Promoter analysis of the Xanthomonas campestris pv. campestris gum operon directing biosynthesis of the xanthan polysaccharide

The Xanthomonas campestris gum gene cluster is composed of 12 genes designated gumB, -C, -D, -E, -F, -G, -H, -I, -J, -K, -L, and -M. The transcriptional organization of this gene cluster was analyzed by the construction of gum-lacZ transcriptional fusions in association with plasmid integration muta...

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Autores principales: Katzen, F., Becker, A., Zorreguieta, A., Pühler, A., Ielpi, L.
Formato: JOUR
Materias:
xanthan
article
bacterial mutation
carbohydrate synthesis
controlled study
gene cluster
gene mapping
nonhuman
operon
priority journal
promoter region
transcription regulation
transposon
Xanthomonas
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00219193_v178_n14_p4313_Katzen
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00219193_v178_n14_p4313_Katzen
record_format dspace
spelling todo:paper_00219193_v178_n14_p4313_Katzen2023-10-03T14:22:47Z Promoter analysis of the Xanthomonas campestris pv. campestris gum operon directing biosynthesis of the xanthan polysaccharide Katzen, F. Becker, A. Zorreguieta, A. Pühler, A. Ielpi, L. xanthan article bacterial mutation carbohydrate synthesis controlled study gene cluster gene mapping nonhuman operon priority journal promoter region transcription regulation transposon Xanthomonas The Xanthomonas campestris gum gene cluster is composed of 12 genes designated gumB, -C, -D, -E, -F, -G, -H, -I, -J, -K, -L, and -M. The transcriptional organization of this gene cluster was analyzed by the construction of gum-lacZ transcriptional fusions in association with plasmid integration mutagenesis. This analysis, coupled with primer extension assays, indicated that the gum region was mainly expressed as an operon from a promoter located upstream of the first gene, gumB. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00219193_v178_n14_p4313_Katzen
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic xanthan
article
bacterial mutation
carbohydrate synthesis
controlled study
gene cluster
gene mapping
nonhuman
operon
priority journal
promoter region
transcription regulation
transposon
Xanthomonas
spellingShingle xanthan
article
bacterial mutation
carbohydrate synthesis
controlled study
gene cluster
gene mapping
nonhuman
operon
priority journal
promoter region
transcription regulation
transposon
Xanthomonas
Katzen, F.
Becker, A.
Zorreguieta, A.
Pühler, A.
Ielpi, L.
Promoter analysis of the Xanthomonas campestris pv. campestris gum operon directing biosynthesis of the xanthan polysaccharide
topic_facet xanthan
article
bacterial mutation
carbohydrate synthesis
controlled study
gene cluster
gene mapping
nonhuman
operon
priority journal
promoter region
transcription regulation
transposon
Xanthomonas
description The Xanthomonas campestris gum gene cluster is composed of 12 genes designated gumB, -C, -D, -E, -F, -G, -H, -I, -J, -K, -L, and -M. The transcriptional organization of this gene cluster was analyzed by the construction of gum-lacZ transcriptional fusions in association with plasmid integration mutagenesis. This analysis, coupled with primer extension assays, indicated that the gum region was mainly expressed as an operon from a promoter located upstream of the first gene, gumB.
format JOUR
author Katzen, F.
Becker, A.
Zorreguieta, A.
Pühler, A.
Ielpi, L.
author_facet Katzen, F.
Becker, A.
Zorreguieta, A.
Pühler, A.
Ielpi, L.
author_sort Katzen, F.
title Promoter analysis of the Xanthomonas campestris pv. campestris gum operon directing biosynthesis of the xanthan polysaccharide
title_short Promoter analysis of the Xanthomonas campestris pv. campestris gum operon directing biosynthesis of the xanthan polysaccharide
title_full Promoter analysis of the Xanthomonas campestris pv. campestris gum operon directing biosynthesis of the xanthan polysaccharide
title_fullStr Promoter analysis of the Xanthomonas campestris pv. campestris gum operon directing biosynthesis of the xanthan polysaccharide
title_full_unstemmed Promoter analysis of the Xanthomonas campestris pv. campestris gum operon directing biosynthesis of the xanthan polysaccharide
title_sort promoter analysis of the xanthomonas campestris pv. campestris gum operon directing biosynthesis of the xanthan polysaccharide
url http://hdl.handle.net/20.500.12110/paper_00219193_v178_n14_p4313_Katzen
work_keys_str_mv AT katzenf promoteranalysisofthexanthomonascampestrispvcampestrisgumoperondirectingbiosynthesisofthexanthanpolysaccharide
AT beckera promoteranalysisofthexanthomonascampestrispvcampestrisgumoperondirectingbiosynthesisofthexanthanpolysaccharide
AT zorreguietaa promoteranalysisofthexanthomonascampestrispvcampestrisgumoperondirectingbiosynthesisofthexanthanpolysaccharide
AT puhlera promoteranalysisofthexanthomonascampestrispvcampestrisgumoperondirectingbiosynthesisofthexanthanpolysaccharide
AT ielpil promoteranalysisofthexanthomonascampestrispvcampestrisgumoperondirectingbiosynthesisofthexanthanpolysaccharide
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