.8 and 7.4. The relationship between Ve and molecular weight was similar over the pH range 4.0 to 8.0. The molecular weights of two enzymes, coproporphyrinogenase and δ-aminolaevulinic acid dehydrase, were estimated on calibrated Bio-Gel P-200 columns; their respective values were in agreement with those obtained by using calibrated Sephadex columns. The uncertainty in values estimated by this procedure was put at 12%. © 1967.
format JOUR
author Del C. Battle, A.M.
author_facet Del C. Battle, A.M.
author_sort Del C. Battle, A.M.
title Estimation of molecular weights of proteins by bio-gel P gel filtration
title_short Estimation of molecular weights of proteins by bio-gel P gel filtration
title_full Estimation of molecular weights of proteins by bio-gel P gel filtration
title_fullStr Estimation of molecular weights of proteins by bio-gel P gel filtration
title_full_unstemmed Estimation of molecular weights of proteins by bio-gel P gel filtration
title_sort estimation of molecular weights of proteins by bio-gel p gel filtration
url http://hdl.handle.net/20.500.12110/paper_00219673_v28_nC_p82_DelCBattle
work_keys_str_mv AT delcbattleam estimationofmolecularweightsofproteinsbybiogelpgelfiltration
_version_ 1807321972623802368

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