The effect of organic solvents on the activation and the activity of spinach chloroplast fructose-1,6-bisphosphatase
A two-stage assay was used to study the effect of organic solvents on the activation of and the catalysis by chloroplast fructose-1,6-bisphosphatase. Irrespective of chemical structure, all the organic solvents tested had a dual effect on the enzyme. In the activation they stimulated and inhibited a...
Guardado en:
| Autores principales: | , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00219258_v260_n7_p3978_Corley |
| Aporte de: |
| id |
todo:paper_00219258_v260_n7_p3978_Corley |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_00219258_v260_n7_p3978_Corley2023-10-03T14:22:55Z The effect of organic solvents on the activation and the activity of spinach chloroplast fructose-1,6-bisphosphatase Corley, E. Wolosiuk, R.A. enzyme fructose bisphosphatase organic solvent chloroplast higher plant nonhuman priority journal 1-Propanol Calcium Chloroplasts Dithiothreitol Enzyme Activation Fructose-Bisphosphatase Fructosediphosphates Plant Proteins Solvents Support, Non-U.S. Gov't Temperature A two-stage assay was used to study the effect of organic solvents on the activation of and the catalysis by chloroplast fructose-1,6-bisphosphatase. Irrespective of chemical structure, all the organic solvents tested had a dual effect on the enzyme. In the activation they stimulated and inhibited at low and high concentrations, respectively, in a process that required dithiohreitol, fructose-1,6-bisphosphate, and Ca2+. Conversely, organic solvents inhibited catalysis. The enhancement in fructose-1,6-bisphosphatase activity did not arise from a change in the molecular weight of the enzyme and correlated positively with the hydrophobic character of the organic solvent. In the presence of 2-propranol, all the activation constants for modulators (fructose-1,6-bisphosphate, Ca2+, thioredoxin-f) were lower than in a strictly aqueous medium. Monothiols were also functional in the activation of chloroplast fructose-1,6-bisphosphatase, although they were less effective than dithiols. Sulfhydryl compounds decreased the concentration of fructose-1,6-bisphosphate required for the activation of the enzyme, and 2-propanol lowered this requirement further. Arrhenius plots were nonlinear for the enzyme activation and linear for the hydrolytic step. The anomalous temperature dependence of the chloroplast fructose-1,6-bisphosphatase activation was indicative of a cooperative process. The data obtained in this study indicate that the concerted activation of chloroplast fructose-1,6-bisphosphatase is favored in a medium less polar than water. Fil:Wolosiuk, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00219258_v260_n7_p3978_Corley |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
enzyme fructose bisphosphatase organic solvent chloroplast higher plant nonhuman priority journal 1-Propanol Calcium Chloroplasts Dithiothreitol Enzyme Activation Fructose-Bisphosphatase Fructosediphosphates Plant Proteins Solvents Support, Non-U.S. Gov't Temperature |
| spellingShingle |
enzyme fructose bisphosphatase organic solvent chloroplast higher plant nonhuman priority journal 1-Propanol Calcium Chloroplasts Dithiothreitol Enzyme Activation Fructose-Bisphosphatase Fructosediphosphates Plant Proteins Solvents Support, Non-U.S. Gov't Temperature Corley, E. Wolosiuk, R.A. The effect of organic solvents on the activation and the activity of spinach chloroplast fructose-1,6-bisphosphatase |
| topic_facet |
enzyme fructose bisphosphatase organic solvent chloroplast higher plant nonhuman priority journal 1-Propanol Calcium Chloroplasts Dithiothreitol Enzyme Activation Fructose-Bisphosphatase Fructosediphosphates Plant Proteins Solvents Support, Non-U.S. Gov't Temperature |
| description |
A two-stage assay was used to study the effect of organic solvents on the activation of and the catalysis by chloroplast fructose-1,6-bisphosphatase. Irrespective of chemical structure, all the organic solvents tested had a dual effect on the enzyme. In the activation they stimulated and inhibited at low and high concentrations, respectively, in a process that required dithiohreitol, fructose-1,6-bisphosphate, and Ca2+. Conversely, organic solvents inhibited catalysis. The enhancement in fructose-1,6-bisphosphatase activity did not arise from a change in the molecular weight of the enzyme and correlated positively with the hydrophobic character of the organic solvent. In the presence of 2-propranol, all the activation constants for modulators (fructose-1,6-bisphosphate, Ca2+, thioredoxin-f) were lower than in a strictly aqueous medium. Monothiols were also functional in the activation of chloroplast fructose-1,6-bisphosphatase, although they were less effective than dithiols. Sulfhydryl compounds decreased the concentration of fructose-1,6-bisphosphate required for the activation of the enzyme, and 2-propanol lowered this requirement further. Arrhenius plots were nonlinear for the enzyme activation and linear for the hydrolytic step. The anomalous temperature dependence of the chloroplast fructose-1,6-bisphosphatase activation was indicative of a cooperative process. The data obtained in this study indicate that the concerted activation of chloroplast fructose-1,6-bisphosphatase is favored in a medium less polar than water. |
| format |
JOUR |
| author |
Corley, E. Wolosiuk, R.A. |
| author_facet |
Corley, E. Wolosiuk, R.A. |
| author_sort |
Corley, E. |
| title |
The effect of organic solvents on the activation and the activity of spinach chloroplast fructose-1,6-bisphosphatase |
| title_short |
The effect of organic solvents on the activation and the activity of spinach chloroplast fructose-1,6-bisphosphatase |
| title_full |
The effect of organic solvents on the activation and the activity of spinach chloroplast fructose-1,6-bisphosphatase |
| title_fullStr |
The effect of organic solvents on the activation and the activity of spinach chloroplast fructose-1,6-bisphosphatase |
| title_full_unstemmed |
The effect of organic solvents on the activation and the activity of spinach chloroplast fructose-1,6-bisphosphatase |
| title_sort |
effect of organic solvents on the activation and the activity of spinach chloroplast fructose-1,6-bisphosphatase |
| url |
http://hdl.handle.net/20.500.12110/paper_00219258_v260_n7_p3978_Corley |
| work_keys_str_mv |
AT corleye theeffectoforganicsolventsontheactivationandtheactivityofspinachchloroplastfructose16bisphosphatase AT wolosiukra theeffectoforganicsolventsontheactivationandtheactivityofspinachchloroplastfructose16bisphosphatase AT corleye effectoforganicsolventsontheactivationandtheactivityofspinachchloroplastfructose16bisphosphatase AT wolosiukra effectoforganicsolventsontheactivationandtheactivityofspinachchloroplastfructose16bisphosphatase |
| _version_ |
1782027612568158208 |