The morphometric changes in the gills of the estuarine crab Chasmagnathus granulatus under hyper- and hyporegulation conditions are not caused by proliferation of specialised cells
Chasmagnathus granulatus is a hyper-hyporegulating crab that inhabits changing habitats of salinity in Brazil, Uruguay and Argentina. Since the gills are the main sites for active ion transport in crabs, the adaptive changes in the gill epithelium occurring under different conditions of salinity wer...
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todo:paper_00218782_v197_n2_p239_Genovese2023-10-03T14:21:43Z The morphometric changes in the gills of the estuarine crab Chasmagnathus granulatus under hyper- and hyporegulation conditions are not caused by proliferation of specialised cells Genovese, G. Luquet, C.M. Paz, D.A. Rosa, G.A. Pellerano, G.N. Crustacea Ion-regulation Salinity ion sea water silver nitrate acclimatization animal cell animal tissue article cell labeling cell proliferation cell size controlled study crab epithelium gill immunohistochemistry ion transport morphology morphometrics nonhuman priority journal respiratory function salinity technique thickness Adaptation, Physiological Animals Brachyura Cell Division Coloring Agents Epithelial Cells Fresh Water Gills Ion Transport Liver Pancreas Sodium Chloride Animalia Chasmagnathus granulata Crustacea Decapoda (Crustacea) Chasmagnathus granulatus is a hyper-hyporegulating crab that inhabits changing habitats of salinity in Brazil, Uruguay and Argentina. Since the gills are the main sites for active ion transport in crabs, the adaptive changes in the gill epithelium occurring under different conditions of salinity were studied by means of morphological and morphometric analysis, and immunohistochemical identification of cell proliferation (BrdU technique). In anterior (1-3) gills the epithelium thickness from crabs acclimatised to 12, 34 and 44 g/l ranged from 1.27 to 2.46 μm, with no significant change during acclimatisation, thus denoting a respiratory function. Medial (4-5) gill epithelium was slightly thicker in extreme salinities, but these differences were not statistically significant. In contrast, epithelial thickness of the posterior (6-8) gills increased significantly up to 8.10 μm (dorsal zone of gill 8) both in hyper- and hyposaline media compared with seawater. The dark areas measured in gill 8 treated with AgNO3 revealed putative ion transporting tissue, especially at 12 and 44 g/l, corresponding to the zones of higher epithelial thickness. Hence these areas seem to participate both in hyper- and hyporegulation. Proliferating cells labelled with BrdU almost never occurred in the gills/salinity combinations studied during the initial 48 h of transfer from seawater to hyperconcentrated or diluted media, thus suggesting an increase in cell size rather than cell proliferation. Fil:Genovese, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Luquet, C.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Paz, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pellerano, G.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00218782_v197_n2_p239_Genovese |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Crustacea Ion-regulation Salinity ion sea water silver nitrate acclimatization animal cell animal tissue article cell labeling cell proliferation cell size controlled study crab epithelium gill immunohistochemistry ion transport morphology morphometrics nonhuman priority journal respiratory function salinity technique thickness Adaptation, Physiological Animals Brachyura Cell Division Coloring Agents Epithelial Cells Fresh Water Gills Ion Transport Liver Pancreas Sodium Chloride Animalia Chasmagnathus granulata Crustacea Decapoda (Crustacea) |
| spellingShingle |
Crustacea Ion-regulation Salinity ion sea water silver nitrate acclimatization animal cell animal tissue article cell labeling cell proliferation cell size controlled study crab epithelium gill immunohistochemistry ion transport morphology morphometrics nonhuman priority journal respiratory function salinity technique thickness Adaptation, Physiological Animals Brachyura Cell Division Coloring Agents Epithelial Cells Fresh Water Gills Ion Transport Liver Pancreas Sodium Chloride Animalia Chasmagnathus granulata Crustacea Decapoda (Crustacea) Genovese, G. Luquet, C.M. Paz, D.A. Rosa, G.A. Pellerano, G.N. The morphometric changes in the gills of the estuarine crab Chasmagnathus granulatus under hyper- and hyporegulation conditions are not caused by proliferation of specialised cells |
| topic_facet |
Crustacea Ion-regulation Salinity ion sea water silver nitrate acclimatization animal cell animal tissue article cell labeling cell proliferation cell size controlled study crab epithelium gill immunohistochemistry ion transport morphology morphometrics nonhuman priority journal respiratory function salinity technique thickness Adaptation, Physiological Animals Brachyura Cell Division Coloring Agents Epithelial Cells Fresh Water Gills Ion Transport Liver Pancreas Sodium Chloride Animalia Chasmagnathus granulata Crustacea Decapoda (Crustacea) |
| description |
Chasmagnathus granulatus is a hyper-hyporegulating crab that inhabits changing habitats of salinity in Brazil, Uruguay and Argentina. Since the gills are the main sites for active ion transport in crabs, the adaptive changes in the gill epithelium occurring under different conditions of salinity were studied by means of morphological and morphometric analysis, and immunohistochemical identification of cell proliferation (BrdU technique). In anterior (1-3) gills the epithelium thickness from crabs acclimatised to 12, 34 and 44 g/l ranged from 1.27 to 2.46 μm, with no significant change during acclimatisation, thus denoting a respiratory function. Medial (4-5) gill epithelium was slightly thicker in extreme salinities, but these differences were not statistically significant. In contrast, epithelial thickness of the posterior (6-8) gills increased significantly up to 8.10 μm (dorsal zone of gill 8) both in hyper- and hyposaline media compared with seawater. The dark areas measured in gill 8 treated with AgNO3 revealed putative ion transporting tissue, especially at 12 and 44 g/l, corresponding to the zones of higher epithelial thickness. Hence these areas seem to participate both in hyper- and hyporegulation. Proliferating cells labelled with BrdU almost never occurred in the gills/salinity combinations studied during the initial 48 h of transfer from seawater to hyperconcentrated or diluted media, thus suggesting an increase in cell size rather than cell proliferation. |
| format |
JOUR |
| author |
Genovese, G. Luquet, C.M. Paz, D.A. Rosa, G.A. Pellerano, G.N. |
| author_facet |
Genovese, G. Luquet, C.M. Paz, D.A. Rosa, G.A. Pellerano, G.N. |
| author_sort |
Genovese, G. |
| title |
The morphometric changes in the gills of the estuarine crab Chasmagnathus granulatus under hyper- and hyporegulation conditions are not caused by proliferation of specialised cells |
| title_short |
The morphometric changes in the gills of the estuarine crab Chasmagnathus granulatus under hyper- and hyporegulation conditions are not caused by proliferation of specialised cells |
| title_full |
The morphometric changes in the gills of the estuarine crab Chasmagnathus granulatus under hyper- and hyporegulation conditions are not caused by proliferation of specialised cells |
| title_fullStr |
The morphometric changes in the gills of the estuarine crab Chasmagnathus granulatus under hyper- and hyporegulation conditions are not caused by proliferation of specialised cells |
| title_full_unstemmed |
The morphometric changes in the gills of the estuarine crab Chasmagnathus granulatus under hyper- and hyporegulation conditions are not caused by proliferation of specialised cells |
| title_sort |
morphometric changes in the gills of the estuarine crab chasmagnathus granulatus under hyper- and hyporegulation conditions are not caused by proliferation of specialised cells |
| url |
http://hdl.handle.net/20.500.12110/paper_00218782_v197_n2_p239_Genovese |
| work_keys_str_mv |
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