Polypyrrole intermediates in porphyrin biosynthesis. Studies with Euglena gracilis

1. 1. Evidence for the formation of pyrrolic intermediates resulting from the action of a whole enzyme system and deaminase preparations from Euglena gracilis on porphobilinogen, under normal anaerobic conditions is reported. 2. 2. It was found that the activity of Euglena gracilis pellet was not af...

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Autores principales: Rossetti, M.V., Del C. Batlle, A.M.
Formato: JOUR
Materias:
porphyrin
euglena gracilis
in vitro study
microorganism
theoretical study
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0020711X_v8_n4_p277_Rossetti
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_0020711X_v8_n4_p277_Rossetti2023-10-03T14:18:12Z Polypyrrole intermediates in porphyrin biosynthesis. Studies with Euglena gracilis Rossetti, M.V. Del C. Batlle, A.M. porphyrin euglena gracilis in vitro study microorganism theoretical study 1. 1. Evidence for the formation of pyrrolic intermediates resulting from the action of a whole enzyme system and deaminase preparations from Euglena gracilis on porphobilinogen, under normal anaerobic conditions is reported. 2. 2. It was found that the activity of Euglena gracilis pellet was not affected by the presence of air; the yield of porphyrinogens being the same under anaerobiosis and aerobiosis. Both with pellet and deaminase preparations, two phases in porphyrinogen synthesis were observed, a slow and a rapid one, suggesting that some intermediates in the reaction were formed. 3. 3. The nature of these intermediates was investigated by incubating them with different enzyme preparations and PBG. Results obtained suggested that the polypyrroles formed from the whole system and deaminase were different. They did not behave as substrates but as intermediates m the reaction and significantly inhibited total porphyrinogen formation. It is proposed that these intermediates could be in high percentage larger polypyrrylmethanes. © 1977. Fil:Rossetti, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0020711X_v8_n4_p277_Rossetti
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic porphyrin
euglena gracilis
in vitro study
microorganism
theoretical study
spellingShingle porphyrin
euglena gracilis
in vitro study
microorganism
theoretical study
Rossetti, M.V.
Del C. Batlle, A.M.
Polypyrrole intermediates in porphyrin biosynthesis. Studies with Euglena gracilis
topic_facet porphyrin
euglena gracilis
in vitro study
microorganism
theoretical study
description 1. 1. Evidence for the formation of pyrrolic intermediates resulting from the action of a whole enzyme system and deaminase preparations from Euglena gracilis on porphobilinogen, under normal anaerobic conditions is reported. 2. 2. It was found that the activity of Euglena gracilis pellet was not affected by the presence of air; the yield of porphyrinogens being the same under anaerobiosis and aerobiosis. Both with pellet and deaminase preparations, two phases in porphyrinogen synthesis were observed, a slow and a rapid one, suggesting that some intermediates in the reaction were formed. 3. 3. The nature of these intermediates was investigated by incubating them with different enzyme preparations and PBG. Results obtained suggested that the polypyrroles formed from the whole system and deaminase were different. They did not behave as substrates but as intermediates m the reaction and significantly inhibited total porphyrinogen formation. It is proposed that these intermediates could be in high percentage larger polypyrrylmethanes. © 1977.
format JOUR
author Rossetti, M.V.
Del C. Batlle, A.M.
author_facet Rossetti, M.V.
Del C. Batlle, A.M.
author_sort Rossetti, M.V.
title Polypyrrole intermediates in porphyrin biosynthesis. Studies with Euglena gracilis
title_short Polypyrrole intermediates in porphyrin biosynthesis. Studies with Euglena gracilis
title_full Polypyrrole intermediates in porphyrin biosynthesis. Studies with Euglena gracilis
title_fullStr Polypyrrole intermediates in porphyrin biosynthesis. Studies with Euglena gracilis
title_full_unstemmed Polypyrrole intermediates in porphyrin biosynthesis. Studies with Euglena gracilis
title_sort polypyrrole intermediates in porphyrin biosynthesis. studies with euglena gracilis
url http://hdl.handle.net/20.500.12110/paper_0020711X_v8_n4_p277_Rossetti
work_keys_str_mv AT rossettimv polypyrroleintermediatesinporphyrinbiosynthesisstudieswitheuglenagracilis
AT delcbatlleam polypyrroleintermediatesinporphyrinbiosynthesisstudieswitheuglenagracilis
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