Thermal-ultraviolet method for differential detection and recovery of organic compounds in thin-layer chromatography
Extending work on the detection of organic compounds in thin-layer chromatography using the thermal-ultraviolet method, the differential detection of sugars based on a temperature-dependent characteristic pattern revealed under UV and visible light is reported. Specific colours related to structural...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00219673_v265_nC_p193_Alperin |
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todo:paper_00219673_v265_nC_p193_Alperin2023-10-03T14:24:59Z Thermal-ultraviolet method for differential detection and recovery of organic compounds in thin-layer chromatography Alperin, D.M. Carminatti, H. Idoyaga-Vargas, V. Couso, R.O. Extending work on the detection of organic compounds in thin-layer chromatography using the thermal-ultraviolet method, the differential detection of sugars based on a temperature-dependent characteristic pattern revealed under UV and visible light is reported. Specific colours related to structural changes have been observed under UV light. High recoveries of 14C- and also 3H-labelled compounds after thermal-UV detection have been obtained. The detection of lipids and the determination of the sensitivity of the method for these compounds and carbohydrates are described. Results comparable to those in sulphuric acid-based methods have been obtained. In addition, the method has the advantage of a very low background even after prolonged periods of heating at high temperatures. The detection, differentiation and recovery of several methyl glucosides is also reported. © 1983. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00219673_v265_nC_p193_Alperin |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| description |
Extending work on the detection of organic compounds in thin-layer chromatography using the thermal-ultraviolet method, the differential detection of sugars based on a temperature-dependent characteristic pattern revealed under UV and visible light is reported. Specific colours related to structural changes have been observed under UV light. High recoveries of 14C- and also 3H-labelled compounds after thermal-UV detection have been obtained. The detection of lipids and the determination of the sensitivity of the method for these compounds and carbohydrates are described. Results comparable to those in sulphuric acid-based methods have been obtained. In addition, the method has the advantage of a very low background even after prolonged periods of heating at high temperatures. The detection, differentiation and recovery of several methyl glucosides is also reported. © 1983. |
| format |
JOUR |
| author |
Alperin, D.M. Carminatti, H. Idoyaga-Vargas, V. Couso, R.O. |
| spellingShingle |
Alperin, D.M. Carminatti, H. Idoyaga-Vargas, V. Couso, R.O. Thermal-ultraviolet method for differential detection and recovery of organic compounds in thin-layer chromatography |
| author_facet |
Alperin, D.M. Carminatti, H. Idoyaga-Vargas, V. Couso, R.O. |
| author_sort |
Alperin, D.M. |
| title |
Thermal-ultraviolet method for differential detection and recovery of organic compounds in thin-layer chromatography |
| title_short |
Thermal-ultraviolet method for differential detection and recovery of organic compounds in thin-layer chromatography |
| title_full |
Thermal-ultraviolet method for differential detection and recovery of organic compounds in thin-layer chromatography |
| title_fullStr |
Thermal-ultraviolet method for differential detection and recovery of organic compounds in thin-layer chromatography |
| title_full_unstemmed |
Thermal-ultraviolet method for differential detection and recovery of organic compounds in thin-layer chromatography |
| title_sort |
thermal-ultraviolet method for differential detection and recovery of organic compounds in thin-layer chromatography |
| url |
http://hdl.handle.net/20.500.12110/paper_00219673_v265_nC_p193_Alperin |
| work_keys_str_mv |
AT alperindm thermalultravioletmethodfordifferentialdetectionandrecoveryoforganiccompoundsinthinlayerchromatography AT carminattih thermalultravioletmethodfordifferentialdetectionandrecoveryoforganiccompoundsinthinlayerchromatography AT idoyagavargasv thermalultravioletmethodfordifferentialdetectionandrecoveryoforganiccompoundsinthinlayerchromatography AT cousoro thermalultravioletmethodfordifferentialdetectionandrecoveryoforganiccompoundsinthinlayerchromatography |
| _version_ |
1807323519023841280 |