Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease
Congenital transmission of Trypanosoma cruzi may occur in some or all the gestations from a T. cruzi-infected mother. Variable rates of congenital transmission have been reported in different geographical areas where different parasitic strains predominate, suggesting that parasitic genotypes might...
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todo:paper_00207519_v37_n12_p1319_Burgos2023-10-03T14:18:33Z Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease Burgos, J.M. Altcheh, J. Bisio, M. Duffy, T. Valadares, H.M.S. Seidenstein, M.E. Piccinali, R. Freitas, J.M. Levin, M.J. Macchi, L. Macedo, A.M. Freilij, H. Schijman, A.G. Microsatellite loci Phylogenetic lineage Real time PCR Transplacental transmission Trypanosoma cruzi clonality minicircle DNA allele blood Chagas disease disease transmission genetic marker genotype molecular analysis parasite transmission phylogenetics polymerase chain reaction polymorphism pregnancy protozoan adult Argentina article blood sampling Bolivia Chagas disease child congenital infection controlled study endemic disease female gene amplification gene locus genetic analysis genetic line genotype human infant major clinical study newborn parasite transmission polymerase chain reaction prevalence restriction fragment length polymorphism Trypanosoma Trypanosoma cruzi twins vertical transmission Adolescent Adult Animals Argentina Bolivia Chagas Disease Child Child, Preschool Disease Susceptibility Disease Transmission, Vertical DNA, Protozoan Female Humans Infant Infant, Newborn Male Mothers Polymerase Chain Reaction Pregnancy Pregnancy Complications, Parasitic Risk Factors Trypanosoma cruzi Argentina Bolivia South America Trypanosoma cruzi Congenital transmission of Trypanosoma cruzi may occur in some or all the gestations from a T. cruzi-infected mother. Variable rates of congenital transmission have been reported in different geographical areas where different parasitic strains predominate, suggesting that parasitic genotypes might play a role in the risk of congenital transmission. Moreover, in cases of transmission it is unknown if the whole maternal T. cruzi population or certain clones are preferentially transmitted by the transplacental route. In this study, bloodstream T. cruzi lineages were identified in blood samples from congenitally infected children, transmitting and non-transmitting mothers and unrelated Chagas disease patients, using improved PCR strategies targeted to nuclear genomic markers. T. cruzi IId was the prevalent genotype among 36/38 PCR-positive congenitally infected infants, 5/5 mothers who transmitted congenital Chagas disease, 12/13 mothers who delivered non-infected children and 28/34 unrelated Chagas disease patients, all coming from endemic localities of Argentina and Bolivia. These figures indicate no association between a particular genotype and vertical transmission. Furthermore, minicircle signatures from the maternal and infants' bloodstream trypanosomes were profiled by restriction fragment length polymorphism of the 330-bp PCR-amplified variable regions in seven cases of mothers and congenitally infected infants. Minicircle signatures were nearly identical between each mother and her infant/s and unique to each mother-infant/s case, a feature that was also observed in twin deliveries. Moreover, allelic size polymorphism analysis of microsatellite loci from populations transmitted to twins showed that all clones from the maternal polyclonal population were equally infective to both siblings. © 2007 Australian Society for Parasitology Inc. Fil:Burgos, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Duffy, T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Piccinali, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Schijman, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00207519_v37_n12_p1319_Burgos |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Microsatellite loci Phylogenetic lineage Real time PCR Transplacental transmission Trypanosoma cruzi clonality minicircle DNA allele blood Chagas disease disease transmission genetic marker genotype molecular analysis parasite transmission phylogenetics polymerase chain reaction polymorphism pregnancy protozoan adult Argentina article blood sampling Bolivia Chagas disease child congenital infection controlled study endemic disease female gene amplification gene locus genetic analysis genetic line genotype human infant major clinical study newborn parasite transmission polymerase chain reaction prevalence restriction fragment length polymorphism Trypanosoma Trypanosoma cruzi twins vertical transmission Adolescent Adult Animals Argentina Bolivia Chagas Disease Child Child, Preschool Disease Susceptibility Disease Transmission, Vertical DNA, Protozoan Female Humans Infant Infant, Newborn Male Mothers Polymerase Chain Reaction Pregnancy Pregnancy Complications, Parasitic Risk Factors Trypanosoma cruzi Argentina Bolivia South America Trypanosoma cruzi |
spellingShingle |
Microsatellite loci Phylogenetic lineage Real time PCR Transplacental transmission Trypanosoma cruzi clonality minicircle DNA allele blood Chagas disease disease transmission genetic marker genotype molecular analysis parasite transmission phylogenetics polymerase chain reaction polymorphism pregnancy protozoan adult Argentina article blood sampling Bolivia Chagas disease child congenital infection controlled study endemic disease female gene amplification gene locus genetic analysis genetic line genotype human infant major clinical study newborn parasite transmission polymerase chain reaction prevalence restriction fragment length polymorphism Trypanosoma Trypanosoma cruzi twins vertical transmission Adolescent Adult Animals Argentina Bolivia Chagas Disease Child Child, Preschool Disease Susceptibility Disease Transmission, Vertical DNA, Protozoan Female Humans Infant Infant, Newborn Male Mothers Polymerase Chain Reaction Pregnancy Pregnancy Complications, Parasitic Risk Factors Trypanosoma cruzi Argentina Bolivia South America Trypanosoma cruzi Burgos, J.M. Altcheh, J. Bisio, M. Duffy, T. Valadares, H.M.S. Seidenstein, M.E. Piccinali, R. Freitas, J.M. Levin, M.J. Macchi, L. Macedo, A.M. Freilij, H. Schijman, A.G. Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease |
topic_facet |
Microsatellite loci Phylogenetic lineage Real time PCR Transplacental transmission Trypanosoma cruzi clonality minicircle DNA allele blood Chagas disease disease transmission genetic marker genotype molecular analysis parasite transmission phylogenetics polymerase chain reaction polymorphism pregnancy protozoan adult Argentina article blood sampling Bolivia Chagas disease child congenital infection controlled study endemic disease female gene amplification gene locus genetic analysis genetic line genotype human infant major clinical study newborn parasite transmission polymerase chain reaction prevalence restriction fragment length polymorphism Trypanosoma Trypanosoma cruzi twins vertical transmission Adolescent Adult Animals Argentina Bolivia Chagas Disease Child Child, Preschool Disease Susceptibility Disease Transmission, Vertical DNA, Protozoan Female Humans Infant Infant, Newborn Male Mothers Polymerase Chain Reaction Pregnancy Pregnancy Complications, Parasitic Risk Factors Trypanosoma cruzi Argentina Bolivia South America Trypanosoma cruzi |
description |
Congenital transmission of Trypanosoma cruzi may occur in some or all the gestations from a T. cruzi-infected mother. Variable rates of congenital transmission have been reported in different geographical areas where different parasitic strains predominate, suggesting that parasitic genotypes might play a role in the risk of congenital transmission. Moreover, in cases of transmission it is unknown if the whole maternal T. cruzi population or certain clones are preferentially transmitted by the transplacental route. In this study, bloodstream T. cruzi lineages were identified in blood samples from congenitally infected children, transmitting and non-transmitting mothers and unrelated Chagas disease patients, using improved PCR strategies targeted to nuclear genomic markers. T. cruzi IId was the prevalent genotype among 36/38 PCR-positive congenitally infected infants, 5/5 mothers who transmitted congenital Chagas disease, 12/13 mothers who delivered non-infected children and 28/34 unrelated Chagas disease patients, all coming from endemic localities of Argentina and Bolivia. These figures indicate no association between a particular genotype and vertical transmission. Furthermore, minicircle signatures from the maternal and infants' bloodstream trypanosomes were profiled by restriction fragment length polymorphism of the 330-bp PCR-amplified variable regions in seven cases of mothers and congenitally infected infants. Minicircle signatures were nearly identical between each mother and her infant/s and unique to each mother-infant/s case, a feature that was also observed in twin deliveries. Moreover, allelic size polymorphism analysis of microsatellite loci from populations transmitted to twins showed that all clones from the maternal polyclonal population were equally infective to both siblings. © 2007 Australian Society for Parasitology Inc. |
format |
JOUR |
author |
Burgos, J.M. Altcheh, J. Bisio, M. Duffy, T. Valadares, H.M.S. Seidenstein, M.E. Piccinali, R. Freitas, J.M. Levin, M.J. Macchi, L. Macedo, A.M. Freilij, H. Schijman, A.G. |
author_facet |
Burgos, J.M. Altcheh, J. Bisio, M. Duffy, T. Valadares, H.M.S. Seidenstein, M.E. Piccinali, R. Freitas, J.M. Levin, M.J. Macchi, L. Macedo, A.M. Freilij, H. Schijman, A.G. |
author_sort |
Burgos, J.M. |
title |
Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease |
title_short |
Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease |
title_full |
Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease |
title_fullStr |
Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease |
title_full_unstemmed |
Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease |
title_sort |
direct molecular profiling of minicircle signatures and lineages of trypanosoma cruzi bloodstream populations causing congenital chagas disease |
url |
http://hdl.handle.net/20.500.12110/paper_00207519_v37_n12_p1319_Burgos |
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