Expression of the Agrobacterium tumefaciens chvB virulence region in Azospirillum spp.

Inner membranes of Azospirillum brasilense incubated with UDP-glucose were unable to synthesize β-(1-2) glucan and lacked the 235-kilodalton intermediate protein known to be involved in the synthesis of β-(1-2) glucan in Agrobacterium tumefaciens and Rhizobium meliloti. Inner membranes of A. brasile...

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Autores principales:	Altabe, S., Inon de Iannino, N., De Mendoza, D., Ugalde, R.A.
Formato:	JOUR
Materias:	restriction endonuclease article Azospirillum gene expression genetic engineering membrane nonhuman priority journal Rhizobium radiobacter sequence homology virulence Agrobacterium tumefaciens Azospirillum brasilense Rhizobium Sinorhizobium meliloti
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00219193_v172_n5_p2563_Altabe
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00219193_v172_n5_p2563_Altabe
record_format	dspace
spelling	todo:paper_00219193_v172_n5_p2563_Altabe2023-10-03T14:22:46Z Expression of the Agrobacterium tumefaciens chvB virulence region in Azospirillum spp. Altabe, S. Inon de Iannino, N. De Mendoza, D. Ugalde, R.A. restriction endonuclease article Azospirillum gene expression genetic engineering membrane nonhuman priority journal Rhizobium radiobacter sequence homology virulence Agrobacterium tumefaciens Azospirillum Azospirillum brasilense Rhizobium Sinorhizobium meliloti Inner membranes of Azospirillum brasilense incubated with UDP-glucose were unable to synthesize β-(1-2) glucan and lacked the 235-kilodalton intermediate protein known to be involved in the synthesis of β-(1-2) glucan in Agrobacterium tumefaciens and Rhizobium meliloti. Inner membranes of A. brasilense strains carrying a cosmid containing the chromosomal virulence genes chvA and chvB of Agrobacterium tumefaciens formed β-(1-2) glucan in vitro and synthesized the 235-kilodalton intermediate protein. No DNA homology to the chvB region was found in different wild-type strains of A. brasilense, but the introduction of a cosmid containing the Agrobacterium tumefaciens chvA and chvB regions yielded strains in which DNA hybridization with the chvB region was detected, provided that the strains were grown under an antibiotic selective pressure. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00219193_v172_n5_p2563_Altabe
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	restriction endonuclease article Azospirillum gene expression genetic engineering membrane nonhuman priority journal Rhizobium radiobacter sequence homology virulence Agrobacterium tumefaciens Azospirillum Azospirillum brasilense Rhizobium Sinorhizobium meliloti
spellingShingle	restriction endonuclease article Azospirillum gene expression genetic engineering membrane nonhuman priority journal Rhizobium radiobacter sequence homology virulence Agrobacterium tumefaciens Azospirillum Azospirillum brasilense Rhizobium Sinorhizobium meliloti Altabe, S. Inon de Iannino, N. De Mendoza, D. Ugalde, R.A. Expression of the Agrobacterium tumefaciens chvB virulence region in Azospirillum spp.
topic_facet	restriction endonuclease article Azospirillum gene expression genetic engineering membrane nonhuman priority journal Rhizobium radiobacter sequence homology virulence Agrobacterium tumefaciens Azospirillum Azospirillum brasilense Rhizobium Sinorhizobium meliloti
description	Inner membranes of Azospirillum brasilense incubated with UDP-glucose were unable to synthesize β-(1-2) glucan and lacked the 235-kilodalton intermediate protein known to be involved in the synthesis of β-(1-2) glucan in Agrobacterium tumefaciens and Rhizobium meliloti. Inner membranes of A. brasilense strains carrying a cosmid containing the chromosomal virulence genes chvA and chvB of Agrobacterium tumefaciens formed β-(1-2) glucan in vitro and synthesized the 235-kilodalton intermediate protein. No DNA homology to the chvB region was found in different wild-type strains of A. brasilense, but the introduction of a cosmid containing the Agrobacterium tumefaciens chvA and chvB regions yielded strains in which DNA hybridization with the chvB region was detected, provided that the strains were grown under an antibiotic selective pressure.
format	JOUR
author	Altabe, S. Inon de Iannino, N. De Mendoza, D. Ugalde, R.A.
author_facet	Altabe, S. Inon de Iannino, N. De Mendoza, D. Ugalde, R.A.
author_sort	Altabe, S.
title	Expression of the Agrobacterium tumefaciens chvB virulence region in Azospirillum spp.
title_short	Expression of the Agrobacterium tumefaciens chvB virulence region in Azospirillum spp.
title_full	Expression of the Agrobacterium tumefaciens chvB virulence region in Azospirillum spp.
title_fullStr	Expression of the Agrobacterium tumefaciens chvB virulence region in Azospirillum spp.
title_full_unstemmed	Expression of the Agrobacterium tumefaciens chvB virulence region in Azospirillum spp.
title_sort	expression of the agrobacterium tumefaciens chvb virulence region in azospirillum spp.
url	http://hdl.handle.net/20.500.12110/paper_00219193_v172_n5_p2563_Altabe
work_keys_str_mv	AT altabes expressionoftheagrobacteriumtumefacienschvbvirulenceregioninazospirillumspp AT inondeianninon expressionoftheagrobacteriumtumefacienschvbvirulenceregioninazospirillumspp AT demendozad expressionoftheagrobacteriumtumefacienschvbvirulenceregioninazospirillumspp AT ugaldera expressionoftheagrobacteriumtumefacienschvbvirulenceregioninazospirillumspp
_version_	1807317185445494784

Expression of the Agrobacterium tumefaciens chvB virulence region in Azospirillum spp.

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