Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons

There is limited information about the involvement of lipids and esterified fatty acids in signaling pathways during plant development. The purpose of this study was to evaluate the lipid composition and molecular species of potato (Solanum tuberosum L., cv. Spunta) stolons and to identify phosphory...

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Autores principales:	Cenzano, A.M., Cantoro, R., Hernández-Sotomayor, S.M.T., Abdala, G.I., Racagni, G.E.
Formato:	JOUR
Lenguaje:	English
Materias:	electrospray ionization tandem mass spectrometry galactolipid kinase phospholipid potato Solanum tuberosum Electrospray ionization tandem mass spectrometry Biochemistry Enzymes Fatty acids Glycerol Ionization Mass spectrometry Metabolism Phospholipids Phosphorylation Electrospray ionization phosphotransferase vegetable protein article chemistry electrospray mass spectrometry enzymology evaluation growth, development and aging lipid metabolism metabolism methodology phosphorylation plant tuber Lipid Metabolism Phosphotransferases Plant Proteins Plant Tubers Spectrometry, Mass, Electrospray Ionization
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00218561_v60_n1_p418_Cenzano
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00218561_v60_n1_p418_Cenzano
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spelling	todo:paper_00218561_v60_n1_p418_Cenzano2023-10-03T14:21:11Z Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons Cenzano, A.M. Cantoro, R. Hernández-Sotomayor, S.M.T. Abdala, G.I. Racagni, G.E. electrospray ionization tandem mass spectrometry galactolipid kinase phospholipid potato Solanum tuberosum Electrospray ionization tandem mass spectrometry galactolipid kinase potato Solanum tuberosum Biochemistry Enzymes Fatty acids Glycerol Ionization Mass spectrometry Metabolism Phospholipids Phosphorylation Electrospray ionization phosphotransferase vegetable protein article chemistry electrospray mass spectrometry enzymology evaluation growth, development and aging lipid metabolism metabolism methodology phosphorylation plant tuber potato Lipid Metabolism Phosphorylation Phosphotransferases Plant Proteins Plant Tubers Solanum tuberosum Spectrometry, Mass, Electrospray Ionization Solanum tuberosum There is limited information about the involvement of lipids and esterified fatty acids in signaling pathways during plant development. The purpose of this study was to evaluate the lipid composition and molecular species of potato (Solanum tuberosum L., cv. Spunta) stolons and to identify phosphorylated lipids in the first two developmental stages of tuber formation. Lipid profiling was determined using ESI-MS/MS, a useful method for the determination of the biosynthesis and catabolism of lipids based on their fatty acid composition. The most prevalent compound identified in this study was phosphatidic acid (PA); digalactosyldiacylglycerol (DGDG) was the second most abundant compound. A 34:2 species was identified in PA, phosphatidylcholine (PC), phosphatidylinositol (PI), and phosphatidylethanolamine (PE). The identification of lipid phosphorylation by kinases was revealed by the presence of the phosphorylated lipids. PA was metabolized to diacylglycerol pyrophosphate (DGPP) by phosphatidic acid kinase (PAK). This work establishes a correlation between lipid fatty acid composition and lipid metabolism enzymes at the beginning of tuber formation and is the first report of PAK activity in the early events of potato tuber formation. © 2011 American Chemical Society. JOUR English info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00218561_v60_n1_p418_Cenzano
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	English
orig_language_str_mv	English
topic	electrospray ionization tandem mass spectrometry galactolipid kinase phospholipid potato Solanum tuberosum Electrospray ionization tandem mass spectrometry galactolipid kinase potato Solanum tuberosum Biochemistry Enzymes Fatty acids Glycerol Ionization Mass spectrometry Metabolism Phospholipids Phosphorylation Electrospray ionization phosphotransferase vegetable protein article chemistry electrospray mass spectrometry enzymology evaluation growth, development and aging lipid metabolism metabolism methodology phosphorylation plant tuber potato Lipid Metabolism Phosphorylation Phosphotransferases Plant Proteins Plant Tubers Solanum tuberosum Spectrometry, Mass, Electrospray Ionization Solanum tuberosum
spellingShingle	electrospray ionization tandem mass spectrometry galactolipid kinase phospholipid potato Solanum tuberosum Electrospray ionization tandem mass spectrometry galactolipid kinase potato Solanum tuberosum Biochemistry Enzymes Fatty acids Glycerol Ionization Mass spectrometry Metabolism Phospholipids Phosphorylation Electrospray ionization phosphotransferase vegetable protein article chemistry electrospray mass spectrometry enzymology evaluation growth, development and aging lipid metabolism metabolism methodology phosphorylation plant tuber potato Lipid Metabolism Phosphorylation Phosphotransferases Plant Proteins Plant Tubers Solanum tuberosum Spectrometry, Mass, Electrospray Ionization Solanum tuberosum Cenzano, A.M. Cantoro, R. Hernández-Sotomayor, S.M.T. Abdala, G.I. Racagni, G.E. Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons
topic_facet	electrospray ionization tandem mass spectrometry galactolipid kinase phospholipid potato Solanum tuberosum Electrospray ionization tandem mass spectrometry galactolipid kinase potato Solanum tuberosum Biochemistry Enzymes Fatty acids Glycerol Ionization Mass spectrometry Metabolism Phospholipids Phosphorylation Electrospray ionization phosphotransferase vegetable protein article chemistry electrospray mass spectrometry enzymology evaluation growth, development and aging lipid metabolism metabolism methodology phosphorylation plant tuber potato Lipid Metabolism Phosphorylation Phosphotransferases Plant Proteins Plant Tubers Solanum tuberosum Spectrometry, Mass, Electrospray Ionization Solanum tuberosum
description	There is limited information about the involvement of lipids and esterified fatty acids in signaling pathways during plant development. The purpose of this study was to evaluate the lipid composition and molecular species of potato (Solanum tuberosum L., cv. Spunta) stolons and to identify phosphorylated lipids in the first two developmental stages of tuber formation. Lipid profiling was determined using ESI-MS/MS, a useful method for the determination of the biosynthesis and catabolism of lipids based on their fatty acid composition. The most prevalent compound identified in this study was phosphatidic acid (PA); digalactosyldiacylglycerol (DGDG) was the second most abundant compound. A 34:2 species was identified in PA, phosphatidylcholine (PC), phosphatidylinositol (PI), and phosphatidylethanolamine (PE). The identification of lipid phosphorylation by kinases was revealed by the presence of the phosphorylated lipids. PA was metabolized to diacylglycerol pyrophosphate (DGPP) by phosphatidic acid kinase (PAK). This work establishes a correlation between lipid fatty acid composition and lipid metabolism enzymes at the beginning of tuber formation and is the first report of PAK activity in the early events of potato tuber formation. © 2011 American Chemical Society.
format	JOUR
author	Cenzano, A.M. Cantoro, R. Hernández-Sotomayor, S.M.T. Abdala, G.I. Racagni, G.E.
author_facet	Cenzano, A.M. Cantoro, R. Hernández-Sotomayor, S.M.T. Abdala, G.I. Racagni, G.E.
author_sort	Cenzano, A.M.
title	Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons
title_short	Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons
title_full	Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons
title_fullStr	Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons
title_full_unstemmed	Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons
title_sort	lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons
url	http://hdl.handle.net/20.500.12110/paper_00218561_v60_n1_p418_Cenzano
work_keys_str_mv	AT cenzanoam lipidprofilingbyelectrosprayionizationtandemmassspectrometryandtheidentificationoflipidphosphorylationbykinasesinpotatostolons AT cantoror lipidprofilingbyelectrosprayionizationtandemmassspectrometryandtheidentificationoflipidphosphorylationbykinasesinpotatostolons AT hernandezsotomayorsmt lipidprofilingbyelectrosprayionizationtandemmassspectrometryandtheidentificationoflipidphosphorylationbykinasesinpotatostolons AT abdalagi lipidprofilingbyelectrosprayionizationtandemmassspectrometryandtheidentificationoflipidphosphorylationbykinasesinpotatostolons AT racagnige lipidprofilingbyelectrosprayionizationtandemmassspectrometryandtheidentificationoflipidphosphorylationbykinasesinpotatostolons
_version_	1807314379265277952

Lipid profiling by electrospray ionization tandem mass spectrometry and the identification of lipid phosphorylation by kinases in potato stolons

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