Synthesis and characterization of molecularly imprinted polymer nanoparticles for coenzyme Q10 dispersive micro solid phase extraction
Molecularly imprinted polymer nanoparticles (MIPNPs) with the ability to recognize coenzyme Q10 (CoQ10) were synthesised in order to be employed as sorbent in a dispersive micro-solid phase extraction (DMSPE) for the determination of CoQ10 in a liver extract. CoQ10 is a redox-active, lipophilic subs...
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00219673_v1456_n_p1_Contin |
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todo:paper_00219673_v1456_n_p1_Contin |
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dspace |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Coenzyme Q10 Dispersive micro solid phase extraction Molecularly imprinted polymer nanoparticles Capillary electrophoresis Coenzymes Ethylene Ethylene glycol Infrared spectroscopy Light scattering Nanoparticles Phase separation Redox reactions Sorption Synthesis (chemical) Coenzyme Q10 Dispersive micro solid-phase extractions Ethylene glycol dimethacrylate Mitochondrial respiratory chain Molecularly Imprinted Polymer Precipitation polymerizations Synthesis and characterizations Ubiquinol-cytochrome c reductase Extraction acetonitrile ethylene glycol dimethacrylate liver extract methacrylic acid molecularly imprinted polymer nanoparticle peroxydisulfate potassium ubidecarenone water cross linking reagent methacrylic acid derivative nanoparticle polymethacrylic acid polymethacrylic acid derivative ubidecarenone ubiquinone adsorption animal tissue Article capillary electrophoresis controlled study desorption dispersive micro solid phase extraction electron microscopy gel mobility shift assay infrared spectroscopy limit of detection limit of quantitation nonhuman particle size photon correlation spectroscopy polymerization precipitation priority journal solid phase extraction zeta potential analogs and derivatives animal bovine chemistry isolation and purification liver molecular imprinting procedures solid phase extraction synthesis Adsorption Animals Cattle Cross-Linking Reagents Liver Methacrylates Molecular Imprinting Nanoparticles Polymerization Polymethacrylic Acids Solid Phase Extraction Ubiquinone |
| spellingShingle |
Coenzyme Q10 Dispersive micro solid phase extraction Molecularly imprinted polymer nanoparticles Capillary electrophoresis Coenzymes Ethylene Ethylene glycol Infrared spectroscopy Light scattering Nanoparticles Phase separation Redox reactions Sorption Synthesis (chemical) Coenzyme Q10 Dispersive micro solid-phase extractions Ethylene glycol dimethacrylate Mitochondrial respiratory chain Molecularly Imprinted Polymer Precipitation polymerizations Synthesis and characterizations Ubiquinol-cytochrome c reductase Extraction acetonitrile ethylene glycol dimethacrylate liver extract methacrylic acid molecularly imprinted polymer nanoparticle peroxydisulfate potassium ubidecarenone water cross linking reagent methacrylic acid derivative nanoparticle polymethacrylic acid polymethacrylic acid derivative ubidecarenone ubiquinone adsorption animal tissue Article capillary electrophoresis controlled study desorption dispersive micro solid phase extraction electron microscopy gel mobility shift assay infrared spectroscopy limit of detection limit of quantitation nonhuman particle size photon correlation spectroscopy polymerization precipitation priority journal solid phase extraction zeta potential analogs and derivatives animal bovine chemistry isolation and purification liver molecular imprinting procedures solid phase extraction synthesis Adsorption Animals Cattle Cross-Linking Reagents Liver Methacrylates Molecular Imprinting Nanoparticles Polymerization Polymethacrylic Acids Solid Phase Extraction Ubiquinone Contin, M. Bonelli, P. Lucangioli, S. Cukierman, A. Tripodi, V. Synthesis and characterization of molecularly imprinted polymer nanoparticles for coenzyme Q10 dispersive micro solid phase extraction |
| topic_facet |
Coenzyme Q10 Dispersive micro solid phase extraction Molecularly imprinted polymer nanoparticles Capillary electrophoresis Coenzymes Ethylene Ethylene glycol Infrared spectroscopy Light scattering Nanoparticles Phase separation Redox reactions Sorption Synthesis (chemical) Coenzyme Q10 Dispersive micro solid-phase extractions Ethylene glycol dimethacrylate Mitochondrial respiratory chain Molecularly Imprinted Polymer Precipitation polymerizations Synthesis and characterizations Ubiquinol-cytochrome c reductase Extraction acetonitrile ethylene glycol dimethacrylate liver extract methacrylic acid molecularly imprinted polymer nanoparticle peroxydisulfate potassium ubidecarenone water cross linking reagent methacrylic acid derivative nanoparticle polymethacrylic acid polymethacrylic acid derivative ubidecarenone ubiquinone adsorption animal tissue Article capillary electrophoresis controlled study desorption dispersive micro solid phase extraction electron microscopy gel mobility shift assay infrared spectroscopy limit of detection limit of quantitation nonhuman particle size photon correlation spectroscopy polymerization precipitation priority journal solid phase extraction zeta potential analogs and derivatives animal bovine chemistry isolation and purification liver molecular imprinting procedures solid phase extraction synthesis Adsorption Animals Cattle Cross-Linking Reagents Liver Methacrylates Molecular Imprinting Nanoparticles Polymerization Polymethacrylic Acids Solid Phase Extraction Ubiquinone |
| description |
Molecularly imprinted polymer nanoparticles (MIPNPs) with the ability to recognize coenzyme Q10 (CoQ10) were synthesised in order to be employed as sorbent in a dispersive micro-solid phase extraction (DMSPE) for the determination of CoQ10 in a liver extract. CoQ10 is a redox-active, lipophilic substance integrated in the mitochondrial respiratory chain which acts as an electron carrier, shuttling electrons from complex I (NADH-ubiquinone oxidoreductase) and II (succinate-ubiquinone oxidoreductase) to complex III (ubiquinol-cytochrome c reductase), for the production of cellular energy. The MIPNPs were synthesised by precipitation polymerization using coenzyme Q0 as the dummy template, methacrylic acid as the functional monomer, an acetonitrile: water mixture as the porogen, ethylene glycol dimethacrylate as the crosslinker and potassium persulfate as initiator. The nanoparticles were characterized by microscopy, capillary electrophoresis, dynamic light scattering, N2 adsorption–desorption isotherms, and infrared spectroscopy. The MIPNPs demonstrated the presence of selective cavities complementary to the quinone nucleus of CoQ10, leading to a specific recognition of CoQ10 compared with related compounds. In the liver extract the relative CoQ10 peak area (CoQ10 area/total peak area) increased from 4.6% to 25.4% after the DMSPE procedure. The recovery percentage of CoQ10 from the liver matrix was between 70.5% and 83.7% quantified against CoQ10 standard processed under the same conditions. The DMSPE procedure allows the elution of almost all the CoQ10 retained (99.4%) in a small volume (200 μL), allowing the sample to be concentrated 2.5 times (LOD: 1.1 μg g−1 and LOQ: 3.7 μg g−1 of tissue). The resulted clean up of the sample, the improvement in peak shape and baseline and the reduction of interferences, evidence that the MIPNPs could potentially be applied as sorbent in a DMSPE with satisfactory results and with a minimum amount of sorbent (1 mg). © 2016 Elsevier B.V. |
| format |
JOUR |
| author |
Contin, M. Bonelli, P. Lucangioli, S. Cukierman, A. Tripodi, V. |
| author_facet |
Contin, M. Bonelli, P. Lucangioli, S. Cukierman, A. Tripodi, V. |
| author_sort |
Contin, M. |
| title |
Synthesis and characterization of molecularly imprinted polymer nanoparticles for coenzyme Q10 dispersive micro solid phase extraction |
| title_short |
Synthesis and characterization of molecularly imprinted polymer nanoparticles for coenzyme Q10 dispersive micro solid phase extraction |
| title_full |
Synthesis and characterization of molecularly imprinted polymer nanoparticles for coenzyme Q10 dispersive micro solid phase extraction |
| title_fullStr |
Synthesis and characterization of molecularly imprinted polymer nanoparticles for coenzyme Q10 dispersive micro solid phase extraction |
| title_full_unstemmed |
Synthesis and characterization of molecularly imprinted polymer nanoparticles for coenzyme Q10 dispersive micro solid phase extraction |
| title_sort |
synthesis and characterization of molecularly imprinted polymer nanoparticles for coenzyme q10 dispersive micro solid phase extraction |
| url |
http://hdl.handle.net/20.500.12110/paper_00219673_v1456_n_p1_Contin |
| work_keys_str_mv |
AT continm synthesisandcharacterizationofmolecularlyimprintedpolymernanoparticlesforcoenzymeq10dispersivemicrosolidphaseextraction AT bonellip synthesisandcharacterizationofmolecularlyimprintedpolymernanoparticlesforcoenzymeq10dispersivemicrosolidphaseextraction AT lucangiolis synthesisandcharacterizationofmolecularlyimprintedpolymernanoparticlesforcoenzymeq10dispersivemicrosolidphaseextraction AT cukiermana synthesisandcharacterizationofmolecularlyimprintedpolymernanoparticlesforcoenzymeq10dispersivemicrosolidphaseextraction AT tripodiv synthesisandcharacterizationofmolecularlyimprintedpolymernanoparticlesforcoenzymeq10dispersivemicrosolidphaseextraction |
| _version_ |
1807318874129956864 |
| spelling |
todo:paper_00219673_v1456_n_p1_Contin2023-10-03T14:24:56Z Synthesis and characterization of molecularly imprinted polymer nanoparticles for coenzyme Q10 dispersive micro solid phase extraction Contin, M. Bonelli, P. Lucangioli, S. Cukierman, A. Tripodi, V. Coenzyme Q10 Dispersive micro solid phase extraction Molecularly imprinted polymer nanoparticles Capillary electrophoresis Coenzymes Ethylene Ethylene glycol Infrared spectroscopy Light scattering Nanoparticles Phase separation Redox reactions Sorption Synthesis (chemical) Coenzyme Q10 Dispersive micro solid-phase extractions Ethylene glycol dimethacrylate Mitochondrial respiratory chain Molecularly Imprinted Polymer Precipitation polymerizations Synthesis and characterizations Ubiquinol-cytochrome c reductase Extraction acetonitrile ethylene glycol dimethacrylate liver extract methacrylic acid molecularly imprinted polymer nanoparticle peroxydisulfate potassium ubidecarenone water cross linking reagent methacrylic acid derivative nanoparticle polymethacrylic acid polymethacrylic acid derivative ubidecarenone ubiquinone adsorption animal tissue Article capillary electrophoresis controlled study desorption dispersive micro solid phase extraction electron microscopy gel mobility shift assay infrared spectroscopy limit of detection limit of quantitation nonhuman particle size photon correlation spectroscopy polymerization precipitation priority journal solid phase extraction zeta potential analogs and derivatives animal bovine chemistry isolation and purification liver molecular imprinting procedures solid phase extraction synthesis Adsorption Animals Cattle Cross-Linking Reagents Liver Methacrylates Molecular Imprinting Nanoparticles Polymerization Polymethacrylic Acids Solid Phase Extraction Ubiquinone Molecularly imprinted polymer nanoparticles (MIPNPs) with the ability to recognize coenzyme Q10 (CoQ10) were synthesised in order to be employed as sorbent in a dispersive micro-solid phase extraction (DMSPE) for the determination of CoQ10 in a liver extract. CoQ10 is a redox-active, lipophilic substance integrated in the mitochondrial respiratory chain which acts as an electron carrier, shuttling electrons from complex I (NADH-ubiquinone oxidoreductase) and II (succinate-ubiquinone oxidoreductase) to complex III (ubiquinol-cytochrome c reductase), for the production of cellular energy. The MIPNPs were synthesised by precipitation polymerization using coenzyme Q0 as the dummy template, methacrylic acid as the functional monomer, an acetonitrile: water mixture as the porogen, ethylene glycol dimethacrylate as the crosslinker and potassium persulfate as initiator. The nanoparticles were characterized by microscopy, capillary electrophoresis, dynamic light scattering, N2 adsorption–desorption isotherms, and infrared spectroscopy. The MIPNPs demonstrated the presence of selective cavities complementary to the quinone nucleus of CoQ10, leading to a specific recognition of CoQ10 compared with related compounds. In the liver extract the relative CoQ10 peak area (CoQ10 area/total peak area) increased from 4.6% to 25.4% after the DMSPE procedure. The recovery percentage of CoQ10 from the liver matrix was between 70.5% and 83.7% quantified against CoQ10 standard processed under the same conditions. The DMSPE procedure allows the elution of almost all the CoQ10 retained (99.4%) in a small volume (200 μL), allowing the sample to be concentrated 2.5 times (LOD: 1.1 μg g−1 and LOQ: 3.7 μg g−1 of tissue). The resulted clean up of the sample, the improvement in peak shape and baseline and the reduction of interferences, evidence that the MIPNPs could potentially be applied as sorbent in a DMSPE with satisfactory results and with a minimum amount of sorbent (1 mg). © 2016 Elsevier B.V. Fil:Bonelli, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cukierman, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00219673_v1456_n_p1_Contin |