Trypanosoma cruzi I genotypes in different geographical regions and transmission cycles based on a microsatellite motif of the intergenic spacer of spliced-leader genes
The intergenic region of spliced-leader (SL-IR) genes from 105 Trypanosoma cruzi I (Tc I) infected biological samples, culture isolates and stocks from 11 endemic countries, from Argentina to the USA were characterised, allowing identification of 76 genotypes with 54 polymorphic sites from 123 align...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00207519_v40_n14_p1599_Cura |
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todo:paper_00207519_v40_n14_p1599_Cura |
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dspace |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chagas disease Discrete typing unit Genotype PCR Spliced-leader intergenic region Trypanosoma cruzi I spacer DNA Chagas disease detection method disease transmission feces genotype geographical region polymerase chain reaction Argentina article Bolivia Brazil Central America Chagas disease Chile Colombia controlled study DNA polymorphism DNA splicing endemic disease feces analysis gene identification gene sequence genotype geographic distribution haplotype human life cycle microsatellite marker nonhuman North America nucleotide sequence opossum Paraguay parasite transmission parasite vector polymerase chain reaction protein motif sequence alignment sequence analysis South America Triatoma infestans Trypanosoma cruzi United States Animals Base Sequence Chagas Disease Disease Reservoirs DNA, Intergenic DNA, Protozoan Genotype Geography Humans Insect Vectors Microsatellite Repeats Molecular Sequence Data Phylogeny RNA, Spliced Leader Sequence Alignment Triatominae Trypanosoma cruzi Argentina Chile French Guiana Panama [Central America] United States Venezuela Didelphis virginiana Mepraia Mepraia spinolai Rhodnius nasutus Rhodnius neglectus Triatoma gerstaeckeri Triatoma infestans Trypanosoma cruzi |
| spellingShingle |
Chagas disease Discrete typing unit Genotype PCR Spliced-leader intergenic region Trypanosoma cruzi I spacer DNA Chagas disease detection method disease transmission feces genotype geographical region polymerase chain reaction Argentina article Bolivia Brazil Central America Chagas disease Chile Colombia controlled study DNA polymorphism DNA splicing endemic disease feces analysis gene identification gene sequence genotype geographic distribution haplotype human life cycle microsatellite marker nonhuman North America nucleotide sequence opossum Paraguay parasite transmission parasite vector polymerase chain reaction protein motif sequence alignment sequence analysis South America Triatoma infestans Trypanosoma cruzi United States Animals Base Sequence Chagas Disease Disease Reservoirs DNA, Intergenic DNA, Protozoan Genotype Geography Humans Insect Vectors Microsatellite Repeats Molecular Sequence Data Phylogeny RNA, Spliced Leader Sequence Alignment Triatominae Trypanosoma cruzi Argentina Chile French Guiana Panama [Central America] United States Venezuela Didelphis virginiana Mepraia Mepraia spinolai Rhodnius nasutus Rhodnius neglectus Triatoma gerstaeckeri Triatoma infestans Trypanosoma cruzi Cura, C.I. Mejía-Jaramillo, A.M. Duffy, T. Burgos, J.M. Rodriguero, M. Cardinal, M.V. Kjos, S. Gurgel-Gonçalves, R. Blanchet, D. De Pablos, L.M. Tomasini, N. da Silva, A. Russomando, G. Cuba, C.A.C. Aznar, C. Abate, T. Levin, M.J. Osuna, A. Gürtler, R.E. Diosque, P. Solari, A. Triana-Chávez, O. Schijman, A.G. Trypanosoma cruzi I genotypes in different geographical regions and transmission cycles based on a microsatellite motif of the intergenic spacer of spliced-leader genes |
| topic_facet |
Chagas disease Discrete typing unit Genotype PCR Spliced-leader intergenic region Trypanosoma cruzi I spacer DNA Chagas disease detection method disease transmission feces genotype geographical region polymerase chain reaction Argentina article Bolivia Brazil Central America Chagas disease Chile Colombia controlled study DNA polymorphism DNA splicing endemic disease feces analysis gene identification gene sequence genotype geographic distribution haplotype human life cycle microsatellite marker nonhuman North America nucleotide sequence opossum Paraguay parasite transmission parasite vector polymerase chain reaction protein motif sequence alignment sequence analysis South America Triatoma infestans Trypanosoma cruzi United States Animals Base Sequence Chagas Disease Disease Reservoirs DNA, Intergenic DNA, Protozoan Genotype Geography Humans Insect Vectors Microsatellite Repeats Molecular Sequence Data Phylogeny RNA, Spliced Leader Sequence Alignment Triatominae Trypanosoma cruzi Argentina Chile French Guiana Panama [Central America] United States Venezuela Didelphis virginiana Mepraia Mepraia spinolai Rhodnius nasutus Rhodnius neglectus Triatoma gerstaeckeri Triatoma infestans Trypanosoma cruzi |
| description |
The intergenic region of spliced-leader (SL-IR) genes from 105 Trypanosoma cruzi I (Tc I) infected biological samples, culture isolates and stocks from 11 endemic countries, from Argentina to the USA were characterised, allowing identification of 76 genotypes with 54 polymorphic sites from 123 aligned sequences. On the basis of the microsatellite motif proposed by Herrera et al. (2007) to define four haplotypes in Colombia, we could classify these genotypes into four distinct Tc I SL-IR groups, three corresponding to the former haplotypes Ia (11 genotypes), Ib (11 genotypes) and Id (35 genotypes); and one novel group, Ie (19 genotypes). Genotypes harbouring the Tc Ic motif were not detected in our study. Tc Ia was associated with domestic cycles in southern and northern South America and sylvatic cycles in Central and North America. Tc Ib was found in all transmission cycles from Colombia. Tc Id was identified in all transmission cycles from Argentina and Colombia, including Chagas cardiomyopathy patients, sylvatic Brazilian samples and human cases from French Guiana, Panama and Venezuela. Tc Ie gathered five samples from domestic Triatoma infestans from northern Argentina, nine samples from wild Mepraia spinolai and Mepraia gajardoi and two chagasic patients from Chile and one from a Bolivian patient with chagasic reactivation. Mixed infections by Tc Ia. +. Tc Id, Tc Ia. +. Tc Ie and Tc Id. +. Tc Ie were detected in vector faeces and isolates from human and vector samples. In addition, Tc Ia and Tc Id were identified in different tissues from a heart transplanted Chagas cardiomyopathy patient with reactivation, denoting histotropism. Trypanosoma cruzi I SL-IR genotypes from parasites infecting Triatoma gerstaeckeri and Didelphis virginiana from USA, T. infestans from Paraguay, Rhodnius nasutus and Rhodnius neglectus from Brazil and M. spinolai and M. gajardoi from Chile are to our knowledge described for the first time. © Australian Society for Parasitology Inc. |
| format |
JOUR |
| author |
Cura, C.I. Mejía-Jaramillo, A.M. Duffy, T. Burgos, J.M. Rodriguero, M. Cardinal, M.V. Kjos, S. Gurgel-Gonçalves, R. Blanchet, D. De Pablos, L.M. Tomasini, N. da Silva, A. Russomando, G. Cuba, C.A.C. Aznar, C. Abate, T. Levin, M.J. Osuna, A. Gürtler, R.E. Diosque, P. Solari, A. Triana-Chávez, O. Schijman, A.G. |
| author_facet |
Cura, C.I. Mejía-Jaramillo, A.M. Duffy, T. Burgos, J.M. Rodriguero, M. Cardinal, M.V. Kjos, S. Gurgel-Gonçalves, R. Blanchet, D. De Pablos, L.M. Tomasini, N. da Silva, A. Russomando, G. Cuba, C.A.C. Aznar, C. Abate, T. Levin, M.J. Osuna, A. Gürtler, R.E. Diosque, P. Solari, A. Triana-Chávez, O. Schijman, A.G. |
| author_sort |
Cura, C.I. |
| title |
Trypanosoma cruzi I genotypes in different geographical regions and transmission cycles based on a microsatellite motif of the intergenic spacer of spliced-leader genes |
| title_short |
Trypanosoma cruzi I genotypes in different geographical regions and transmission cycles based on a microsatellite motif of the intergenic spacer of spliced-leader genes |
| title_full |
Trypanosoma cruzi I genotypes in different geographical regions and transmission cycles based on a microsatellite motif of the intergenic spacer of spliced-leader genes |
| title_fullStr |
Trypanosoma cruzi I genotypes in different geographical regions and transmission cycles based on a microsatellite motif of the intergenic spacer of spliced-leader genes |
| title_full_unstemmed |
Trypanosoma cruzi I genotypes in different geographical regions and transmission cycles based on a microsatellite motif of the intergenic spacer of spliced-leader genes |
| title_sort |
trypanosoma cruzi i genotypes in different geographical regions and transmission cycles based on a microsatellite motif of the intergenic spacer of spliced-leader genes |
| url |
http://hdl.handle.net/20.500.12110/paper_00207519_v40_n14_p1599_Cura |
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1782026783056461824 |
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todo:paper_00207519_v40_n14_p1599_Cura2023-10-03T14:18:35Z Trypanosoma cruzi I genotypes in different geographical regions and transmission cycles based on a microsatellite motif of the intergenic spacer of spliced-leader genes Cura, C.I. Mejía-Jaramillo, A.M. Duffy, T. Burgos, J.M. Rodriguero, M. Cardinal, M.V. Kjos, S. Gurgel-Gonçalves, R. Blanchet, D. De Pablos, L.M. Tomasini, N. da Silva, A. Russomando, G. Cuba, C.A.C. Aznar, C. Abate, T. Levin, M.J. Osuna, A. Gürtler, R.E. Diosque, P. Solari, A. Triana-Chávez, O. Schijman, A.G. Chagas disease Discrete typing unit Genotype PCR Spliced-leader intergenic region Trypanosoma cruzi I spacer DNA Chagas disease detection method disease transmission feces genotype geographical region polymerase chain reaction Argentina article Bolivia Brazil Central America Chagas disease Chile Colombia controlled study DNA polymorphism DNA splicing endemic disease feces analysis gene identification gene sequence genotype geographic distribution haplotype human life cycle microsatellite marker nonhuman North America nucleotide sequence opossum Paraguay parasite transmission parasite vector polymerase chain reaction protein motif sequence alignment sequence analysis South America Triatoma infestans Trypanosoma cruzi United States Animals Base Sequence Chagas Disease Disease Reservoirs DNA, Intergenic DNA, Protozoan Genotype Geography Humans Insect Vectors Microsatellite Repeats Molecular Sequence Data Phylogeny RNA, Spliced Leader Sequence Alignment Triatominae Trypanosoma cruzi Argentina Chile French Guiana Panama [Central America] United States Venezuela Didelphis virginiana Mepraia Mepraia spinolai Rhodnius nasutus Rhodnius neglectus Triatoma gerstaeckeri Triatoma infestans Trypanosoma cruzi The intergenic region of spliced-leader (SL-IR) genes from 105 Trypanosoma cruzi I (Tc I) infected biological samples, culture isolates and stocks from 11 endemic countries, from Argentina to the USA were characterised, allowing identification of 76 genotypes with 54 polymorphic sites from 123 aligned sequences. On the basis of the microsatellite motif proposed by Herrera et al. (2007) to define four haplotypes in Colombia, we could classify these genotypes into four distinct Tc I SL-IR groups, three corresponding to the former haplotypes Ia (11 genotypes), Ib (11 genotypes) and Id (35 genotypes); and one novel group, Ie (19 genotypes). Genotypes harbouring the Tc Ic motif were not detected in our study. Tc Ia was associated with domestic cycles in southern and northern South America and sylvatic cycles in Central and North America. Tc Ib was found in all transmission cycles from Colombia. Tc Id was identified in all transmission cycles from Argentina and Colombia, including Chagas cardiomyopathy patients, sylvatic Brazilian samples and human cases from French Guiana, Panama and Venezuela. Tc Ie gathered five samples from domestic Triatoma infestans from northern Argentina, nine samples from wild Mepraia spinolai and Mepraia gajardoi and two chagasic patients from Chile and one from a Bolivian patient with chagasic reactivation. Mixed infections by Tc Ia. +. Tc Id, Tc Ia. +. Tc Ie and Tc Id. +. Tc Ie were detected in vector faeces and isolates from human and vector samples. In addition, Tc Ia and Tc Id were identified in different tissues from a heart transplanted Chagas cardiomyopathy patient with reactivation, denoting histotropism. Trypanosoma cruzi I SL-IR genotypes from parasites infecting Triatoma gerstaeckeri and Didelphis virginiana from USA, T. infestans from Paraguay, Rhodnius nasutus and Rhodnius neglectus from Brazil and M. spinolai and M. gajardoi from Chile are to our knowledge described for the first time. © Australian Society for Parasitology Inc. Fil:Duffy, T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Burgos, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodriguero, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cardinal, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gürtler, R.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Schijman, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00207519_v40_n14_p1599_Cura |