Cyclic AMP inhibits fibronectin gene expression in a newly developed granulosa cell line by a mechanism that suppresses cAMP-responsive element-dependent transcriptional activation
The main role of the ovarian granulosa cells is to nurse the oocyte and to produce estradiol and progesterone upon stimulation by gonadotropins. In fact, follicle-stimulating hormone (FSH) and luteinizing hormone control the expression of several genes during granulosa cell differentiation via cycli...
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todo:paper_00219258_v265_n30_p18219_Bernath2023-10-03T14:22:56Z Cyclic AMP inhibits fibronectin gene expression in a newly developed granulosa cell line by a mechanism that suppresses cAMP-responsive element-dependent transcriptional activation Bernath, V.A. Muro, A.F. Vitullo, A.D. Bley, M.A. Barañao, J.L. Kornblihtt, A.R. cyclic amp dna binding protein fibronectin animal cell article cattle cell culture cyclic amp responsive element gene expression regulation genetic transcription granulosa cell nonhuman priority journal Animal Base Sequence Binding Sites Blotting, Northern Bucladesine Cattle Cell Line Cyclic AMP DNA-Binding Proteins Estradiol Female Fibronectins Follicle Stimulating Hormone Forskolin Gene Expression Regulation Granulosa Cells Karyotyping Molecular Sequence Data Nuclear Proteins Promoter Regions (Genetics) Regulatory Sequences, Nucleic Acid RNA, Messenger Support, Non-U.S. Gov't Transcription, Genetic Animalia Bos taurus Bovinae The main role of the ovarian granulosa cells is to nurse the oocyte and to produce estradiol and progesterone upon stimulation by gonadotropins. In fact, follicle-stimulating hormone (FSH) and luteinizing hormone control the expression of several genes during granulosa cell differentiation via cyclic AMP-dependent phosphorylations. Cyclic AMP stimulates transcription of genes that carry the cAMP-responsive element (CRE,5'TGACGTCA3') in their promoters. The fibronectin (FN) gene contains one CRE sequence at position -170. However, gonadotropins and cAMP inhibit FN gene expression in granulosa cells. To study the mechanism of the inhibition we developed a bovine granulosa cell line (BGC-1) that synthesizes estradiol in response to FSH and in which FSH and dibutyryl cAMP specifically decrease FN synthesis and its mRNA levels. The inhibitory effect (a) is not due to an alternation in FN mRNA stability, (b) requires upstream sequences other than CRE, located between positions -510 and -223, that are able to bind granulosa cell nuclear proteins, (c) is entirely dependent on the synthesis of intermediate proteins induced and or phosphorylated by cAMP, and (d) effectively suppresses the CRE-dependent transcriptional activation. Fil:Bernath, V.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Muro, A.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vitullo, A.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bley, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barañao, J.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00219258_v265_n30_p18219_Bernath |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cyclic amp dna binding protein fibronectin animal cell article cattle cell culture cyclic amp responsive element gene expression regulation genetic transcription granulosa cell nonhuman priority journal Animal Base Sequence Binding Sites Blotting, Northern Bucladesine Cattle Cell Line Cyclic AMP DNA-Binding Proteins Estradiol Female Fibronectins Follicle Stimulating Hormone Forskolin Gene Expression Regulation Granulosa Cells Karyotyping Molecular Sequence Data Nuclear Proteins Promoter Regions (Genetics) Regulatory Sequences, Nucleic Acid RNA, Messenger Support, Non-U.S. Gov't Transcription, Genetic Animalia Bos taurus Bovinae |
| spellingShingle |
cyclic amp dna binding protein fibronectin animal cell article cattle cell culture cyclic amp responsive element gene expression regulation genetic transcription granulosa cell nonhuman priority journal Animal Base Sequence Binding Sites Blotting, Northern Bucladesine Cattle Cell Line Cyclic AMP DNA-Binding Proteins Estradiol Female Fibronectins Follicle Stimulating Hormone Forskolin Gene Expression Regulation Granulosa Cells Karyotyping Molecular Sequence Data Nuclear Proteins Promoter Regions (Genetics) Regulatory Sequences, Nucleic Acid RNA, Messenger Support, Non-U.S. Gov't Transcription, Genetic Animalia Bos taurus Bovinae Bernath, V.A. Muro, A.F. Vitullo, A.D. Bley, M.A. Barañao, J.L. Kornblihtt, A.R. Cyclic AMP inhibits fibronectin gene expression in a newly developed granulosa cell line by a mechanism that suppresses cAMP-responsive element-dependent transcriptional activation |
| topic_facet |
cyclic amp dna binding protein fibronectin animal cell article cattle cell culture cyclic amp responsive element gene expression regulation genetic transcription granulosa cell nonhuman priority journal Animal Base Sequence Binding Sites Blotting, Northern Bucladesine Cattle Cell Line Cyclic AMP DNA-Binding Proteins Estradiol Female Fibronectins Follicle Stimulating Hormone Forskolin Gene Expression Regulation Granulosa Cells Karyotyping Molecular Sequence Data Nuclear Proteins Promoter Regions (Genetics) Regulatory Sequences, Nucleic Acid RNA, Messenger Support, Non-U.S. Gov't Transcription, Genetic Animalia Bos taurus Bovinae |
| description |
The main role of the ovarian granulosa cells is to nurse the oocyte and to produce estradiol and progesterone upon stimulation by gonadotropins. In fact, follicle-stimulating hormone (FSH) and luteinizing hormone control the expression of several genes during granulosa cell differentiation via cyclic AMP-dependent phosphorylations. Cyclic AMP stimulates transcription of genes that carry the cAMP-responsive element (CRE,5'TGACGTCA3') in their promoters. The fibronectin (FN) gene contains one CRE sequence at position -170. However, gonadotropins and cAMP inhibit FN gene expression in granulosa cells. To study the mechanism of the inhibition we developed a bovine granulosa cell line (BGC-1) that synthesizes estradiol in response to FSH and in which FSH and dibutyryl cAMP specifically decrease FN synthesis and its mRNA levels. The inhibitory effect (a) is not due to an alternation in FN mRNA stability, (b) requires upstream sequences other than CRE, located between positions -510 and -223, that are able to bind granulosa cell nuclear proteins, (c) is entirely dependent on the synthesis of intermediate proteins induced and or phosphorylated by cAMP, and (d) effectively suppresses the CRE-dependent transcriptional activation. |
| format |
JOUR |
| author |
Bernath, V.A. Muro, A.F. Vitullo, A.D. Bley, M.A. Barañao, J.L. Kornblihtt, A.R. |
| author_facet |
Bernath, V.A. Muro, A.F. Vitullo, A.D. Bley, M.A. Barañao, J.L. Kornblihtt, A.R. |
| author_sort |
Bernath, V.A. |
| title |
Cyclic AMP inhibits fibronectin gene expression in a newly developed granulosa cell line by a mechanism that suppresses cAMP-responsive element-dependent transcriptional activation |
| title_short |
Cyclic AMP inhibits fibronectin gene expression in a newly developed granulosa cell line by a mechanism that suppresses cAMP-responsive element-dependent transcriptional activation |
| title_full |
Cyclic AMP inhibits fibronectin gene expression in a newly developed granulosa cell line by a mechanism that suppresses cAMP-responsive element-dependent transcriptional activation |
| title_fullStr |
Cyclic AMP inhibits fibronectin gene expression in a newly developed granulosa cell line by a mechanism that suppresses cAMP-responsive element-dependent transcriptional activation |
| title_full_unstemmed |
Cyclic AMP inhibits fibronectin gene expression in a newly developed granulosa cell line by a mechanism that suppresses cAMP-responsive element-dependent transcriptional activation |
| title_sort |
cyclic amp inhibits fibronectin gene expression in a newly developed granulosa cell line by a mechanism that suppresses camp-responsive element-dependent transcriptional activation |
| url |
http://hdl.handle.net/20.500.12110/paper_00219258_v265_n30_p18219_Bernath |
| work_keys_str_mv |
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