Assay of caffeoylquinic acids in Baccharis trimera by reversed-phase liquid chromatography
Baccharis trimera commonly named 'carqueja', is wide-spread in South America and are used as raw material for herbal medicines. A reversed-phase liquid chromatography (RP-LC) method coupled to diode array detector was developed for the analysis of caffeoylquinic acids (CQAs), the main comp...
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todo:paper_00219673_v1219_n_p147_Aboy2023-10-03T14:24:55Z Assay of caffeoylquinic acids in Baccharis trimera by reversed-phase liquid chromatography Aboy, A.L. Apel, M.A. Debenedetti, S. Francescato, L. Rosella, M.A. Henriques, A.T. Baccharis trimera Caffeoylquinic acids Dicaffeoylquinic acids Photostability RP-LC Tricaffeoylquinic acids Baccharis trimera Caffeoylquinic acids Diode array detectors Herbal medicines HPLC method Limits of detection Photo-stability Plant material Quinic acids Reversed-phase liquid chromatography RP-LC South America Validation data Water baths Ethanol Liquids Mass spectrometry Organic solvents Plant extracts Liquid chromatography 3,4 o dicaffeoylquinic acid 3,5 o dicaffeoylquinic acid 4,5 o dicaffeoylquinic acid 5 o caffeoylquinic acid alcohol caffeoylquinic acid derivative plant extract plant medicinal product tricaffeoylquinic acid unclassified drug water accuracy article Baccharis Baccharis trimera controlled study devices diode array detector limit of detection limit of quantitation linear system mass spectrometry nonhuman physical parameters priority journal reversed phase liquid chromatography seasonal variation soil validation study Argentina Baccharis Brazil Chromatography, High Pressure Liquid Chromatography, Reverse-Phase Drug Stability Limit of Detection Photochemical Processes Plant Extracts Quinic Acid Reproducibility of Results Tandem Mass Spectrometry Baccharis trimera Baccharis trimera commonly named 'carqueja', is wide-spread in South America and are used as raw material for herbal medicines. A reversed-phase liquid chromatography (RP-LC) method coupled to diode array detector was developed for the analysis of caffeoylquinic acids (CQAs), the main compounds responsible for its digestive activity. The identity of the quinic acids was established by mass spectrometry and were them: 5-O-[E]-caffeoylquinic acid, 3,4-O-[E]-dicaffeoylquinic acid, 3,5-O-[E]-dicaffeoylquinic acid, 4,5-O-[E]-dicaffeoylquinic acid and a tricaffeoylquinic acid. The RP-LC method for the quantitation of the caffeoylquinic acids was validated according to ICH guidelines, based on the following parameters: linearity, selectivity, robustness, limits of detection and quantification, precision and recovery. Hydroalcoholic extracts were prepared by the maceration of the plant material with ethanol:water 1:1 (v/v) in a 0.1:25gmL -1 plant:solvent ratio in a water bath at 40°C. Validation data indicated that the HPLC method proposed is suitable for the analysis of caffeoylquinic acids in B. trimera raw material. The results of the LOD and LOQ analyses for the 5-CQA were 4.1μgmL -1 and 12.5μgmL -1, respectively, 1.3μgmL -1, 3.9μgmL -1 for 4,5-diCQA and 1.7μgmL -1, 5.1μgmL -1 for triCQA. The levels of total CQAs ranged from 2.1 to 4.0g% (w/w). The influence of season harvest and site collection was also evaluated and variations were observed in the results and can be related to phonologic phase, different locations, seasons and soil. Long term and photostability of plant material were carried out and was observed a stable behavior during the time of the experiments. © 2011 Elsevier B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00219673_v1219_n_p147_Aboy |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Baccharis trimera Caffeoylquinic acids Dicaffeoylquinic acids Photostability RP-LC Tricaffeoylquinic acids Baccharis trimera Caffeoylquinic acids Diode array detectors Herbal medicines HPLC method Limits of detection Photo-stability Plant material Quinic acids Reversed-phase liquid chromatography RP-LC South America Validation data Water baths Ethanol Liquids Mass spectrometry Organic solvents Plant extracts Liquid chromatography 3,4 o dicaffeoylquinic acid 3,5 o dicaffeoylquinic acid 4,5 o dicaffeoylquinic acid 5 o caffeoylquinic acid alcohol caffeoylquinic acid derivative plant extract plant medicinal product tricaffeoylquinic acid unclassified drug water accuracy article Baccharis Baccharis trimera controlled study devices diode array detector limit of detection limit of quantitation linear system mass spectrometry nonhuman physical parameters priority journal reversed phase liquid chromatography seasonal variation soil validation study Argentina Baccharis Brazil Chromatography, High Pressure Liquid Chromatography, Reverse-Phase Drug Stability Limit of Detection Photochemical Processes Plant Extracts Quinic Acid Reproducibility of Results Tandem Mass Spectrometry Baccharis trimera |
| spellingShingle |
Baccharis trimera Caffeoylquinic acids Dicaffeoylquinic acids Photostability RP-LC Tricaffeoylquinic acids Baccharis trimera Caffeoylquinic acids Diode array detectors Herbal medicines HPLC method Limits of detection Photo-stability Plant material Quinic acids Reversed-phase liquid chromatography RP-LC South America Validation data Water baths Ethanol Liquids Mass spectrometry Organic solvents Plant extracts Liquid chromatography 3,4 o dicaffeoylquinic acid 3,5 o dicaffeoylquinic acid 4,5 o dicaffeoylquinic acid 5 o caffeoylquinic acid alcohol caffeoylquinic acid derivative plant extract plant medicinal product tricaffeoylquinic acid unclassified drug water accuracy article Baccharis Baccharis trimera controlled study devices diode array detector limit of detection limit of quantitation linear system mass spectrometry nonhuman physical parameters priority journal reversed phase liquid chromatography seasonal variation soil validation study Argentina Baccharis Brazil Chromatography, High Pressure Liquid Chromatography, Reverse-Phase Drug Stability Limit of Detection Photochemical Processes Plant Extracts Quinic Acid Reproducibility of Results Tandem Mass Spectrometry Baccharis trimera Aboy, A.L. Apel, M.A. Debenedetti, S. Francescato, L. Rosella, M.A. Henriques, A.T. Assay of caffeoylquinic acids in Baccharis trimera by reversed-phase liquid chromatography |
| topic_facet |
Baccharis trimera Caffeoylquinic acids Dicaffeoylquinic acids Photostability RP-LC Tricaffeoylquinic acids Baccharis trimera Caffeoylquinic acids Diode array detectors Herbal medicines HPLC method Limits of detection Photo-stability Plant material Quinic acids Reversed-phase liquid chromatography RP-LC South America Validation data Water baths Ethanol Liquids Mass spectrometry Organic solvents Plant extracts Liquid chromatography 3,4 o dicaffeoylquinic acid 3,5 o dicaffeoylquinic acid 4,5 o dicaffeoylquinic acid 5 o caffeoylquinic acid alcohol caffeoylquinic acid derivative plant extract plant medicinal product tricaffeoylquinic acid unclassified drug water accuracy article Baccharis Baccharis trimera controlled study devices diode array detector limit of detection limit of quantitation linear system mass spectrometry nonhuman physical parameters priority journal reversed phase liquid chromatography seasonal variation soil validation study Argentina Baccharis Brazil Chromatography, High Pressure Liquid Chromatography, Reverse-Phase Drug Stability Limit of Detection Photochemical Processes Plant Extracts Quinic Acid Reproducibility of Results Tandem Mass Spectrometry Baccharis trimera |
| description |
Baccharis trimera commonly named 'carqueja', is wide-spread in South America and are used as raw material for herbal medicines. A reversed-phase liquid chromatography (RP-LC) method coupled to diode array detector was developed for the analysis of caffeoylquinic acids (CQAs), the main compounds responsible for its digestive activity. The identity of the quinic acids was established by mass spectrometry and were them: 5-O-[E]-caffeoylquinic acid, 3,4-O-[E]-dicaffeoylquinic acid, 3,5-O-[E]-dicaffeoylquinic acid, 4,5-O-[E]-dicaffeoylquinic acid and a tricaffeoylquinic acid. The RP-LC method for the quantitation of the caffeoylquinic acids was validated according to ICH guidelines, based on the following parameters: linearity, selectivity, robustness, limits of detection and quantification, precision and recovery. Hydroalcoholic extracts were prepared by the maceration of the plant material with ethanol:water 1:1 (v/v) in a 0.1:25gmL -1 plant:solvent ratio in a water bath at 40°C. Validation data indicated that the HPLC method proposed is suitable for the analysis of caffeoylquinic acids in B. trimera raw material. The results of the LOD and LOQ analyses for the 5-CQA were 4.1μgmL -1 and 12.5μgmL -1, respectively, 1.3μgmL -1, 3.9μgmL -1 for 4,5-diCQA and 1.7μgmL -1, 5.1μgmL -1 for triCQA. The levels of total CQAs ranged from 2.1 to 4.0g% (w/w). The influence of season harvest and site collection was also evaluated and variations were observed in the results and can be related to phonologic phase, different locations, seasons and soil. Long term and photostability of plant material were carried out and was observed a stable behavior during the time of the experiments. © 2011 Elsevier B.V. |
| format |
JOUR |
| author |
Aboy, A.L. Apel, M.A. Debenedetti, S. Francescato, L. Rosella, M.A. Henriques, A.T. |
| author_facet |
Aboy, A.L. Apel, M.A. Debenedetti, S. Francescato, L. Rosella, M.A. Henriques, A.T. |
| author_sort |
Aboy, A.L. |
| title |
Assay of caffeoylquinic acids in Baccharis trimera by reversed-phase liquid chromatography |
| title_short |
Assay of caffeoylquinic acids in Baccharis trimera by reversed-phase liquid chromatography |
| title_full |
Assay of caffeoylquinic acids in Baccharis trimera by reversed-phase liquid chromatography |
| title_fullStr |
Assay of caffeoylquinic acids in Baccharis trimera by reversed-phase liquid chromatography |
| title_full_unstemmed |
Assay of caffeoylquinic acids in Baccharis trimera by reversed-phase liquid chromatography |
| title_sort |
assay of caffeoylquinic acids in baccharis trimera by reversed-phase liquid chromatography |
| url |
http://hdl.handle.net/20.500.12110/paper_00219673_v1219_n_p147_Aboy |
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