A putative functional vomeronasal system in anuran tadpoles
We investigated the occurrence and anatomy of the vomeronasal system (VNS) in tadpoles of 13 different anuran species. All of the species possessed a morphologically fully developed VNS with a highly conserved anatomical organisation. We found that a bean-shaped vomeronasal organ (VNO) developed ear...
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todo:paper_00218782_v221_n4_p364_Jungblut2023-10-03T14:21:44Z A putative functional vomeronasal system in anuran tadpoles Jungblut, L.D. Pozzi, A.G. Paz, D.A. Accessory olfactory system Chemical sensing G protein Pheromones Vomeronasal organ guanine nucleotide binding protein alpha subunit lectin synaptophysin animal tissue Anura article brain region embryo embryo development fetus histology larval development nonhuman olfactory bulb priority journal sensory nerve cell tadpole telencephalon vomeronasal organ Animals Anura Immunohistochemistry Larva Olfactory Bulb Species Specificity Vomeronasal Organ Tetrapoda Vertebrata We investigated the occurrence and anatomy of the vomeronasal system (VNS) in tadpoles of 13 different anuran species. All of the species possessed a morphologically fully developed VNS with a highly conserved anatomical organisation. We found that a bean-shaped vomeronasal organ (VNO) developed early in the tadpoles, during the final embryonic stages, and was located in the anteromedial nasal region. Histology revealed the presence of bipolar chemosensory neurones in the VNO that were immunoreactive for the Gαo protein. Tract-tracing experiments demonstrated that chemosensory neurones from the VNO reach specific areas in the brain, where a discernible accessory olfactory bulb (AOB) could be observed. The AOB was located in the ventrolateral side of the anterior telencephalon, somewhat caudal to the main olfactory bulb. Synaptophysin-like immunodetection revealed that synaptic contacts between VNO and AOB are established during early larval stages. Moreover, using lectin staining, we identified glomerular structures in the AOB in most of the species that we examined. According to our findings, a significant maturation in the VNS is achieved in anuran larvae. Recent published evidence strongly suggests that the VNS appeared early in vertebrate evolution and was already present in the aquatic last common ancestor of lungfish and tetrapods. In this context, tadpoles may be a good model in which to investigate the anatomical, biochemical and functional aspects of the VNS in an aquatic environment. © 2012 Anatomical Society. Fil:Jungblut, L.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pozzi, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Paz, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00218782_v221_n4_p364_Jungblut |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Accessory olfactory system Chemical sensing G protein Pheromones Vomeronasal organ guanine nucleotide binding protein alpha subunit lectin synaptophysin animal tissue Anura article brain region embryo embryo development fetus histology larval development nonhuman olfactory bulb priority journal sensory nerve cell tadpole telencephalon vomeronasal organ Animals Anura Immunohistochemistry Larva Olfactory Bulb Species Specificity Vomeronasal Organ Tetrapoda Vertebrata |
| spellingShingle |
Accessory olfactory system Chemical sensing G protein Pheromones Vomeronasal organ guanine nucleotide binding protein alpha subunit lectin synaptophysin animal tissue Anura article brain region embryo embryo development fetus histology larval development nonhuman olfactory bulb priority journal sensory nerve cell tadpole telencephalon vomeronasal organ Animals Anura Immunohistochemistry Larva Olfactory Bulb Species Specificity Vomeronasal Organ Tetrapoda Vertebrata Jungblut, L.D. Pozzi, A.G. Paz, D.A. A putative functional vomeronasal system in anuran tadpoles |
| topic_facet |
Accessory olfactory system Chemical sensing G protein Pheromones Vomeronasal organ guanine nucleotide binding protein alpha subunit lectin synaptophysin animal tissue Anura article brain region embryo embryo development fetus histology larval development nonhuman olfactory bulb priority journal sensory nerve cell tadpole telencephalon vomeronasal organ Animals Anura Immunohistochemistry Larva Olfactory Bulb Species Specificity Vomeronasal Organ Tetrapoda Vertebrata |
| description |
We investigated the occurrence and anatomy of the vomeronasal system (VNS) in tadpoles of 13 different anuran species. All of the species possessed a morphologically fully developed VNS with a highly conserved anatomical organisation. We found that a bean-shaped vomeronasal organ (VNO) developed early in the tadpoles, during the final embryonic stages, and was located in the anteromedial nasal region. Histology revealed the presence of bipolar chemosensory neurones in the VNO that were immunoreactive for the Gαo protein. Tract-tracing experiments demonstrated that chemosensory neurones from the VNO reach specific areas in the brain, where a discernible accessory olfactory bulb (AOB) could be observed. The AOB was located in the ventrolateral side of the anterior telencephalon, somewhat caudal to the main olfactory bulb. Synaptophysin-like immunodetection revealed that synaptic contacts between VNO and AOB are established during early larval stages. Moreover, using lectin staining, we identified glomerular structures in the AOB in most of the species that we examined. According to our findings, a significant maturation in the VNS is achieved in anuran larvae. Recent published evidence strongly suggests that the VNS appeared early in vertebrate evolution and was already present in the aquatic last common ancestor of lungfish and tetrapods. In this context, tadpoles may be a good model in which to investigate the anatomical, biochemical and functional aspects of the VNS in an aquatic environment. © 2012 Anatomical Society. |
| format |
JOUR |
| author |
Jungblut, L.D. Pozzi, A.G. Paz, D.A. |
| author_facet |
Jungblut, L.D. Pozzi, A.G. Paz, D.A. |
| author_sort |
Jungblut, L.D. |
| title |
A putative functional vomeronasal system in anuran tadpoles |
| title_short |
A putative functional vomeronasal system in anuran tadpoles |
| title_full |
A putative functional vomeronasal system in anuran tadpoles |
| title_fullStr |
A putative functional vomeronasal system in anuran tadpoles |
| title_full_unstemmed |
A putative functional vomeronasal system in anuran tadpoles |
| title_sort |
putative functional vomeronasal system in anuran tadpoles |
| url |
http://hdl.handle.net/20.500.12110/paper_00218782_v221_n4_p364_Jungblut |
| work_keys_str_mv |
AT jungblutld aputativefunctionalvomeronasalsysteminanurantadpoles AT pozziag aputativefunctionalvomeronasalsysteminanurantadpoles AT pazda aputativefunctionalvomeronasalsysteminanurantadpoles AT jungblutld putativefunctionalvomeronasalsysteminanurantadpoles AT pozziag putativefunctionalvomeronasalsysteminanurantadpoles AT pazda putativefunctionalvomeronasalsysteminanurantadpoles |
| _version_ |
1782024483696017408 |